DDX11 (NM_001257144) Human Untagged Clone

SKU SC332506

DDX11 (untagged) - Homo sapiens DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (DDX11), transcript variant 4

Product Data

• Type: Human Untagged Clone
• Target Symbol: DDX11
• Synonyms: CHL1; CHLR1; KRG2; WABS
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC332506 representing NM_001257144.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; ATGGCTAATGAAACACAGAAGGTTGGTGCCATCCATTTTCCTTTTCCCTTCACACCCTATTCCATCCAG; GAAGACTTCATGGCAGAGCTGTACCGGGTTTTGGAGGCTGGCAAGATTGGGATATTTGAGAGTCCAACT; GGCACTGGGAAGTCCTTAAGTCTTATTTGTGGGGCCCTCTCTTGGCTCCGTGACTTTGAACAGAAGAAG; CGTGAAGAAGAGGCACGACTCCTTGAAACTGGAACTGGCCCCTTACATGATGAGAAAGATGAATCCCTG; TGTCTGTCTTCTTCCTGCGAAGGGGCTGCAGGCACCCCGAGGCCTGCTGGAGAACCGGCCTGGGTTACT; CAGTTTGTGCAGAAGAAAGAAGAGAGGGACCTGGTGGACCGACTAAAGGCGGAGCAGGCCAGGAGGAAG; CAGCGAGAAGAACGCCTGCAGCAGCTGCAGCACAGGGTGCAGCTCAAGTATGCAGCCAAGCGCCTGAGG; CAGGAAGAAGAAGAAAGAGAGAATCTCCTCCGCCTCAGCAGGGAGATGCTAGAGACAGGCCCGGAGGCT; GAGCGGCTGGAGCAGCTGGAGTCTGGGGAGGAGGAGCTGGTCCTCGCCGAATACGAGAGTGATGAGGAG; AAAAAGGTGGCGAGCAGAGTGGATGAGGATGAGGATGACCTGGAGGAAGAACACATAACTAAGATTTAT; TACTGTAGTCGGACACACTCCCAGCTGGCCCAGTTTGTGCATGAGGTGAAGAAGAGCCCCTTTGGCAAG; GATGTTCGGCTGGTCTCCCTTGGCTCCCGGCAGAACCTTTGTGTAAATGAAGACGTGAAAAGCCTAGGT; TCTGTGCAGCTTATCAACGACCGCTGTGTGGACATGCAGAGAAGCAGGCACGAGAAGAAGAAAGGAGCT; GAGGAGGAGAAGCCAAAGAGGAGGAGGCAGGAGAAGCAGGCAGCCTGCCCCTTCTACAACCACGAGCAG; ATGGGCCTTCTCCGGGATGAGGCCCTGGCAGAGGTGAAGGACATGGAGCAGCTGCTGGCCCTTGGGAAG; GAGGCCCGGGCCTGTCCCTATTACGGGAGCCGCCTTGCCATCCCTGCAGCCCAGCTGGTGGTGCTGCCC; TATCAGATGCTGCTGCATGCGGCCACTCGGCAGGCCGCGGGCATCCGGCTGCAGGACCAGGTGGTGATC; ATCGACGAGGCGCACAACCTGATCGACACCATCACGGGCATGCACAGCGTGGAGGTCAGCGGCTCCCAG; CTCTGCCAGGCCCATTCCCAGCTGCTGCAGTACGTGGAGCGATACGGGAAGCGTTTGAAGGCCAAGAAC; CTGATGTACCTGAAGCAGATCCTGTATTTGCTGGAGAAATTCGTGGCTGTGCTAGGGGGGAACATTAAG; CAAAATCCCAATACACAGAGTCTGTCACAGACAGGGACGGAGCTGAAGACCATCAACGACTTTCTCTTC; CAGAGCCAGATCGACAACATCAACCTGTTCAAGGTGCAGCGATACTGTGAGAAGAGCATGATCAGCAGA; AAGCTCTTTGGATTCACTGAACGGTACGGAGCAGTGTTCTCATCCCGGGAGCAGCCCAAACTGGCTGGG; TTTCAGCAATTCCTGCAGAGCCTGCAGCCCAGGACGACTGAAGCTCTTGCAGCCCCTGCAGACGAGAGT; CAGGCCAGCACCCTGCGACCAGCTTCTCCACTGATGCACATCCAAGGCTTCCTGGCAGCTCTCACTACG; GCCAACCAGGACGGCAGGGTCATCCTGAGCCGCCAAGGCAGCCTCAGTCAGAGCACCCTGAAGTTTTTG; CTCCTGAATCCAGCTGTGCACTTTGCCCAAGTGGTGAAGGAATGCCGGGCAGTGGTCATTGCGGGGGGT; ACCATGCAGCCGGTGTCTGACTTCCGGCAGCAGCTGCTGGCCTGTGCCGGGGTGGAAGCTGAGCGCGTG; GTGGAGTTTTCCTGTGGTCACGTGATCCCTCCAGACAACATCCTGCCCCTCGTCATCTGCAGCGGGATC; TCCAACCAGCCGCTGGAATTCACGTTCCAGAAAAGAGAGCTGCCTCAGATGATGGACGAGGTGGGTCGC; ATTCTCTGTAACCTGTGCGGTGTGGTTCCTGGAGGGGTGGTCTGTTTCTTCCCCTCCTACGAGTACCTG; CGCCAGGTCCATGCCCACTGGGAGAAGGGTGGCCTGCTGGGCCGTCTGGCTGCCAGGAAGAAGATATTC; CAGGAACCTAAGAGCGCACACCAGGTGGAGCAGGTGCTGCTGGCATATTCCAGGTGCATCCAGGCCTGT; GGCCAGGAGAGAGGCCAGGTGACAGGGGCCCTGCTCCTCTCTGTGGTTGGAGGAAAGATGAGTGAAGGG; ATCAACTTCTCTGACAACCTAGGCCGGTGTGTGGTGATGGTGGGCATGCCCTTCCCCAACATCAGGTCT; GCAGAGCTGCAGGAGAAGATGGCCTACTTGGATCAAACCCTCAGCCCCCGGCCAGGCACCCCCAGGGAA; GGCTCTGGTGGAGAACCTGTGCATGAAGGCCGTCAACCAGTCCATAGGCAGGGCCATCAGGCACCAGAA; GGATTTTGCCAGCGTAGTGCTCCTGGACCAGCGATATGCCCGGCCCCCTGTCCTGGCCAAGCTGCCGGC; CTGGATCCGAGCCCGTGTGGAGGTCAAAGCTACCTTTGGCCCCGCCATTGCTGCTGTGCAGAAGTTTCA; CCGGGAGAAGTCGGCCTCTTCCTGATGGGCAACCACACCACTGCCTGGCGCCGTGCCCTTCCTTTGTCC; TGCCCGCTGGAGACAGTGTTTGTCGTGGGCGTGGTCTGCGGGGATCCTGTTACAAAGGTGAAACCCAGG; AGGAGAGTGTGGAGTCCAGAGTGCTGCCAGGACCCAGGCACAGGCGTTAGCTCCCGTAGGAGAAAATGG; GGGAATCCTGAATGA;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001257144
• Insert Size: 2913 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001257144.1
• RefSeq Size: 3823 bp
• RefSeq ORF: 2913 bp
• Locus ID: 1663
• UniProt ID: Q96FC9
• Cytogenetics: 12p11.21
• Protein Families: Stem cell - Pluripotency
• MW: 108.3 kDa
• Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 3. Variants 3 and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.