MEGF10 (NM_001256545) Human Untagged Clone

SKU SC332415

MEGF10 (untagged) - Homo sapiens multiple EGF-like-domains 10 (MEGF10), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: MEGF10
• Synonyms: EMARDD; SR-F3
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC332415 representing NM_001256545.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; ATGGTTATTTCTTTGAACTCATGCCTGAGCTTTATTTGTTTATTGTTATGCCACTGGATTGGGACAGCA; TCACCTCTGAATCTTGAAGACCCTAATGTGTGTAGCCACTGGGAAAGCTACTCAGTGACTGTGCAAGAG; TCATACCCACATCCCTTTGATCAAATTTACTACACGAGCTGCACTGACATTCTAAACTGGTTTAAATGC; ACGCGGCACAGAGTCAGCTATCGGACAGCCTATCGACATGGGGAGAAGACTATGTATAGGCGCAAGTCT; CAGTGTTGTCCTGGATTTTATGAAAGCGGGGAAATGTGTGTCCCCCACTGTGCTGATAAATGTGTCCAT; GGTCGCTGTATTGCTCCAAACACCTGTCAGTGTGAGCCTGGCTGGGGAGGGACCAACTGCTCCAGTGCC; TGCGATGGTGATCACTGGGGTCCCCACTGCACCAGCCGGTGCCAGTGCAAAAATGGGGCTCTGTGCAAC; CCCATCACCGGGGCTTGCCACTGTGCTGCGGGCTTCCGGGGCTGGCGCTGCGAGGACCGCTGTGAGCAG; GGCACCTATGGTAACGACTGTCATCAGAGATGCCAGTGCCAGAATGGAGCCACCTGCGACCACGTCACG; GGGGAATGCCGCTGCCCACCAGGATACACCGGAGCCTTCTGTGAGGATCTTTGTCCTCCTGGTAAACAT; GGTCCACAGTGTGAGCAGAGATGCCCTTGTCAAAATGGAGGAGTGTGTCATCACGTCACTGGAGAATGC; TCTTGCCCTTCTGGCTGGATGGGCACAGTGTGTGGTCAGCCTTGCCCCGAGGGTCGCTTTGGAAAGAAC; TGTTCCCAAGAATGCCAGTGCCATAATGGAGGGACGTGTGATGCTGCCACAGGCCAATGTCATTGCAGT; CCAGGATACACAGGGGAACGGTGCCAGGATGAGTGTCCTGTTGGGACCTATGGCGTTCTCTGTGCTGAG; ACCTGCCAGTGTGTCAACGGAGGGAAGTGTTACCACGTGAGCGGCGCATGCCTCTGTGAAGCAGGCTTT; GCTGGCGAGCGCTGCGAAGCACGCCTGTGTCCTGAGGGGCTCTACGGCATCAAATGTGACAAACGGTGT; CCCTGCCACCTGGAAAACACTCATAGCTGTCACCCCATGTCTGGAGAGTGTGCCTGCAAGCCGGGCTGG; TCAGGACTCTACTGTAATGAGACATGTTCTCCTGGATTCTACGGGGAAGCTTGCCAGCAGATCTGCAGC; TGCCAAAATGGGGCAGACTGTGACAGTGTGACTGGAAAGTGCACCTGTGCCCCAGGATTCAAAGGAATT; GACTGCTCTACCCCATGCCCTCTGGGAACCTATGGGATAAACTGTTCCTCTCGCTGTGGCTGTAAAAAT; GATGCAGTCTGCTCTCCTGTGGACGGGTCTTGTACTTGCAAGGCAGGCTGGCACGGGGTGGACTGCTCC; ATCAGATGTCCCAGTGGCACATGGGGCTTTGGCTGTAACTTAACATGCCAGTGCCTCAACGGGGGAGCC; TGCAACACCCTGGACGGGACCTGCACGTGTGCACCTGGATGGCGCGGGGAGAAATGCGAACTTCCCTGC; CAGGATGGCACGTACGGGCTGAACTGTGCTGAGCGCTGCGACTGCAGCCACGCAGATGGCTGCCACCCT; ACCACGGGCCATTGCCGCTGCCTCCCCGGATGGTCAGGTGTCCACTGTGACAGCGTGTGTGCTGAGGGA; CGCTGGGGCCCCAACTGCTCCCTGCCCTGCTACTGTAAAAATGGGGCTTCATGCTCCCCTGATGATGGC; ATCTGCGAGTGTGCACCAGGCTTCCGAGGCACCACTTGTCAGAGGATCTGCTCCCCTGGTTTTTATGGG; CATCGCTGCAGCCAGACATGCCCACAGTGCGTTCACAGCAGCGGGCCCTGCCACCACATCACCGGCCTG; TGTGACTGCTTGCCTGGCTTCACAGGCGCCCTCTGCAATGAAGTGTGTCCCAGTGGCAGATTTGGGAAA; AACTGTGCAGGAATTTGTACCTGCACCAACAACGGAACCTGTAACCCCATTGACAGATCTTGTCAGTGT; TACCCCGGTTGGATTGGCAGTGACTGCTCTCAACCATGTCCACCTGCCCACTGGGGCCCAAACTGCATC; CACACGTGCAACTGCCATAATGGAGCTTTCTGCAGCGCCTACGATGGGGAATGTAAATGCACTCCTGGC; TGGACAGGGCTCTACTGCACTCAGAGATGTCCTCTAGGGTTTTATGGAAAAGATTGTGCACTGATATGC; CAATGTCAAAACGGAGCTGACTGCGACCACATTTCTGGGCAGTGTACTTGCCGCACTGGATTCATGGGA; CGGCACTGTGAGCAGAAGTGCCCTTCAGGAACATATGGCTATGGCTGTCGCCAGATATGTGATTGTCTG; AACAACTCCACCTGCGACCACATCACTGGGACCTGTTACTGCAGCCCCGGATGGAAGGGAGCGAGATGT; GATCAAGCTGGTGTTATCATAGTTGGAAATCTGAACAGCTTAAGCCGAACCAGTACTGCTCTCCCTGCT; GATTCCTACCAGATCGGGGCCATTGCAGGCATCATCATTCTTGTCCTAGTTGTTCTCTTCCTACTGGCA; TTGTTCATTATTTATAGACACAAGCAGAAGGGAAAGGAATCAAGCATGCCAGCAGTTACCTACACCCCT; GCTATGAGGGTCGTCAATGCAGATTATACCATTTCAGGAACCCTTCCTCACAGCAATGGTGGAAACGCT; AATAGCCACTACTTCACCAATCCCAGTTACCACACGCTCACCCAGTGTGCCACATCCCCTCACGTCAAC; AACAGGGACAGGATGACTGTCACGAAGTCAAAAAACAATCAACTGTTTGTGAATCTTAAAAATGTGAAC; CCTGGGAAGAGAGGCCCTGTGGGGGACTGCACTGGGACATTGCCGGCTGACTGGAAACATGGCGGCTAC; CTCAACGAGCTCGGTGCTTTTGGACTTGACAGAAGCTATATGGGAAAATCCTTAAAAGACCTGGGAAAG; AATTCTGAATATAATTCAAGTAACTGCTCCCTAAGCAGTTCTGAGAACCCATATGCCACTATTAAAGAC; CCACCTGTACTTATCCCGAAAAGCTCAGAGTGTGGTTATGTGGAGATGAAATCGCCGGCACGAAGAGAT; TCCCCATATGCAGAGATCAATAACTCAACTTCAGCCAACAGGAATGTCTATGAAGTTGAACCTACAGTG; AGTGTTGTCCAAGGAGTATTCAGCAATAATGGGCGTCTCTCCCAGGATCCATATGACCTCCCAAAGAAC; AGTCACATCCCTTGTCATTATGACCTGCTGCCAGTCCGAGACAGTTCATCCTCCCCTAAGCAAGAGGAC; AGTGGTGGTAGCAGCAGCAACAGCAGCAGCAGCAGTGAATGA;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001256545
• Insert Size: 3423 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001256545.1
• RefSeq Size: 7638 bp
• RefSeq ORF: 3423 bp
• Locus ID: 84466
• UniProt ID: Q96KG7
• Cytogenetics: 5q23.2
• Protein Families: Transmembrane
• MW: 122.2 kDa
• Summary: This gene encodes a member of the multiple epidermal growth factor-like domains protein family. The encoded protein plays a role in cell adhesion, motility and proliferation, and is a critical mediator of apoptotic cell phagocytosis as well as amyloid-beta peptide uptake in the brain. Expression of this gene may be associated with schizophrenia, and mutations in this gene are a cause of early-onset myopathy, areflexia, respiratory distress, and dysphagia (EMARDD) as well as congenital myopathy with minicores. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Apr 2012]; Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.