Prolactin Receptor (PRLR) (NM_001204314) Human Untagged Clone

SKU SC331558

PRLR (untagged) - Homo sapiens prolactin receptor (PRLR), transcript variant 6

Product Data

• Type: Human Untagged Clone
• Target Symbol: Prolactin Receptor
• Synonyms: HPRL; hPRLrI; MFAB; RI-PRLR
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC331558 representing NM_001204314.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; ATGAAGGAAAATGTGGCATCTGCAACCGTTTTCACTCTGCTACTTTTTCTCAACACCTGCCTTCTGAAT; GTTCAGCCAGACCCTCCTTTGGAGCTGGCTGTGGAAGTAAAACAGCCAGAAGACAGAAAACCCTACCTG; TGGATTAAATGGTCTCCACCTACCCTGATTGACTTAAAAACTGGTTGGTTCACGCTCCTGTATGAAATT; CGATTAAAACCCGAGAAAGCAGCTGAGTGGGAGATCCATTTTGCTGGGCAGCAAACAGAGTTTAAGATT; CTCAGCCTACATCCAGGACAGAAATACCTTGTCCAGGTTCGCTGCAAACCAGACCATGGATACTGGAGT; GCATGGAGTCCAGCGACCTTCATTCAGATACCTAGTGACTTCACCATGAATGATACAACCGTGTGGATC; TCTGTGGCTGTCCTTTCTGCTGTCATCTGTTTGATTATTGTCTGGGCAGTGGCTTTGAAGGGCTATAGC; ATGGTGACCTGCATCTTTCCGCCAGTTCCTGGGCCAAAAATAAAAGGATTTGATGCTCATCTGTTGGAG; AAGGGCAAGTCTGAAGAACTACTGAGTGCCTTGGGATGCCAAGACTTTCCTCCCACTTCTGACTATGAG; GACTTGCTGGTGGAGTATTTAGAAGTAGATGATAGTGAGGACCAGCATCTAATGTCAGTCCATTCAAAA; GAACACCCAAGTCAAGGTATGAAACCCACATACCTGGATCCTGACACTGACTCAGGCCGGGGGAGCTGT; GACAGCCCTTCCCTTTTGTCTGAAAAGTGTGAGGAACCCCAGGCCAATCCCTCCACATTCTATGATCCT; GAGGTCATTGAGAAGCCAGAGAATCCTGAAACAACCCACACCTGGGACCCCCAGTGCATAAGCATGGAA; GGCAAAATCCCCTATTTTCATGCTGGTGGATCCAAATGTTCAACATGGCCCTTACCACAGCCCAGCCAG; CACAACCCCAGATCCTCTTACCACAATATTACTGATGTGTGTGAGCTGGCTGTGGGCCCTGCAGGTGCA; CCGGCCACTCTGTTGAATGAAGCAGGTAAAGATGCTTTAAAATCCTCTCAAACCATTAAGTCTAGAGAA; GAGGGAAAGGCAACCCAGCAGAGGGAGGTAGAAAGCTTCCATTCTGAGACTGACCAGGATACGCCCTGG; CTGCTGCCCCAGGAGAAAACCCCCTTTGGCTCCGCTAAACCCTTGGATTATGTGGAGATTCACAAGGTC; AACAAAGATGGTGCATTATCATTGCTACCAAAACAGAGAGAGAACAGCGGCAAGCCCAAGAAGCCCGGG; ACTCCTGAGAACAATAAGGAGTATGCCAAGGTGTCCGGGGTCATGGATAACAACATCCTGGTGTTGGTG; CCAGATCCACATGCTAAAAACGTGGCTTGCTTTGAAGAATCAGCCAAAGAGGCCCCACCATCACTTGAA; CAGAATCAAGCTGAGAAAGCCCTGGCCAACTTCACTGCAACATCAAGCAAGTGCAGGCTCCAGCTGGGT; GGTTTGGATTACCTGGATCCCGCATGTTTTACACACTCCTTTCACTGA;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001204314
• Insert Size: 1566 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001204314.1
• RefSeq Size: 11066 bp
• RefSeq ORF: 1566 bp
• Locus ID: 5618
• UniProt ID: P16471
• Cytogenetics: 5p13.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, Neuroactive ligand-receptor interaction
• MW: 58 kDa
• Summary: This gene encodes a receptor for the anterior pituitary hormone, prolactin, and belongs to the type I cytokine receptor family. Prolactin-dependent signaling occurs as the result of ligand-induced dimerization of the prolactin receptor. Several alternatively spliced transcript variants encoding different membrane-bound and soluble isoforms have been described for this gene, which may function to modulate the endocrine and autocrine effects of prolactin in normal tissue and cancer. [provided by RefSeq, Feb 2011]; Transcript Variant: This variant (6) lacks two in-frame coding exons compared to variant 1. This results in a shorter isoform (6, also known as deltaS1) missing an internal protein segment coding for the S1 motif, compared to isoform 1. This isoform has been shown to be expressed at the protein level on the cell surface, and is functional (PMID:12351696). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.