KIAA0652 (ATG13) (NM_001205122) Human Untagged Clone
Product Data | |
Type | Human Untagged Clone |
---|---|
Target Symbol | KIAA0652 |
Synonyms | KIAA0652; PARATARG8 |
Vector | pCMV6-Entry |
Sequence Data |
Fully Sequenced ORF
>SC329788 representing NM_001205122.
Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTGTGTGGAGATTTCACTTAAGACTTCTGAGGGAGATTCCATGGAGCTGGAAATATGGTGTCTTGAA ATGAATGAAAAGTGTGATAAAGAAATCAAAGTTTCCTACACGGTGTACAACAGACTGTCATTGCTGCTG AAGTCCCTTCTTGCTATAACTAGGGTGACACCAGCCTATAGGCTCTCCAGGAAACAAGGGCATGAATAT GTCATATTATACAGGATATATTTTGGAGAAGTTCAGCTGAGTGGCTTAGGAGAAGGCTTCCAGACAGTT CGTGTTGGGACAGTGGGCACCCCTGTGGGCACCATCACTCTTTCTTGTGCTTACAGAATTAACTTGGCA TTCATGTCTACCAGGCAATTTGAGAGGACCCCACCTATCATGGGGATTATTATTGATCACTTTGTGGAC CGTCCCTATCCCAGCTCCTCTCCCATGCACCCCTGCAATTACAGAACTGCTGGTGAGGACACTGGAGTA ATATACCCGTCTGTAGAAGACTCTCAAGAAGTGTGTACCACCTCTTTTTCCACCTCCCCACCATCCCAG CTGATGGTTCCTGGGAAGGAAGGTGGGGTACCCCTTGCTCCCAACCAGCCTGTCCATGGTACCCAGGCT GACCAGGAGAGACTGGCAACCTGCACCCCTTCTGACAGAACCCACTGTGCTGCCACACCCTCCAGTAGT GAGGATACTGAAACCGTATCAAACAGCAGTGAGGGACGGGCCTCCCCTCACGATGTCTTGGAGACCATC TTTGTCCGAAAAGTGGGGGCTTTTGTCAACAAACCCATTAACCAGGTGACCCTGACGAGTTTGGATATA CCCTTTGCCATGTTTGCTCCCAAGAATTTGGAGCTGGAGGATACCGATCCAATGGTGAATCCTCCAGAT TCCCCAGAGACTGAATCTCCTCTCCAGGGCAGCCTGCACTCAGATGGCTCCAGCGGGGGCAGCAGTGGC AATACCCATGATGACTTTGTTATGATAGACTTTAAACCAGCTTTTTCTAAAGATGACATTCTTCCGATG GACCTGGGGACCTTCTATCGGGAGTTTCAGAACCCACCTCAGCTGAGCAGCCTCTCCATAGATATTGGA GCACAGTCCATGGCTGAAGACTTGGACTCATTACCAGAGAAGCTGGCTGTGCATGAGAAGAATGTCCGC GAGTTTGATGCCTTTGTGGAAACCCTGCAGTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001205122 |
Insert Size | 1206 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Shipping | Ambient |
Reference Data | |
RefSeq | NM_001205122.1 |
RefSeq Size | 5674 bp |
RefSeq ORF | 1206 bp |
Locus ID | 9776 |
UniProt ID | O75143 |
Cytogenetics | 11p11.2 |
MW | 44.1 kDa |
Summary | The protein encoded by this gene is an autophagy factor and a target of the TOR kinase signaling pathway. The encoded protein is essential for autophagosome formation and mitophagy. [provided by RefSeq, Oct 2016] Transcript Variant: This variant (6) has multiple differences compared to variant 3. These differences result in a distinct 5' UTR and cause translation initiation at a downstream start codon compared to variant 3. The encoded isoform (h, also known as isoform 4) has a distinct N-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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