CRY2 (NM_021117) Human Untagged Clone

CAT#: SC327944

CRY2 (untagged)-Human cryptochrome 2 (photolyase-like) (CRY2) transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CRY2
• Synonyms: HCRY2; PHLL2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC327944 representing NM_021117.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGCGGGGTCCACGTCGCCTACCGGGGCGGAGCGGGGGTGGCTGGAGCAGTCTGGACAGTCATGGCG
  GCGACTGTGGCGACGGCGGCAGCTGTGGCCCCGGCGCCAGCGCCCGGCACGGACAGCGCCTCTTCGGTG
  CACTGGTTCCGCAAAGGGCTGCGACTCCACGACAACCCGGCGTTGCTGGCGGCCGTGCGCGGGGCGCGC
  TGCGTGCGCTGCGTTTACATTCTCGACCCGTGGTTCGCGGCCTCCTCCTCAGTCGGGATCAACCGATGG
  AGGTTCCTACTTCAGTCTCTGGAAGATTTGGACACAAGTTTAAGGAAACTGAACTCCCGCCTGTTTGTA
  GTCCGGGGACAGCCAGCCGACGTGTTCCCAAGGCTGTTCAAGGAATGGGGAGTGACCCGCTTGACCTTT
  GAATATGACTCTGAACCCTTTGGGAAAGAACGGGATGCAGCCATCATGAAGATGGCCAAGGAGGCTGGT
  GTGGAAGTAGTGACGGAGAATTCTCATACCCTCTATGACCTGGACAGGATCATTGAGCTGAATGGGCAG
  AAGCCACCCCTTACATACAAGCGCTTTCAGGCCATCATCAGCCGCATGGAGCTGCCCAAGAAGCCAGTG
  GGCTTGGTGACCAGCCAGCAGATGGAGAGCTGCAGGGCCGAGATCCAGGAGAACCACGACGAGACCTAC
  GGCGTGCCCTCCCTGGAGGAGCTGGGGTTCCCCACTGAAGGACTTGGTCCAGCTGTCTGGCAGGGAGGA
  GAGACAGAAGCTCTGGCCCGCCTGGATAAGCACTTGGAACGGAAGGCCTGGGTTGCCAACTATGAGAGA
  CCCCGAATGAACGCCAACTCCCTCCTGGCCAGCCCCACAGGCCTCAGCCCCTACCTGCGCTTTGGTTGT
  CTCTCCTGCCGCCTCTTCTACTACCGCCTGTGGGACCTGTATAAAAAGGTGAAGCGGAACAGCACACCT
  CCCCTCTCCCTATTTGGGCAACTCCTATGGCGAGAGTTCTTCTACACGGCAGCTACCAACAACCCCAGG
  TTTGACCGCATGGAGGGGAACCCCATCTGCATCCAGATCCCCTGGGACCGCAATCCTGAGGCCCTGGCC
  AAGTGGGCTGAGGGCAAGACAGGCTTCCCTTGGATTGATGCCATCATGACCCAACTGAGGCAGGAGGGC
  TGGATCCACCACCTGGCCCGGCATGCCGTGGCCTGCTTCCTGACCCGCGGGGACCTCTGGGTCAGCTGG
  GAGAGCGGGGTCCGGGTATTTGATGAGCTGCTCCTGGATGCAGATTTCAGCGTGAACGCAGGCAGCTGG
  ATGTGGCTGTCCTGCAGTGCTTTCTTCCAGCAGTTCTTCCACTGCTACTGCCCTGTGGGCTTTGGCCGT
  CGCACGGACCCCAGTGGGGACTACATCAGGCGATACCTGCCCAAATTGAAAGCGTTCCCCTCTCGATAC
  ATCTATGAGCCCTGGAATGCCCCAGAGTCAATTCAGAAGGCAGCCAAGTGCATCATTGGTGTGGACTAC
  CCACGGCCCATCGTCAACCATGCCGAGACCAGCCGGCTTAACATTGAACGAATGAAGCAGATTTACCAG
  CAGCTTTCGCGCTACCGGGGACTCTGTCTACTGGCATCTGTCCCTTCCTGTGTGGAAGACCTCAGTCAC
  CCTGTGGCAGAGCCCAGCTCGAGCCAGGCTGGCAGCATGAGCAGTGCAGGCCCAAGACCACTACCCAGT
  GGCCCAGCATCCCCCAAACGCAAGCTGGAAGCAGCCGAGGAACCACCTGGTGAAGAACTCAGCAAACGG
  GCCCGGGTGGCAGAGTTGCCAACCCCAGAGCTGCCGAGCAAGGATGCCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_021117
• Insert Size: 1845 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021117.3
• RefSeq Size: 4204 bp
• RefSeq ORF: 1845 bp
• Locus ID: 1408
• UniProt ID: Q49AN0
• Cytogenetics: 11p11.2
• Protein Families: Druggable Genome
• Protein Pathways: Circadian rhythm - mammal
• MW: 68.9 kDa
• Gene Summary: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (1) uses an alternate first exon which contains the predicted translation start site; the resulting protein has a distinct N-terminus. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is more supported by conservation.