HIC5 (TGFB1I1) (NM_001164719) Human Untagged Clone

SKU SC327534

TGFB1I1 (untagged)-Human transforming growth factor beta 1 induced transcript 1 (TGFB1I1) transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: HIC5
• Synonyms: ARA55; HIC-5; HIC5; TSC-5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC327534 representing NM_001164719.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGCCAAGGTCAGGGGCTCCCAAAGAGCGCCCTGCGGAGCCTCTCACCCCTCCCCCATCCTATGGCCAC; CAGCCACAGACAGGGTCTGGGGAGTCTTCAGGAGCCTCGGGGGACAAGGACCACCTGTACAGCACGGTA; TGCAAGCCTCGGTCCCCAAAGCCTGCAGCCCCGGCGGCCCCTCCATTCTCCTCTTCCAGCGGTGTCTTG; GGTACCGGGCTCTGTGAGCTAGATCGGTTGCTTCAGGAACTTAATGCCACTCAGTTCAACATCACAGAT; GAAATCATGTCTCAGTTCCCATCTAGCAAGGTGGCTTCAGGAGAGCAGAAGGAGGACCAGTCTGAAGAT; AAGAAAAGACCCAGCCTCCCTTCCAGCCCGTCTCCTGGCCTCCCAAAGGCTTCTGCCACCTCAGCCACT; CTGGAGCTGGATAGACTGATGGCCTCACTCTCTGACTTCCGCGTTCAAAACCATCTTCCAGCCTCTGGG; CCAACTCAGCCACCGGTGGTGAGCTCCACAAATGAGGGCTCCCCATCCCCACCAGAGCCGACTGGCAAG; GGCAGCCTAGACACCATGCTGGGGCTGCTGCAGTCCGACCTCAGCCGCCGGGGTGTTCCCACCCAGGCC; AAAGGCCTCTGTGGCTCCTGCAATAAACCTATTGCTGGGCAAGTGGTGACGGCTCTGGGCCGCGCCTGG; CACCCCGAGCACTTCGTTTGCGGAGGCTGTTCCACCGCCCTGGGAGGCAGCAGCTTCTTCGAGAAGGAT; GGAGCCCCCTTCTGCCCCGAGTGCTACTTTGAGCGCTTCTCGCCAAGATGTGGCTTCTGCAACCAGCCC; ATCCGACACAAGATGGTGACCGCCTTGGGCACTCACTGGCACCCAGAGCATTTCTGCTGCGTCAGTTGC; GGGGAGCCCTTCGGAGATGAGGGTTTCCACGAGCGCGAGGGCCGCCCCTACTGCCGCCGGGACTTCCTG; CAGCTGTTCGCCCCGCGCTGCCAGGGCTGCCAGGGCCCCATCCTGGATAACTACATCTCGGCGCTCAGC; GCGCTCTGGCACCCGGACTGTTTCGTCTGCAGGGAATGCTTCGCGCCCTTCTCGGGAGGCAGCTTTTTC; GAGCACGAGGGCCGCCCGTTGTGCGAGAACCACTTCCACGCACGACGCGGCTCGCTGTGCGCCACGTGT; GGCCTCCCTGTGACCGGCCGCTGCGTGTCGGCCCTGGGTCGCCGCTTCCACCCGGACCACTTCACATGC; ACCTTCTGCCTGCGCCCGCTCACCAAGGGGTCCTTCCAGGAGCGCGCCGGCAAGCCCTACTGCCAGCCC; TGCTTCCTGAAGCTCTTCGGCTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164719
• Insert Size: 1335 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001164719.1
• RefSeq Size: 1782 bp
• RefSeq ORF: 1335 bp
• Locus ID: 7041
• UniProt ID: O43294
• Cytogenetics: 16p11.2
• Protein Families: Druggable Genome, Transcription Factors
• MW: 47.9 kDa
• Summary: This gene encodes a coactivator of the androgen receptor, a transcription factor which is activated by androgen and has a key role in male sexual differentiation. The encoded protein is thought to regulate androgen receptor activity and may have a role to play in the treatment of prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]; Transcript Variant: This variant (3) differs in the 5' UTR and coding region, and initiates translation at an alternate start codon compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Variants 2 and 3 encode the same protein.