TCP1 eta (CCT7) (NM_001166284) Human Untagged Clone

SKU SC327006

CCT7 (untagged)-Human chaperonin containing TCP1 subunit 7 (eta) (CCT7) transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: TCP1 eta
• Synonyms: CCTETA; CCTH; NIP7-1; TCP1ETA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC327006 representing NM_001166284.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGATGGTGGGTGATGGCACCACCTCAGTGACCTTGCTGGCTGCAGAGTTTCTGAAGCAGGTGAAACCC; TATGTGGAGGAAGGTTTACACCCCCAGATCATCATTCGAGCTTTCCGCACAGCCACCCAGCTGGCAGTT; AACAAGATCAAAGAGATTGCTGTGACCGTGAAGAAGGCAGATAAAGTGGAGCAGAGGAAGCTGCTGGAA; AAGTGTGCCATGACCGCTCTGAGCTCCAAGCTGATCTCCCAGCAGAAAGCTTTCTTTGCTAAGATGGTG; GTGGATGCAGTGATGATGCTCGATGATTTGCTGCAGCTTAAAATGATTGGAATCAAGAAGGTACAGGGT; GGAGCCCTCGAGGATTCTCAGCTGGTAGCTGGTGTTGCATTCAAGAAGACTTTCTCTTACGCTGGGTTT; GAAATGCAACCCAAAAAGTACCACAATCCCAAGATTGCCCTTTTGAATGTCGAGCTCGAGTTGAAAGCT; GAGAAAGACAATGCTGAGATAAGAGTCCACACAGTTGAGGATTATCAGGCAATTGTTGATGCTGAGTGG; AACATTCTCTATGACAAGTTAGAGAAGATCCATCATTCTGGAGCCAAAGTTGTCTTGTCCAAACTCCCC; ATTGGGGATGTGGCCACCCAGTACTTTGCTGACAGGGACATGTTCTGTGCTGGCCGAGTACCTGAGGAG; GATCTGAAGAGGACAATGATGGCCTGTGGAGGCTCAATCCAGACCAGTGTGAATGCTCTGTCAGCAGAT; GTGCTGGGTCGATGCCAGGTGTTTGAAGAGACCCAGATTGGAGGCGAGAGGTACAATTTTTTTACTGGC; TGCCCCAAGGCCAAGACATGCACCTTCATTCTCCGTGGCGGCGCCGAGCAGTTTATGGAGGAGACAGAG; CGGTCCCTGCATGATGCCATCATGATCGTCAGGAGGGCCATCAAGAATGATTCAGTGGTGGCTGGTGGC; GGGGCCATTGAGATGGAACTCTCCAAGTACCTGCGGGATTACTCAAGGACTATTCCAGGAAAACAGCAG; CTGTTGATTGGGGCATATGCCAAGGCCTTGGAGATTATCCCACGCCAGCTGTGTGACAATGCTGGCTTT; GATGCCACAAACATTCTCAACAAGCTGCGGGCTCGGCATGCCCAGGGGGGTACATGGTATGGAGTAGAC; ATCAACAACGAGGACATTGCTGACAACTTTGAAGCTTTCGTGTGGGAGCCAGCTATGGTGCGGATCAAT; GCGCTGACAGCAGCCTCTGAGGCTGCGTGCCTGATCGTGTCTGTAGATGAAACCATCAAGAACCCCCGC; TCGACTGTGGATGCTCCCACAGCAGCAGGCCGGGGCCGTGGTCGTGGCCGCCCCCACTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001166284
• Insert Size: 1371 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001166284.1
• RefSeq Size: 1687 bp
• RefSeq ORF: 1371 bp
• Locus ID: 10574
• UniProt ID: Q99832
• Cytogenetics: 2p13.2
• MW: 50.4 kDa
• Summary: This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 6. [provided by RefSeq, Oct 2009]; Transcript Variant: This variant (3) lacks an alternate in-frame segment of the 5' coding region, compared to variant 1, resulting in a shorter isoform (c), compared to isoform a.