ANO6 (NM_001142680) Human Untagged Clone

SKU SC326290

ANO6 (untagged)-Human anoctamin 6 (ANO6), transcript variant 4

Product Data

• Type: Human Untagged Clone
• Target Symbol: ANO6
• Synonyms: MGC104751; TMEM16F
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC326290 representing NM_001142680.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGAAAAAGATGAGCAGGAATGTTTTGCTACAAATGGAGGAGGAGGAGGACGACGACGATGGGGATATC; GTGTTGGAAAACCTTGGACAGACAATTGTCCCCGATTTGGGATCACTGGAAAGTCAGCATGATTTTCGA; ACCCCGGAGTTTGAAGAATTTAATGGAAAACCTGACTCCCTCTTTTTTAATGATGGCCAGCGAAGAATT; GACTTTGTTCTAGTATATGAGGATGAAAGCAGAAAAGAGACCAATAAAAAGGGTACAAATGAAAAACAA; AGGAGGAAAAGACAAGCATACGAATCTAACCTTATCTGTCATGGCCTGCAGTTAGAAGCAACAAGATCA; GTATTGGATGACAAGCTTGTATTTGTAAAAGTACACGCACCATGGGAGGTGTTATGTACGTATGCTGAG; ATAATGCACATCAAATTGCCTCTGAAACCCAATGATCTGAAAAACCGGTCCTCAGCCTTTGGTACACTC; AACTGGTTTACCAAAGTCCTCAGTGTAGACGAAAGCATCATCAAGCCAGAGCAAGAGTTTTTCACTGCC; CCATTTGAGAAGAACCGGATGAATGATTTTTACATAGTTGATAGAGATGCTTTCTTCAATCCAGCCACC; AGAAGCCGCATTGTTTACTTCATCCTCTCTCGGGTCAAGTATCAAGTGATAAACAATGTTAGCAAGTTT; GGGATCAACAGACTTGTAAACTCTGGGATCTACAAGGCAGCTTTCCCACTCCATGATTGCAAATTCCGC; CGTCAGTCAGAGGATCCCAGCTGCCCTAATGAACGGTACCTTCTGTACAGAGAATGGGCTCATCCTCGA; AGCATATACAAAAAGCAGCCCTTGGATCTTATCAGGAAATACTATGGAGAGAAGATTGGAATCTACTTT; GCTTGGCTGGGCTATTACACTCAGATGCTTCTCCTGGCCGCAGTTGTAGGAGTGGCTTGCTTTCTCTAT; GGATATCTTAATCAAGATAACTGTACATGGAGCAAAGAAGTTTGTCATCCTGATATTGGTGGCAAGATC; ATAATGTGTCCTCAGTGTGATAGGCTTTGTCCATTCTGGAAACTCAATATTACTTGCGAGTCCTCAAAG; AAATTGTGCATCTTCGACAGTTTTGGAACCCTGGTCTTTGCAGTATTTATGGGAGTATGGGTTACCTTG; TTTTTGGAGTTTTGGAAGCGACGCCAGGCAGAACTTGAGTATGAATGGGATACTGTTGAGTTACAGCAG; GAAGAACAAGCCCGACCAGAATACGAAGCACGATGTACTCACGTAGTGATAAATGAGATTACTCAGGAA; GAAGAACGCATTCCCTTTACTGCCTGGGGAAAATGTATACGGATAACCCTCTGTGCCAGTGCTGTCTTT; TTCTGGATCCTATTGATCATCGCTTCAGTTATTGGGATCATTGTCTATAGGCTCTCGGTGTTCATTGTA; TTTTCTGCAAAACTTCCCAAGAACATTAATGGAACAGACCCAATCCAGAAATACCTGACTCCACAGACA; GCCACGTCCATCACGGCCTCCATCATCAGCTTTATAATTATCATGATTCTGAACACCATATATGAAAAA; GTGGCAATTATGATTACTAACTTCGAACTCCCAAGGACCCAGACTGATTATGAGAACAGCCTCACCATG; AAGATGTTCTTATTCCAGTTTGTCAACTACTACTCTTCATGCTTCTACATAGCATTCTTTAAGGGCAAA; TTTGTAGGCTATCCAGGAGACCCAGTTTATTGGTTGGGAAAATACAGAAATGAAGAGTGTGACCCAGGT; GGCTGTCTTCTTGAACTGACAACTCAGCTGACAATAATCATGGGAGGAAAAGCAATCTGGAATAACATA; CAAGAAGTATTATTGCCCTGGATCATGAATCTAATTGGGCGATTTCACAGAGTTTCTGGATCAGAAAAG; ATAACCCCACGATGGGAACAGGACTACCATCTGCAGCCTATGGGCAAACTGGGATTATTTTATGAATAT; CTTGAAATGATTATTCAGTTTGGGTTCGTCACCTTATTTGTGGCCTCTTTTCCACTGGCCCCTCTGTTG; GCTCTCGTGAACAATATATTGGAAATAAGAGTGGACGCATGGAAACTGACCACCCAGTTTAGACGCCTG; GTACCAGAGAAAGCCCAAGACATTGGAGCATGGCAGCCCATCATGCAAGGAATAGCAATTCTGGCTGTG; GTGACCAATGCCATGATCATAGCTTTCACGTCGGACATGATCCCCCGCCTAGTGTACTACTGGTCCTTC; TCCGTCCCTCCCTACGGGGACCACACTTCCTACACCATGGAAGGGTACATCAACAACACTCTCTCCATC; TTCAAAGTCGCAGACTTCAAAAACAAAAGCAAGGGAAACCCGTACTCTGACCTGGGTAACCATACCACA; TGCAGGTATCGTGATTTCCGATACCCACCTGGACACCCCCAGGAGTATAAACACAACATCTACTATTGG; CATGTGATTGCAGCCAAGCTGGCTTTTATCATTGTCATGGAGTATCTCGCTTTGTTGCCCAGGCTGGGA; CACAGTGGCATGATCTTGGCTCACTGCAACCTCCGCCTCCCAGTTGACTGCTGTATGTGTTATAGGTTT; GTCGATGAAATTAGACTTTTGGAACAACTAACTTCTGACTTTATTGACTCCCTCTATTATATATTTTCT; ATTTCAATAATATCTATTTTCTTTTCTGTTACTTTCTTTTTCCTTCTACTTTCTTTGGGGCCAACTCCG; TGTTTTTCTGTATCGAATTTCTTAAGTTAG; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001142680
• Insert Size: 2790 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001142680.1
• RefSeq Size: 3448 bp
• RefSeq ORF: 2790 bp
• Locus ID: 196527
• Cytogenetics: 12q12
• Protein Families: Transmembrane
• MW: 108.1 kDa
• Summary: This gene encodes a multi-pass transmembrane protein that belongs to the anoctamin family. This protein is an essential component for the calcium-dependent exposure of phosphatidylserine on the cell surface. The scrambling of phospholipid occurs in various biological systems, such as when blood platelets are activated, they expose phosphatidylserine to trigger the clotting system. Mutations in this gene are associated with Scott syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]; Transcript Variant: This variant (4) differs in the 5' UTR and contains an alternate 3' terminal exon compared to variant 1, resulting in an isoform (c) with a longer and distinct C-terminus compared to isoform a. Variants 3 and 4 encode the same isoform.