HEF1 (NEDD9) (NM_001142393) Human Untagged Clone

SKU SC326249

NEDD9 (untagged)-Human neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: HEF1
• Synonyms: CAS-L; CAS2; CASL; CASS2; HEF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC326249 representing NM_001142393.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGTGGACAAGGAATCTTATGGCAAGGGCCTTATATGACAATGTCCCAGAGTGTGCCGAGGAACTGGCC; TTTCGCAAGGGAGACATCCTGACCGTCATAGAGCAGAACACAGGGGGACTGGAAGGATGGTGGCTGTGC; TCATTACACGGTCGGCAAGGCATTGTCCCAGGCAACCGGGTGAAGCTTCTGATTGGTCCCATGCAGGAG; ACTGCCTCCAGTCACGAGCAGCCTGCCTCTGGACTGATGCAGCAGACCTTTGGCCAACAGAAGCTCTAT; CAAGTGCCAAACCCACAGGCTGCTCCCCGAGACACCATCTACCAAGTGCCACCTTCCTACCAAAATCAG; GGAATTTACCAAGTCCCCACTGGCCACGGCACCCAAGAACAAGAGGTATATCAGGTGCCACCATCAGTG; CAGAGAAGCATTGGGGGAACCAGTGGGCCCCACGTGGGTAAAAAGGTGATAACCCCCGTGAGGACAGGC; CATGGCTACGTATACGAGTACCCATCCAGATACCAAAAGGACGTCTATGATATCCCTCCTTCTCATACC; ACTCAAGGGGTATACGACATCCCTCCCTCATCAGCAAAAGGCCCTGTGTTTTCAGTTCCAGTGGGAGAG; ATAAAACCTCAAGGGGTGTATGACATCCCGCCTACAAAAGGGGTATATGCCATTCCGCCCTCTGCTTGC; CGGGATGAAGCAGGGCTTAGGGAAAAAGACTATGACTTCCCCCCTCCCATGAGACAAGCTGGAAGGCCG; GACCTCAGACCGGAGGGGGTTTATGACATTCCTCCAACCTGCACCAAGCCAGCAGGGAAGGACCTTCAT; GTAAAATACAACTGTGACATTCCAGGAGCTGCAGAACCGGTGGCTCGAAGGCACCAGAGCCTGTCCCCG; AATCACCCACCCCCGCAACTCGGACAGTCAGTGGGCTCTCAGAACGACGCATATGATGTCCCCCGAGGC; GTTCAGTTTCTTGAGCCACCAGCAGAAACCAGTGAGAAAGCAAACCCCCAGGAAAGGGATGGTGTTTAT; GATGTCCCTCTGCATAACCCGCCAGATGCTAAAGGCTCTCGGGACTTGGTGGATGGGATCAACCGATTG; TCTTTCTCCAGTACAGGCAGCACCCGGAGTAACATGTCCACGTCTTCCACCTCCTCCAAGGAGTCCTCA; CTGTCAGCCTCCCCAGCTCAGGACAAAAGGCTCTTCCTGGATCCAGACACAGCTATTGAGAGACTTCAG; CGGCTCCAGCAGGCCCTTGAGATGGGTGTCTCCAGCCTAATGGCACTGGTCACTACCGACTGGCGGTGT; TACGGATATATGGAAAGACACATCAATGAAATACGCACAGCAGTGGACAAGGTGGAGCTGTTCCTGAAG; GAGTACCTCCACTTTGTCAAGGGAGCTGTTGCAAATGCTGCCTGCCTCCCGGAACTCATCCTCCACAAC; AAGATGAAGCGGGAGCTGCAACGAGTTGAAGACTCCCACCAGATCCTGAGTCAAACCAGCCATGACTTA; AATGAGTGCAGCTGGTCCCTGAATATCTTGGCCATCAACAAGCCCCAGAACAAGTGTGACGATCTGGAC; CGGTTTGTGATGGTGGCAAAGACGGTGCCCGATGACGCCAAGCAGCTCACCACAACCATCAACACCAAC; GCAGAGGCCCTCTTCAGACCCGGCCCTGGCAGCTTGCATCTGAAGAATGGGCCGGAGAGCATCATGAAC; TCAACGGAGTACCCACACGGTGGCTCCCAGGGACAGCTGCTGCATCCTGGTGACCACAAGGCCCAGGCC; CACAACAAGGCACTGCCCCCAGGCCTGAGCAAGGAGCAGGCCCCTGACTGTAGCAGCAGTGATGGTTCT; GAGAGGAGCTGGATGGATGACTACGATTACGTCCACCTACAGGGTAAGGAGGAGTTTGAGAGGCAACAG; AAAGAGCTATTGGAAAAAGAGAATATCATGAAACAGAACAAGATGCAGCTGGAACATCATCAGCTGAGC; CAGTTCCAGCTGTTGGAACAAGAGATTACAAAGCCCGTGGAGAATGACATCTCGAAGTGGAAGCCCTCT; CAGAGCCTACCCACCACAAACAGTGGCGTGAGTGCTCAGGATCGGCAGTTGCTGTGCTTCTACTATGAC; CAATGTGAGACCCATTTCATTTCCCTTCTCAACGCCATTGACGCACTCTTCAGTTGTGTCAGCTCAGCC; CAGCCCCCGCGAATCTTCGTGGCACACAGCAAGTTTGTCATCCTCAGTGCACACAAACTGGTGTTCATT; GGAGACACGCTGACACGGCAGGTGACTGCCCAGGACATTCGCAACAAAGTCATGAACTCCAGCAACCAG; CTCTGCGAGCAGCTCAAGACCATAGTCATGGCAACCAAGATGGCCGCCCTCCATTACCCCAGCACCACG; GCCCTGCAGGAAATGGTGCACCAAGTGACAGACCTTTCTAGAAATGCCCAGCTGTTCAAGCGCTCTTTG; CTGGAGATGGCAACGTTCTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001142393
• Insert Size: 2505 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001142393.1
• RefSeq Size: 4745 bp
• RefSeq ORF: 2505 bp
• Locus ID: 4739
• UniProt ID: Q14511
• Cytogenetics: 6p24.2
• MW: 92.9 kDa
• Summary: The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]; Transcript Variant: This variant (3) contains an alternate 5' UTR and coding exon and initiates translation at an upstream AUG compared to variant 1. The resulting isoform (3) has a distinct N-terminus, compared to isoform 1.