beta COP (COPB1) (NM_001144061) Human Untagged Clone

SKU SC325377

COPB1 (untagged)-Human coatomer protein complex, subunit beta 1 (COPB1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: beta COP
• Synonyms: BARMACS; COPB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC325377 representing NM_001144061.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGACGGCGGCTGAGAACGTATGCTACACGTTAATTAACGTGCCAATGGATTCAGAACCACCATCTGAA; ATTAGCTTAAAAAATGATCTAGAAAAAGGAGATGTAAAGTCAAAGACTGAAGCTTTGAAGAAAGTAATC; ATTATGATTCTGAATGGTGAAAAACTTCCTGGACTTCTGATGACCATCATTCGTTTTGTGCTACCTCTT; CAGGATCACACTATCAAGAAATTACTTCTGGTATTTTGGGAAATTGTTCCTAAAACAACTCCAGATGGG; AGACTTTTACATGAGATGATCCTTGTATGTGATGCATACAGAAAGGATCTTCAACATCCTAATGAATTT; ATTCGAGGATCTACTCTTCGTTTTCTTTGCAAATTGAAAGAAGCAGAATTGCTAGAACCTTTAATGCCA; GCTATTCGTGCATGTTTGGAGCATCGACACAGCTATGTTAGAAGAAATGCTGTTTTGGCCATCTATACC; ATCTATAGAAATTTTGAACATCTTATACCTGATGCTCCTGAACTGATACATGATTTTCTGGTGAATGAG; AAGGATGCAAGTTGCAAAAGGAATGCATTTATGATGCTAATTCATGCAGATCAGGATCGAGCTTTGGAT; TACTTAAGTACTTGCATTGATCAAGTTCAAACATTTGGAGACATTCTGCAGCTGGTTATTGTTGAACTG; ATTTATAAGGTCTGTCATGCTAATCCATCAGAAAGAGCTCGTTTTATTCGCTGCATCTATAACTTATTA; CAGTCATCCAGCCCTGCTGTAAAATATGAAGCTGCTGGGACATTAGTGACACTCTCTAGTGCACCAACT; GCAATCAAGGCTGCTGCTCAGTGTTACATTGATTTAATTATTAAGGAGAGCGACAACAATGTAAAACTC; ATAGTTTTGGATCGCTTGATAGAATTAAAAGAGCATCCTGCTCATGAACGAGTACTACAGGATCTGGTT; ATGGATATCCTAAGAGTATTGAGCACACCAGACTTAGAAGTACGAAAGAAAACTCTGCAGTTAGCACTG; GATCTTGTCTCTTCTAGAAATGTTGAAGAGCTGGTTATTGTCCTGAAGAAGGAAGTGATAAAAACAAAT; AATGTGTCTGAGCATGAAGATACTGACAAATACAGACAACTCCTAGTGCGAACATTGCATTCCTGTTCT; GTCCGATTTCCAGATATGGCTGCAAATGTTATTCCTGTGTTAATGGAATTTCTCAGTGACAACAACGAA; GCAGCAGCTGCTGATGTCTTGGAGTTTGTTCGTGAAGCCATTCAGCGCTTTGATAACCTGAGAATGCTT; ATTGTTGAGAAGATGCTTGAAGTCTTTCATGCTATTAAATCTGTCAAGATTTACCGAGGAGCATTATGG; ATCCTGGGAGAATACTGTAGTACCAAGGAAGACATTCAGAGTGTGATGACTGAGATCCGCAGGTCCCTT; GGAGAGATCCCAATTGTAGAGTCAGAAATAAAGAAAGAAGCTGGTGAATTAAAACCTGAAGAAGAAATA; ACTGTAGGGCCAGTTCAGAAATTGGTTACTGAAATGGGTACCTATGCAACTCAGAGTGCCCTTAGCAGT; TCTAGACCCACCAAGAAAGAGGAAGACAGACCTCCCTTGAGAGGATTCCTTCTGGATGGAGATTTCTTT; GTTGCTGCCTCCCTTGCCACAACTCTGACCAAGATTGCATTGCGCTATGTAGCTTTGGTTCAGGAGAAG; AAAAAGCAAAATTCTTTTGTTGCTGAGGCTATGTTGCTCATGGCTACTATCCTGCATTTGGGAAAATCC; TCTCTTCCTAAGAAGCCAATTACTGATGATGATGTGGATCGAATTTCCCTGTGCCTCAAGGTCTTGTCT; GAATGTTCACCTTTAATGAATGACATTTTCAATAAGGAATGCAGACAGTCCCTTTCTCACATGTTATCT; GCTAAACTAGAAGAAGAGAAATTATCCCAAAAGAAAGAATCTGAAAAGAGGAATGTGACAGTACAGCCT; GATGACCCCATTTCCTTCATGCAACTAACTGCTAAGAATGAAATGAACTGCAAGGAAGATCAGTTTCAG; CTGAGTTTACTGGCAGCAATGGGTAACACACAGAGGAAAGAGGCAGCAGATCCCCTAGCATCTAAACTT; AACAAGGTCACCCAATTGACAGGTTTCTCAGATCCTGTATATGCAGAAGCTTACGTTCATGTCAACCAA; TATGATATTGTCCTGGATGTACTTGTTGTGAACCAAACCAGTGATACTTTGCAGAATTGCACATTAGAA; CTAGCTACACTAGGGGATCTGAAACTTGTGGAAAAGCCGTCTCCTTTGACTCTTGCTCCTCATGACTTC; GCAAATATTAAAGCTAACGTCAAAGTAGCATCAACAGAAAATGGAATAATTTTTGGTAATATAGTTTAT; GATGTCTCTGGAGCAGCAAGTGACAGAAATTGTGTGGTTCTCAGTGATATTCACATCGACATCATGGAC; TATATCCAGCCTGCAACTTGCACTGATGCAGAATTCCGTCAGATGTGGGCCGAATTTGAATGGGAAAAC; AAAGTGACAGTTAACACCAACATGGTTGATTTAAATGACTACTTACAGCACATATTAAAGTCAACCAAT; ATGAAATGCCTGACTCCAGAAAAGGCCCTTTCTGGTTACTGTGGCTTTATGGCAGCCAACCTTTATGCT; CGTTCCATATTTGGTGAAGATGCACTTGCAAATGTCAGCATTGAGAAGCCAATTCACCAGGGACCAGAT; GCTGCTGTTACCGGCCATATAAGAATTCGTGCAAAGAGCCAGGGAATGGCCTTAAGTCTTGGAGATAAA; ATCAACTTGTCACAGAAGAAAACTAGTATATAA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001144061
• Insert Size: 2862 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001144061.1
• RefSeq Size: 3429 bp
• RefSeq ORF: 2862 bp
• Locus ID: 1315
• UniProt ID: P53618
• Cytogenetics: 11p15.2
• MW: 107.1 kDa
• Summary: This gene encodes a protein subunit of the coatomer complex associated with non-clathrin coated vesicles. The coatomer complex, also known as the coat protein complex 1, forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases. Once at the Golgi membrane, the coatomer complex may assist in the movement of protein and lipid components back to the endoplasmic reticulum. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]; Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.