DDX4 (NM_001136034) Human Untagged Clone

SKU SC325303

DDX4 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4), transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: DDX4
• Synonyms: DEAD (Asp-Glu-Ala-Asp) box polypeptide 4; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4; MGC111074; OTTHUMP00000122546; VASA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC325303 representing NM_001136034.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGGAGATGAAGATTGGGAAGCAGAAATCAACCCTCATATGTCTTCCTATGTTCCCATATTTGAGAAG; GATAGGTATTCTGGAGAAAATGGAGACAATTTTAACAGGACTCCAGCTTCATCATCAGAAATGGATGAT; GGACCTTCTCGAAGAGATCATTTCATGAAAAGTGGATTTGCCTCTGGGCGGAATTTTGGAAACAGAGAT; GCTGGTGAGTGTAATAAGCGAGATAATACATCCACAATGGGTGGTTTTGGAGTTGGAAAGAGTTTTGGA; AACAGAGGTTTTTCAAACAGCAGGTTTGAAGATGGTGATAGCTCTGGTTTCTGGAGAGAGTCTAGTAAT; GACTGCGAAGATAATCCAACACGGAACAGAGGGTTTTCCAAGAGAGGCGGCTATCGAGATGGAAATAAT; TCAGAAGCTTCAGGGCCATACAGAAGAGGTGGAAGAGGTAGTTTCCGAGGTTGCCGTGGAGGATTTGGT; CTAGGAAGTCCAAATAATGACTTAGACCCAGACGAATGTATGCAGCGCACTGGTGGCCTTTTTGGTTCT; AGAAGACCAGTATTAAGTGGCACAGGTAATGGTGATACTTCTCAAAGCAGAAGTGGCAGTGGAAGTGAA; CGAGGTGGTTACAAAGGTTTAAATGAAGAAGTAATAACAGGCTCTGGAAAGAATTCTTGGAAGTCAGAA; GCAGAAGGAGGAGAAAGTAGTGATACTCAAGGACCAAAAGTGACCTACATACCCCCTCCTCCACCTGAG; GATGAGGACTCCATCTTTGCACATTATCAGACAGGCATAAACTTCGACAAATACGACACTATTCTTGTG; GAAGTGTCTGGACATGATGCACCACCAGCAATTCTGACTTTTGAAGAAGCTAATCTCTGTCAGACACTG; AATAACAACATTGCTAAAGCTGGTTATACTAAGCTTACTCCTGTGCAAAAATACAGTATTCCTATCATA; CTTGCAGGACGAGATTTGATGGCTTGCGCTCAAACAGGGTCTGGGAAGACTGCGGCTTTTCTCCTACCA; ATTTTGGCTCATATGATGCATGATGGAATAACTGCCAGTCGTTTTAAAGAGTTGCAGGAACCAGAGTGT; ATTATTGTAGCACCAACTCGAGAATTGGTCAACCAGATTTATTTGGAAGCCAGAAAATTTTCTTTTGGG; ACTTGTGTAAGAGCTGTTGTTATATATGGGGGAACCCAGCTGGGACATTCAATTCGACAAATAGTACAA; GGCTGTAATATATTATGTGCTACTCCTGGAAGACTGATGGATATCATAGGCAAAGAAAAGATTGGTCTC; AAACAGATCAAATACTTAGTTTTGGATGAAGCTGATCGCATGTTGGATATGGGTTTTGGTCCAGAAATG; AAGAAGTTAATTTCTTGCCCAGGAATGCCATCAAAGGAACAGCGCCAAACCCTTATGTTCAGTGCAACT; TTTCCAGAGGAAATTCAAAGGTTGGCTGCAGAGTTTTTAAAGTCAAATTATCTGTTTGTTGCTGTTGGA; CAAGTGGGTGGAGCATGTAGAGATGTTCAGCAGACCGTTCTCCAAGTTGGCCAGTTCTCAAAAAGAGAA; AAGCTCGTTGAAATTCTGCGAAACATAGGGGATGAAAGAACTATGGTCTTTGTTGAAACTAAGAAAAAA; GCAGATTTTATTGCAACTTTTCTTTGTCAAGAAAAAATATCAACTACAAGTATTCATGGTGATCGGGAA; CAGAGAGAGCGGGAGCAAGCTCTTGGAGATTTTCGCTTTGGAAAGTGCCCAGTTCTTGTTGCTACTTCA; GTAGCTGCCAGAGGGCTGGATATTGAAAATGTGCAACATGTTATCAATTTTGATCTTCCTTCTACCATT; GATGAATATGTTCATCGAATTGGGCGTACTGGTCGTTGTGGGAATACTGGCAGAGCAATTTCCTTTTTT; GATCTTGAATCGGATAACCATTTAGCACAGCCTCTAGTAAAAGTATTGACAGATGCTCAACAGGATGTT; CCTGCATGGTTGGAAGAAATTGCCTTTAGTACATACATTCCTGGCTTCAGTGGTAGTACAAGAGGAAAC; GTGTTTGCATCAGTTGATACCAGAAAGGGCAAGAGCACTTTGAACACAGCTGGGTTTTCTTCTTCACAA; GCTCCCAATCCAGTAGATGATGAGTCATGGGATTAA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001136034
• Insert Size: 2175 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001136034.1
• RefSeq Size: 2643 bp
• RefSeq ORF: 2175 bp
• Locus ID: 54514
• Cytogenetics: 5q11.2
• MW: 79.3 kDa
• Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]