SSB (NM_003142) Human Untagged Clone

CAT#: SC324522

SSB (untagged)-Human Sjogren syndrome antigen B (autoantigen La) (SSB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SSB
• Synonyms: La; La/SSB; LARP3
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003142.3 GGAGCGCTTTAGGCTGGCCGGCGGCGCTGGGAGGTGGAGTCGTTGCTGTTGCTGTTTGTG
  AGCCTGTGGCGCGGCTTCTGTGGGCCGGAACCTTAAAGATAGCCGCAATGGCTGAAAATG
  GTGATAATGAAAAGATGGCTGCCCTGGAGGCCAAAATCTGTCATCAAATTGAGTATTATT
  TTGGCGACTTCAATTTGCCACGGGACAAGTTTCTAAAGGAACAGATAAAACTGGATGAAG
  GCTGGGTACCTTTGGAGATAATGATAAAATTCAACAGGTTGAACCGTCTAACAACAGACT
  TTAATGTAATTGTGGAAGCATTGAGCAAATCCAAGGCAGAACTCATGGAAATCAGTGAAG
  ATAAAACTAAAATCAGAAGGTCTCCAAGCAAACCCCTACCTGAAGTGACTGATGAGTATA
  AAAATGATGTAAAAAACAGATCTGTTTATATTAAAGGCTTCCCAACTGATGCAACTCTTG
  ATGACATAAAAGAATGGTTAGAAGATAAAGGTCAAGTACTAAATATTCAGATGAGAAGAA
  CATTGCATAAAGCATTTAAGGGATCAATTTTTGTTGTGTTTGATAGCATTGAATCTGCTA
  AGAAATTTGTAGAGACCCCTGGCCAGAAGTACAAAGAAACAGACCTGCTAATACTTTTCA
  AGGACGATTACTTTGCCAAAAAAAATGAAGAAAGAAAACAAAATAAAGTGGAAGCTAAAT
  TAAGAGCTAAACAGGAGCAAGAAGCAAAACAAAAGTTAGAAGAAGATGCTGAAATGAAAT
  CTCTAGAAGAAAAGATTGGATGCTTGCTGAAATTTTCGGGTGATTTAGATGATCAGACCT
  GTAGAGAAGATTTACACATACTTTTCTCAAATCATGGTGAAATAAAATGGATAGACTTCG
  TCAGAGGAGCAAAAGAGGGGATAATTCTATTTAAAGAAAAAGCCAAGGAAGCATTGGGTA
  AAGCCAAAGATGCAAATAATGGTAACCTACAATTAAGGAACAAAGAAGTGACTTGGGAAG
  TACTAGAAGGAGAGGTGGAAAAAGAAGCACTGAAGAAAATAATAGAAGACCAACAAGAAT
  CCCTAAACAAATGGAAGTCAAAAGGTCGTAGATTTAAAGGAAAAGGAAAGGGTAATAAAG
  CTGCCCAGCCTGGGTCTGGTAAAGGAAAAGTACAGTTTCAGGGCAAGAAAACGAAATTTG
  CTAGTGATGATGAACATGATGAACATGATGAAAATGGTGCAACTGGACCTGTGAAAAGAG
  CAAGAGAAGAAACAGACAAAGAAGAACCTGCATCCAAACAACAGAAAACAGAAAATGGTG
  CTGGAGACCAGTAGTTTAGTAAACCAATTTTTTATTCATTTTAAATAGGTTTTAAACGAC
  TTTTGTTTGCGGGGCTTTTAAAAGGAAAACCGAATTAGGTCCACTTCAATGTCCACCTGT
  GAGAAAGGAAAAATTTTTTTGTTGTTTAACTTGTCTTTTTGTTATGCAAATGAGATTTCT
  TTGAATGTATTGTTCTGTTTGTGTTATTTCAGATGATTCAAATATCAAAAGGAAGATTCT
  TCCATTAAATTGCCTTTGTAATATGAGAATGTATTAGTACAAACTAACTAATAAAATATA
  TACTATATGAAAAGAGCAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_003142
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003142.3, NP_003133.1
• RefSeq Size: 1673 bp
• RefSeq ORF: 1227 bp
• Locus ID: 6741
• UniProt ID: P05455
• Cytogenetics: 2q31.1
• Domains: RRM, LA
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Systemic lupus erythematosus
• Gene Summary: The protein encoded by this gene is involved in diverse aspects of RNA metabolism, including binding and protecting poly(U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5' and 3' ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus. Alternative promoter usage results in two different transcript variants which encode the same protein. [provided by RefSeq, Jun 2014]
  Transcript Variant: This variant (1) and variant 2 encode the same protein.