Antizyme inhibitor 1 (AZIN1) (NM_148174) Human Untagged Clone

SKU SC324495

AZIN1 (untagged)-Human antizyme inhibitor 1 (AZIN1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: Antizyme inhibitor 1
• Synonyms: AZI; AZI1; AZIA1; OAZI; OAZIN; ODC1L
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_148174.2 ATCGCGGCCGCAGTTTTTCCTTTTTTCTTCTGCCGTCGCCTTCTCTGCCTCTTCTCATCC; TTTCTCGCTCTGCTGCTCTGCAGTGTGACGAGTCCGAATCCTCTTCCCACCCAGCCCGCG; CCTTTCTTCTTTTGCCTGCGCTGTTCTATTTCTCCTTCGGCCGCCGCCGCCACTGCTGCA; CACAGCTGGTGTCGGTGCCGCGCTTTTACCCCCAAGTCGTTCCCGCAGCCTATGGCCCAG; GCCGCCTTGGGTATTTCTGCTCAAGGTAACCACATCCCTCTTTAAAAATTCCGCCGAAAA; AGAGAAGACGCTTTACCCGACTCTTTGGGCCGTTATCTCACGGCGAACTTTCTGACCAAG; TATACAACTACCCAGAGGGCCTAGGAGAAGTGCTGTATAGAGAGCAGTTCGACTTCAACG; CTGAGCCACCTTGGGAACCTAGCTGATGATAGGGGGGTTCCATCTCCCAACTTGTCCATT; TTGTTGCATATTCTAAGGACCCAGACATAGGCTTGGTGGCCCGTCTCTTGTCTTTCCTGG; TTTATGACTTTCGGCTTTGTGGAATACGGCTGAGATGAAAGGATTTATTGATGATGCAAA; CTACTCCGTTGGCCTGTTGGATGAAGGAACAAACCTTGGAAATGTTATTGATAACTATGT; TTATGAACATACCCTGACAGGGAAAAATGCATTTTTTGTGGGAGATCTTGGAAAGATTGT; GAAGAAACACAGTCAATGGCAGAATGTAGTGGCTCAGATAAAGCCATTCTACACAGTGAA; GTGCAACTCTGCTCCAGCTGTACTTGAGATTTTGGCAGCTCTTGGAACCGGATTTGCTTG; TTCCAGTAAAAATGAAATGGCTTTAGTGCAAGAGTTGGGTGTACCTCCAGAAAACATTAT; TTACATAAGTCCTTGCAAGCAAGTGTCTCAGATAAAGTATGCAGCAAAAGTTGGAGTGAA; TATCCTGACATGTGACAATGAAATTGAATTGAAGAAAATTGCACGTAATCACCCAAATGC; CAAGGTCTTACTACATATTGCAACAGAAGATAATATTGGAGGTGAAGAGGGTAACATGAA; GTTTGGCACTACCCTGAAGAACTGTAGGCATCTCTTGGAATGTGCTAAGGAACTTGATGT; CCAAATAATTGGGGTTAAATTTCATGTTTCGAGTGCTTGCAAAGAATCTCAAGTATATGT; ACATGCTCTATCTGATGCTCGATGTGTGTTTGACATGGCTGGAGAAATTGGCTTTACGAT; GAACATGTTAGACATTGGTGGAGGATTCACGGGAACTGAATTTCAATTGGAAGAGGTTAA; TCATGTTATCAGCCCTCTGTTGGATATCTACTTTCCTGAAGGATCTGGTGTTAAGATAAT; TTCAGAACCCGGAAGCTACTATGTGTCTTCTGCATTTACACTCGCAGTTAATATCATAGC; AAAGAAAGTTGTTGAAAATGATAAATTTCCCTCTGGAGTAGAAAAAACCGGAAGTGATGA; ACCAGCCTTCATGTATTATATGAATGATGGTGTTTATGGTTCTTTTGCAAGTAAACTGTC; TGAGGACTTAAATACCATTCCAGAGGTTCACAAGAAATACAAGGAAGATGAGCCTCTGTT; TACAAGCAGCCTTTGGGGTCCATCCTGTGATGAGCTTGATCAAATTGTGGAAAGCTGTCT; TCTTCCTGAGCTGAATGTGGGAGATTGGCTTATCTTTGATAACATGGGAGCAGATTCTTT; CCATGAACCATCTGCTTTTAATGATTTTCAGAGGCCAGCCATTTATTACATGATGTCATT; CAGTGATTGGTATGAGATGCAAGATGCTGGAATTACTTCAGACTCAATGATGAAGAACTT; CTTCTTTGTGCCTTCTTGCATTCAGCTGAGCCAAGAAGACAGCTTTTCCGCTGAAGCTTA; AACAGGCATTAACGCTTCTTTAGATCTGAAGTTGCAGGTTAAGCTTGTCTGGTCAACATT; CCAGTGTGGAAAAATAATTTAAACAATCTTATTCTCTTAATTCTTTTGGCAACAAAAACT; ATTAGTAATAGCTATTTGGGACCAGACAAAATCAGCTTTCATCTATAATTCATTGGGGAT; AATGGGAGATTTAGATAATGTATCCAGATTTAAACCTACCAGTTTGTCCTACCCCTTAAG; CGTTTAAAATAAAATATGCAACAAAATGGATGACTTAGTGGAGATGGAAGCCCATTAATT; GGGTTCCCCATTAAATCGTTTACATACAAGAACACAGTTTTTATACTAAGGATTTGTGTT; TAAAGTCTTGTAAAGTTCATGTCTTTCACCCAGATATATCAAATGTTAGAAGACCAGTGT; GACTTCATTAGATAACGTTTAGTGTATTTAGAATGTGTAAATTTGTGCTTTGAACTGTAG; TTTAATAAATGTAAAATTGCATCATAGTATTTGTTGACCTAATGTAACCCTTGTATGATT; GCAATAAAATTTTGTGTAGATTTTAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_148174
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_148174.2, NP_680479.1
• RefSeq Size: 4202 bp
• RefSeq ORF: 1347 bp
• Locus ID: 51582
• UniProt ID: O14977
• Cytogenetics: 8q22.3
• Domains: Orn_Arg_deC_N
• Protein Families: Druggable Genome
• Summary: The protein encoded by this gene belongs to the antizyme inhibitor family, which plays a role in cell growth and proliferation by maintaining polyamine homeostasis within the cell. Antizyme inhibitors are homologs of ornithine decarboxylase (ODC, the key enzyme in polyamine biosynthesis) that have lost the ability to decarboxylase ornithine; however, retain the ability to bind to antizymes. Antizymes negatively regulate intracellular polyamine levels by binding to ODC and targeting it for degradation, as well as by inhibiting polyamine uptake. Antizyme inhibitors function as positive regulators of polyamine levels by sequestering antizymes and neutralizing their effect. This gene encodes antizyme inhibitor 1, the first member of this gene family that is ubiquitously expressed, and is localized in the nucleus and cytoplasm. Overexpression of antizyme inhibitor 1 gene has been associated with increased proliferation, cellular transformation and tumorigenesis. Gene knockout studies showed that homozygous mutant mice lacking functional antizyme inhibitor 1 gene died at birth with abnormal liver morphology. RNA editing of this gene, predominantly in the liver tissue, has been linked to the progression of hepatocellular carcinoma. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2014]; Transcript Variant: This variant (2) lacks an internal 5' non-coding exon, thus has a shorter 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (1).