LYPLA2 (NM_007260) Human Untagged Clone

CAT#: SC324073

LYPLA2 (untagged)-Human lysophospholipase II (LYPLA2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LYPLA2
• Synonyms: APT-2; APT2; DJ886K2.4
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_007260.2 GTGTCTGCGGGAGAAAGAGGAGAATCGCCCAAGCGGCCTCGGAAGTCCCAGGGAGTGGAG
  GCCCCCGCCGTGGAGCCGTGTGGTGTATGTGTGGTAACACCATGTCTGTGCCCCTGCTCA
  CCGATGCTGCCACCGTGTCTGGAGCTGAGCGGGAAACGGCCGCGGTTATTTTTTTACATG
  GACTTGGAGACACAGGGCACAGCTGGGCTGACGCCCTCTCCACCATCCGGCTCCCTCACG
  TCAAGTACATCTGTCCCCATGCGCCTAGGATCCCTGTGACCCTCAACATGAAGATGGTGA
  TGCCCTCCTGGTTTGACCTGATGGGGCTGAGTCCAGATGCCCCAGAGGACGAGGCTGGCA
  TCAAGAAGGCAGCAGAGAACATCAAGGCCTTGATTGAGCATGAAATGAAGAACGGGATCC
  CTGCCAATCGAATCGTCCTGGGAGGCTTTTCACAGGGCGGGGCCCTGTCCCTCTACACGG
  CCCTCACCTGCCCCCACCCTCTGGCTGGCATCGTGGCGTTGAGCTGCTGGCTGCCTCTGC
  ACCGGGCCTTCCCCCAGGCAGCTAATGGCAGTGCCAAGGACCTGGCCATACTCCAGTGCC
  ATGGGGAGCTGGACCCCATGGTGCCCGTACGGTTTGGGGCCCTGACGGCTGAGAAGCTCC
  GGTCTGTTGTCACACCTGCCAGGGTCCAGTTCAAGACATACCCGGGTGTCATGCACAGCT
  CCTGTCCTCAGGAGATGGCAGCTGTGAAGGAATTTCTTGAGAAGCTGCTGCCTCCTGTCT
  AACTAGTCGCTGGCCCCAGTGCAGTACCCCAGCTCATGGGGGACTCAGCAAGCAAGCGTG
  GCACCATCTTGGATCTGAGCCGGTCGAGCCCCTGTCCCCACCCTTCCTGACCTGTCCTTT
  TCCCACAGGCCTCTGGGGGCAGGTGGCAAGGCCTGGCCGGGCCTTCCTTCCTGGCCTTAG
  CCACCTGGCTCTGTCTGCAGCAGGGGCAGGCTGCTTTCTTATCCATTTCCCTGGAGGCGG
  GCCCCCCTGGCAGCAGTATTGGAGGGGCTACAGGCAGCTGGAGAAAGGGGCCCAGCCGCT
  GACCCACTCACTCAGGACCTCACTCACTAGCCCCGCTTTGGGCCCCCTCCTGTGACCTCA
  GGGTTTGGCCCATGGGGCCCTCCCAGGCCCCTGCCCCAACTGATTCTGCCCAGATAATCG
  TGTCTCCTGCCTCCACTCAGCTGCTTCTCAGTCATGAATGTGGCCATGGCCCCGGGGTCC
  CCTTGCTGCTGTGGGCTCCCTGTCCCTGGGCAGGAGTGCTGGTGAGGAGGTGGAGCCTTT
  TGAGGGGGGCCTTCCCTCAGCTGTTTCCCCACACTGGGGGGCTGGGCCCTGCCTCCCCGT
  TACCCTCCTTCCCTGCAGGCCTGGAGCCTGTAGGGCTGGACTGAGGTTCAGGTCTCCCCC
  CAGCTGTCTCACCCCCACTTTGTCCCCACTCTAGAGCAGGGAGGCAGTGGGGGAGGAGTT
  GTGTCTCGTCTTCTGTCTCCATGTGGTTTTTGGGTGTTTTTCTTGTTGTGTCCTGGATTC
  CGATAAAATTAAAGAAATTGCTTCCTCAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_007260
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007260.2, NP_009191.1
• RefSeq Size: 1648 bp
• RefSeq ORF: 696 bp
• Locus ID: 11313
• UniProt ID: O95372
• Cytogenetics: 1p36.11
• Domains: abhydrolase_2
• Protein Pathways: Glycerophospholipid metabolism
• Gene Summary: Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. There are alternatively spliced transcript variants described for this gene but the full length nature is not known yet. [provided by RefSeq, Jul 2008]