MNDA (NM_002432) Human Untagged Clone

SKU SC323960

MNDA (untagged)-Human myeloid cell nuclear differentiation antigen (MNDA)

Product Data

• Type: Human Untagged Clone
• Target Symbol: MNDA
• Synonyms: PYHIN3
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002432.1 CTACAATCAAGATTGAGAGTGGCTCTAACAAGTGCCATTTTTCCTTGTTAGCTTTCATTT; CTCAGCCCTTTACAAGATTAAAATAGTCTGCAGTTTAATCTCTCCAAAGCTTTACGGACA; GTGATTCTGTCCTAAACAAGACAGTGACTCCAGGATTTCTGAAGACTATTGTGGAAGAAG; CATCCATTAAGGCCAAGCTATAACATCAGAAATGGTGAATGAATACAAGAAAATTCTTTT; GCTGAAAGGATTTGAGCTCATGGATGATTATCATTTTACATCAATTAAGTCCTTACTGGC; CTATGATTTAGGACTAACTACAAAAATGCAAGAGGAATACAACAGAATTAAGATTACAGA; TTTGATGGAAAAAAAGTTCCAAGGCGTTGCCTGTCTAGACAAACTAATAGAACTTGCCAA; AGATATGCCATCACTTAAAAACCTTGTTAACAATCTTCGAAAAGAGAAGTCAAAAGTTGC; TAAGAAAATTAAAACACAAGAAAAAGCTCCAGTGAAAAAAATAAACCAGGAAGAAGTGGG; TCTTGCGGCACCTGCACCCACCGCAAGAAACAAACTGACATCGGAAGCAAGAGGGAGGAT; TCCTGTAGCTCAGAAAAGAAAAACTCCAAACAAAGAAAAGACTGAAGCCAAAAGGAATAA; GGTGTCCCAAGAGCAGAGTAAGCCCCCAGGTCCCTCAGGAGCCAGCACATCTGCAGCTGT; GGATCATCCCCCACTACCCCAGACCTCATCATCAACTCCATCCAACACTTCGTTTACTCC; GAATCAGGAAACCCAGGCCCAACGGCAGGTGGATGCAAGAAGAAATGTTCCCCAAAACGA; CCCAGTGACAGTGGTGGTACTGAAAGCAACAGCGCCATTTAAATACGAGTCCCCAGAAAA; TGGGAAAAGCACAATGTTTCATGCTACAGTGGCCAGTAAGACTCAATATTTCCATGTGAA; AGTCTTCGACATCAACTTGAAAGAGAAATTTGTAAGGAAGAAGGTCATTACCATATCTGA; TTACTCTGAATGTAAAGGAGTAATGGAAATAAAGGAAGCATCATCTGTGTCTGACTTTAA; TCAAAATTTTGAGGTCCCAAACAGAATTATCGAAATAGCAAATAAAACTCCCAAGATCAG; TCAACTTTACAAGCAAGCATCTGGAACAATGGTGTATGGGTTGTTTATGTTACAAAAGAA; AAGCGTACACAAGAAGAACACAATTTATGAAATACAGGATAATACAGGATCCATGGATGT; AGTGGGGAGTGGAAAATGGCACAATATCAAGTGTGAGAAAGGAGATAAACTTCGACTCTT; CTGCCTTCAACTGAGAACAGTTGACCGCAAGCTGAAACTGGTGTGTGGAAGTCACAGCTT; CATCAAGGTCATCAAGGCCAAGAAAAACAAGGAAGGACCAATGAATGTTAATTGAAATAT; GAAAGCTGAAATGCAACAAACAACTTCCGCTTAAAACAATTAAGTTGTTAATAACTGTGA; TTTTGTAAATTTCAGTAATTCATTTAAATGATGTTTCAGTAGATATATTCTAGCATATTA; AGAGCTTTTATAACTGAGTTATAGATTAGTTTGCTTTCTGGAATAAAATTTTCTTCTTAT; ACTCTTCCTTTTTTTTAGATATTACATTTTGCTTTTATGACATTCACGAGGCAAAAAATA; AAATATCTTTTTTTGAAGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_002432
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002432.1, NP_002423.1
• RefSeq Size: 1670 bp
• RefSeq ORF: 1224 bp
• Locus ID: 4332
• UniProt ID: P41218
• Cytogenetics: 1q23.1
• Domains: HIN, PAAD_DAPIN
• Protein Families: Transcription Factors
• Summary: The myeloid cell nuclear differentiation antigen (MNDA) is detected only in nuclei of cells of the granulocyte-monocyte lineage. A 200-amino acid region of human MNDA is strikingly similar to a region in the proteins encoded by a family of interferon-inducible mouse genes, designated Ifi-201, Ifi-202, and Ifi-203, that are not regulated in a cell- or tissue-specific fashion. The 1.8-kb MNDA mRNA, which contains an interferon-stimulated response element in the 5-prime untranslated region, was significantly upregulated in human monocytes exposed to interferon alpha. MNDA is located within 2,200 kb of FCER1A, APCS, CRP, and SPTA1. In its pattern of expression and/or regulation, MNDA resembles IFI16, suggesting that these genes participate in blood cell-specific responses to interferons. [provided by RefSeq, Jul 2008]