PFKFB4 (NM_004567) Human Untagged Clone

SKU SC323929

PFKFB4 (untagged)-Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (PFKFB4)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PFKFB4
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004567.2 CTCATCCCGGCCCCGGGATGGCGTCCCCACGGGAATTGACACAGAACCCCCTGAAGAAGA; TCTGGATGCCATACAGCAATGGGCGGCCCGCTCTGCACGCTTGCCAGCGCGGTGTGTGCA; TGACCAACTGCCCAACTCTCATTGTCATGGTGGGCCTGCCCGCCAGGGGCAAGACCTACA; TCTCCAAGAAGCTGACTCGATACCTGAACTGGATTGGTGTGCCCACTCGGGAGTTCAATG; TTGGCCAGTATCGCCGGGACGTGGTCAAGACCTACAAATCTTTTGAATTTTTTCTCCCCG; ACAATGAAGAGGGCCTGAAAATCAGGAAGCAGTGTGCCCTGGCAGCCCTCCGTGACGTCC; GGCGGTTCCTTAGTGAGGAGGGGGGACATGTGGCGGTTTTTGATGCCACAAACACCACCC; GAGAACGGAGAGCGACCATCTTTAATTTTGGAGAACAGAATGGCTACAAGACCTTTTTTG; TCGAGTCCATCTGTGTGGATCCTGAGGTCATAGCTGCCAACATCGTGCAAGTGAAACTGG; GCAGCCCTGACTATGTCAACCGCGACAGTGATGAGGCTACGGAGGACTTCATGAGGCGCA; TTGAGTGCTATGAGAACTCCTACGAGTCGCTAGATGAGGACCTGGATAGGGACCTGTCCT; ATATCAAGATCATGGATGTGGGCCAGAGCTACGTGGTGAACCGTGTGGCTGACCACATCC; AGAGCCGCATCGTATATTACCTCATGAACATCCACGTGACCCCCCGCTCCATCTACCTCT; GCCGGCACGGGGAGAGCGAGCTCAACCTCAAGGGCCGGATTGGCGGGGACCCAGGACTGT; CCCCTCGGGGCAGGGAGTTTGCCAAGAGTCTAGCCCAGTTCATCAGTGACCAAAATATCA; AGGATCTGAAGGTCTGGACAAGCCAGATGAAGAGGACAATCCAGACGGCTGAGGCACTGG; GTGTGCCCTATGAACAGTGGAAGGTCCTCAACGAGATCGATGCGGGCGTCTGTGAGGAAA; TGACCTACGAGGAAATTCAGGATAATTATCCACTGGAGTTCGCCCTGCGGGACCAGGACA; AGTACCGGTACCGGTACCCTAAAGGGGAGTCCTACGAGGACCTGGTCCAGAGACTGGAGC; CTGTCATCATGGAGCTGGAGAGGCAAGAGAATGTGCTGGTCATCTGCCACCAGGCTGTGA; TGCGCTGCCTGCTGGCCTACTTCCTCGACAAGGCAGCAGAACAGCTGCCCTACCTCAAGT; GTCCGCTGCACACAGTCCTGAAGCTGACTCCTGTGGCATATGGTTGTAAAGTGGAGTCCA; TATTCCTGAACGTGGCTGCTGTGAACACGCACCGGGACAGGCCTCAGAACGTGGACATCT; CAAGACCTCCAGAGGAAGCCCTTGTCACGGTGCCTGCTCACCAGTGACCATGTTCATCCA; CTGTGACCACTAGGCAGGCACTGCTCTCTGCAGAGGGGGTCATTCCAGGCCCTCCAGTGT; GTGTGATAGTCACCATGCCATGCAGGGATATTCTTGAAGCCACACATGGCTGGCGGAACC; CAGAGCCCCCACCCCAGCCCACCTGGCTCTTTGTTGACAGTCGGCGACAAGGTTGTGCGT; GGCTCCTGACCTGCTGCTAAGAGTCACTTGACCAGACTGCATCTGCATGGGCTGCGCGGA; GGTTGCCCAGCCCCAGTTTCTTCCGGCGCAGCTCTTAGGTGTTCACTCTCGCCAGCTCAG; TTGGCTTTGTGAAGTGTGAAACCCTACAATGTGAAAGGAAAGTGCTTGCTGTGATGTTCC; TACTGTGGCCCAGCTGCCCAGCATGGACCTGGTGACTCTCCACAGGGCCTCTACCATCCT; CTCTGTGGCCACTTCCTGAGCCAGAGGCCAGGTCTTCATGGGGCCCTGAGCTTCTGCTGC; CTCTGGTGAGAGGGAGAGCCCTTCCCATCCTTACCCACCAGGAACTAGAGCCCCAACCAC; AGCAGATGCTTCCTGGGCAGCCACTGGCCAGGCCGTTGTATCCATGTCACCCTTAGTTGT; GGGCATTCATGAAAGCAATGCGCTTGCTTCAGCACATTGGGATGCACAGAAACGTGAGGG; CAGGGGGGCAGTTGTGGTCGCCCCTCCCCAGTTGTGCATGTACCCGGCTACTTAGGTATG; CTTAGGGGAGCTGGTGGCCAGGGGTGCCCCTTCAGGCCTGCTCTCACAGTGAAACCACTT; CAAGACAGGGCAGGAAGTTACCTTCCTGGTGCACACACCGAAGTGCTCGAGGGAGAGGCT; GCCGCCCCCTCTCCCCTTCCCTGGCTGTGGACACTGGCAGCCAATGGGGAGGCGGCCTCT; CCCCTGTTGCTGCCCGTGTCCACACAGGAATGTACACTGGTGGCAGAAACATGCATTTGC; CATAAATAATTCAGAAACACTTGTATGGTCCTGTAAAACTTCTCTGGTGTTTGTTGACTT; CTGCCTGTGGGTCTGAGGAAGGGAGAAGGCAGAACCCATAGGCAGTCCCTGGGCAAGGGA; GGATTTTCCCGGCCGCAGCACAGCCTTCCATTCATGTGCACGTGAGTCTGGTGAAAATGG; CCTTGTGAAGTCCAGTGTCTGTCATGCCCCTGAGTTCTTTTGGTCCCAGAGAGGCAGCCT; GGGCAGTCTGTTTACAGCTGGCAAACAGACTGGCTGGCAAGTTGTGGGGCTGGGTCAGAA; AGTGTCTGGACTTGTAATCGGGTGCCCTGCTGTCCACAGGTGGACTCTGGGGAAGCCAGG; CCCCTGGATCCCAAGTCCTTTGTGTCCGGAGCCTTTCCTGGCCTGTGCCTGGAGGGTAGC; ACATCTTTCCAGCCTCCCAGGGCTTTGCAGCCTGCAGATGCCACCTGTCCCTGAGAGAAG; TTAGCGTCCCTGGCCCTGGCCGGTGGTCTCTCATCCTTGTGTGCTGCTCTGCTAAGAGAT; GTCCAAGGCGGAGCCGGGGCAAGATCCTTCCAGACTCATCTGTCAGAGCCCCAAGCCCTT; TAGACCCAGAGCCCAAGGACCATGCCTTTGGGACATTAGGACTGCAGCCTTTGCTTCTGT; GTATTTTGGAGTTTTGGTGACTTTTGTCACCTGGACACACTCATTTGTTAGCCATAGTGG; GTTCCCTTGGTCAGCAACAGTGCATGTACCTCTGGATGTCATCTGAGGTGAGACCACCGA; GGCCTTTTCTCTCTGTGTACAGAGGGGAGTTAGGAGTTGCTGGACTGGATGCATTACGAG; GACTGGGGACAGGGTAGAGGGACATCCAGGGATCAGGGCATGAGTGGGGGCAACCCCCCG; GCCTCTGCCCTGGCATGGTCTCCGCATGGGCTGAGGTGTAGCTGATTGGCTGCCACATTT; CGGCCATGCTGGCTGGCGTGCCCATGTTGCAGATATTTTCCCGAGTTCCCCAGAATGGAT; GGTATTGAATCTCAGCCACATGCAACACTGTGTCCAGCATTCTTTGCAATAAATACTTTT; TAAAAAATAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_004567
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004567.2, NP_004558.1
• RefSeq Size: 3503 bp
• RefSeq ORF: 1410 bp
• Locus ID: 5210
• UniProt ID: Q16877
• Cytogenetics: 3p21.31
• Domains: 6PF2K, PGAM
• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism
• Summary: The protein encoded by this gene is one of four bifunctional kinase/phosphatases that regulate the concentration of the glycolytic byproduct fructose-2,6-bisphosphate (F2,6BP). The encoded protein is highly expressed in cancer cells and is induced by hypoxia. This protein is essential to the survival of cancer cells under conditions of hypoxia, because it increases the amount of F2,6BP and ATP at a time when the cell cannot produce much of them. This finding suggests that this protein may be a good target for disruption in cancer cells, hopefully imperiling their survival. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]; Transcript Variant: This variant (2) lacks an alternate coding exon in the 5' end compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus compared to isoform a.