ARG2 (NM_001172) Human Untagged Clone

CAT#: SC323830

ARG2 (untagged)-Human arginase, type II (ARG2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARG2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001172.3 CACGGGGCGGGCGGACGCTGGCGCGGGTAGGTAAGAGCAGCGGCGGGCGGTGGCGCTCAC
  TCCCGGCTTCCAACCGCGCGGAGCCTCTGCCTTGGAGATTCTCAGTGCTGCGGATCATGT
  CCCTAAGGGGCAGCCTCTCGCGTCTCCTCCAGACGCGAGTGCATTCCATCCTGAAGAAAT
  CCGTCCACTCCGTGGCTGTGATAGGAGCCCCGTTCTCACAAGGGCAGAAAAGAAAAGGAG
  TGGAGCATGGTCCCGCTGCCATAAGAGAAGCTGGCTTGATGAAAAGGCTCTCCAGTTTGG
  GCTGCCACCTAAAAGACTTTGGAGATTTGAGTTTTACTCCAGTCCCCAAAGATGATCTCT
  ACAACAACCTGATAGTGAATCCACGCTCAGTGGGTCTTGCCAACCAGGAACTGGCTGAGG
  TGGTTAGCAGAGCTGTGTCAGATGGCTACAGCTGTGTCACACTGGGAGGAGACCACAGCC
  TGGCAATCGGTACCATTAGTGGCCATGCCCGACACTGCCCAGACCTTTGTGTTGTCTGGG
  TTGATGCCCATGCTGACATCAACACACCCCTTACCACTTCATCAGGAAATCTCCATGGAC
  AGCCAGTTTCATTTCTCCTCAGAGAACTACAGGATAAGGTACCACAACTCCCAGGATTTT
  CCTGGATCAAACCTTGTATCTCTTCTGCAAGTATTGTGTATATTGGTCTGAGAGACGTGG
  ACCCTCCTGAACATTTTATTTTAAAGAACTATGATATCCAGTATTTTTCCATGAGAGATA
  TTGATCGACTTGGTATCCAGAAGGTCATGGAACGAACATTTGATCTGCTGATTGGCAAGA
  GACAAAGACCAATCCATTTGAGTTTTGATATTGATGCATTTGACCCTACACTGGCTCCAG
  CCACAGGAACTCCTGTTGTCGGGGGACTAACCTATCGAGAAGGCATGTATATTGCTGAGG
  AAATACACAATACAGGGTTGCTATCAGCACTGGATCTTGTTGAAGTCAATCCTCAGTTGG
  CCACCTCAGAGGAAGAGGCGAAGACTACAGCTAACCTGGCAGTAGATGTGATTGCTTCAA
  GCTTTGGTCAGACAAGAGAAGGAGGGCATATTGTCTATGACCAACTTCCTACTCCCAGTT
  CACCAGATGAATCAGAAAATCAAGCACGTGTGAGAATTTAGGAGACACTGTGCACTGACA
  TGTTTCACAACAGGCATTCCAGAATTATGAGGCATTGAGGGGATAGATGAATACTAAATG
  GTTGTCTGGGTCAATACTGCCTTAATGAGAACATTTACACATTCTCACAATTGTAAAGTT
  TCCCCTCTATTTTGGTGACCAATACTACTGTAAATGTATTTGGTTTTTTGCAGTTCACAG
  GGTATTAATATGCTACAGTACTATGTAAATTTAAAGAAGTCATAAACAGCATTTATTACC
  TTGGTATATCATACTGGTCTTGTTGCTGTTGTTCCTTCACATTTAAGTGGTTTTTCATCT
  TTCCTCCCTCCTCCCACAGCCTGGCTATACAGTGCATCCTTGAACTGTCAGCCCACAGCA
  GCAATATGCTTATTCTATCCACATCCCTAACATCATGCATTCACAAGGTCAAAGTTCTGG
  TCCACAAACCCTTCCCTATAGAAGTTCAATGGCTGCGAAAGAATTTGTAGTAAACCAGGC
  CTCCCAGGATGGCGAGCTCCAGTAAGATGATAATGGAAAGCAGCAGCTTGTTGGTTGTCA
  CTCTACAAAGAGAAGCAAAGTGGGGAGTAGTCAGAAGTTTGGATAACCTTCCTTCTAAAC
  ATTTTGGGGTTAGACCTGGGACCACGGCTGGATACTCTGAGGCTGTATGTTTGATCACAC
  AGCCACTTAGCAGGAAGTACTCATAAGGTTCTTTAGCTGTCACTTAGGGATAACACTGTC
  TACCTCACAGAAATGTTAAACTGAGACAATAAAAACCAAAGCATAAAATGGATTCTGAAA
  AAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_001172
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001172.3.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172.3, NP_001163.1
• RefSeq Size: 1981 bp
• RefSeq ORF: 1065 bp
• Locus ID: 384
• UniProt ID: P78540
• Cytogenetics: 14q24.1
• Domains: arginase
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• Gene Summary: Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exists (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type II isoform encoded by this gene, is located in the mitochondria and expressed in extra-hepatic tissues, especially kidney. The physiologic role of this isoform is poorly understood; it is thought to play a role in nitric oxide and polyamine metabolism. Transcript variants of the type II gene resulting from the use of alternative polyadenylation sites have been described. [provided by RefSeq, Jul 2008]