CAPZA1 (NM_006135) Human Untagged Clone

CAT#: SC323780

CAPZA1 (untagged)-Human capping protein (actin filament) muscle Z-line, alpha 1 (CAPZA1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CAPZA1
• Synonyms: CAPPA1; CAPZ; CAZ1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006135.2 GGCCAGAACAGCCCAAGATGGCCGACTTCGATGATCGTGTGTCGGATGAGGAGAAGGTAC
  GCATAGCTGCTAAATTCATCACTCATGCACCCCCAGGGGAATTTAATGAAGTATTCAATG
  ACGTTCGGCTACTACTTAATAATGACAATCTCCTCAGGGAAGGGGCAGCACATGCATTTG
  CCCAGTATAACATGGATCAGTTCACGCCTGTGAAGATAGAAGGATATGAAGATCAGGTCT
  TAATTACAGAGCACGGTGACCTGGGTAATAGCAGATTTTTAGATCCAAGAAACAAAATTT
  CCTTTAAATTTGACCACTTACGGAAAGAAGCAAGTGACCCCCAGCCAGAAGAAGCAGATG
  GAGGTCTGAAGTCTTGGAGAGAATCCTGTGACAGTGCTTTAAGAGCCTATGTGAAAGACC
  ATTATTCCAACGGCTTCTGTACTGTTTATGCTAAAACTATCGATGGGCAACAGACTATTA
  TTGCATGTATTGAAAGCCACCAGTTTCAGCCTAAAAACTTCTGGAATGGTCGTTGGAGAT
  CAGAGTGGAAGTTCACCATCACACCACCTACAGCCCAGGTGGTTGGCGTGCTTAAGATTC
  AGGTTCACTATTATGAAGATGGCAATGTTCAGTTGGTTAGTCATAAAGATGTACAGGATT
  CACTAACTGTTTCGAATGAAGCCCAAACTGCCAAGGAGTTTATTAAAATCATAGAGAATG
  CAGAAAATGAGTATCAGACAGCAATTAGTGAAAACTATCAAACAATGTCAGATACCACAT
  TCAAGGCCTTGCGCCGGCAGCTTCCAGTTACCCGCACCAAAATCGACTGGAACAAGATAC
  TCAGCTACAAGATTGGCAAAGAAATGCAGAATGCTTAAAGGCTGAATGTAGGATTCTTCA
  GTATGTGGAAAGACAAGGATTCAACGTGTGGTCATATGATAAATAAGTGATTTATAAACA
  AGAGTGATATTTTGCTAGGGCTTTCAAAGTTAACCGGTTTTCTAGCCTCATGGAATACTG
  TTGAACCTATAGCGTTGTCTTGATTCTTTTGTGTTCTCTGCCTTGTAATTTTCTGTTACT
  GCTATATCTACGTGTAAATCTTTTTTTCTTTTTTTTTTTTTTTTTTTGGTTAATTCTGCC
  ACATTTAATGTTGGTGAGAGAGTGATCTATCCTAATGACATTTTACTGTTTAAAAAAGTT
  TCCTAGCCATGAAGCCCTGCTACTGATTTAGACAAGGTATTATGGTCATTACTTTGTACC
  CCTATCCTTCCAAGCACTTCTGGTACTTCAGTCGTTTTTACTGATCCACCAACACCTAAA
  GAGGCTATGCTACAGTCTCTAGCTAAATGGAAGACACATTCATCCTTCTCCCTCTGACTG
  CTTTGATCATCATTTATTGCATCTCATAACTAATTTTCTAAAGTTTGGATTGGGACTTTT
  CAGGTCCTTTTTGGAGGGCAAAGGAAGTGCCAGCTTCTCTGGGGAACTTGTTTTTAAATC
  CAAAGACTTGAACCACATTCCCTGCACATGAACATGTTTGCTTTTATCCCTTCTCTCATT
  GTCTCCTTCCCATCTTAGTACCATTGTAGTTATAAACATCTGCATTTTTTAGAAGCATTT
  TACCCATTTATTTTTTTAAACATTCAAGAACTGCTGACGTACTGTGGATGTAGAGTATAA
  AACTTGAAAAATGCAGATGTTGAAGGAATAATAGGTATCTTGTGCTTTAATACTTTATGG
  CAGGATTGTACTATAAGCAAATGAATTAAACAGCTATGTAAATCATAAAGAAAAACTAAA
  AATGAACCAAAGTGAAAGGATAACTTCCAGGCAGTATCTTTCTATTGTAACCTGTTATTT
  AAGGAAATACTAGTGATTTCTTCTAAATAGGATGTAAAACTTCTTTCAAATTACTCTTCC
  TCAGTCCTGCCTGCCAAGAACTCAAGTGTAACTGTGATAAAATAACCTTTCCCAGGTATA
  TTGGCAGGTATGTGTGTAATCTCAGAATACACAGGTGACATAGATATGATATGACAACTG
  GTAATGGTGGATTCATTTACATTGTTTACACTTCTATGACCAGGCCTTAAGGGAAGGTCA
  GTTTTTTAAAAAACCAAGTAGTGTCTTCCTACCTATCTCCAGATACATGTCAAAAAAAGA
  AAGGTGTTTGTGCTTCCGTTTTGTTTCTGCTCAGTAATATAGTCAAGCAAGTTTGTTCCA
  GGTGACCCATTGAGCTGTGTATGCATTTTTGTTTATTTCAATAAAATATATTTGTATTAT
  TTGTCCTTCATACTATCCATCCATACCACACTATCTTCTGTATCAGGTAGTCTAATAGAA
  ATATACCTGTTTTGTTCTAAAATTGAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_006135
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006135.2, NP_006126.1
• RefSeq Size: 2758 bp
• RefSeq ORF: 861 bp
• Locus ID: 829
• UniProt ID: P52907
• Cytogenetics: 1p13.2
• Domains: F-actin_cap_A
• Gene Summary: CAPZA1 is a member of the F-actin capping protein alpha subunit family. This gene encodes the alpha subunit of the barbed-end actin binding protein. The protein regulates growth of the actin filament by capping the barbed end of growing actin filaments. [provided by RefSeq, Jul 2008]