SIAH2 (NM_005067) Human Untagged Clone

CAT#: SC323731

SIAH2 (untagged)-Human seven in absentia homolog 2 (Drosophila) (SIAH2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SIAH2
• Synonyms: hSiah2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005067.5 GGACGCGTGGGCGGACGCGTGGGCTGTTCCAGAAGCGGGTGGGCCAGGGCTCTGCGCACC
  GCTGGGGTTCGGGGCCCGGGACGCCGCCGGGAGGAGGGCACCGCGCGGGGTCCGACGCGG
  AGGCGTGCTCGGAACGCCGGGGGCTGCGGAGTGCATCAGCGCGGTCCAGCCCTCCGCCTG
  CCGGGCGCCGAGCGTCTCCGCCGCCCGGACCTGGGCTGGGCGCCGTGGCGTTGCCTCGGA
  GCTCGCTGCCCGCGGGGCGCGCACCGCCTTGACCCGGGCGGCCCCGCGGCAGGCAGGCGC
  CCGCAGTTCCATGGTTGGTTCGGAGCGCGATGAGCCGCCCGTCCTCCACCGGCCCCAGCG
  CTAATAAACCCTGCAGCAAGCAGCCGCCGCCGCAGCCCCAGCACACTCCGTCCCCGGCTG
  CGCCCCCGGCCGCCGCCACCATCTCGGCTGCGGGCCCCGGCTCGTCCGCGGTGCCCGCCG
  CGGCGGCGGTGATCTCGGGCCCCGGCGGCGGCGGCGGGGCCGGCCCGGTGTCCCCGCAGC
  ACCACGAGCTGACCTCGCTCTTCGAGTGTCCGGTCTGCTTTGACTATGTCCTGCCTCCTA
  TTCTGCAGTGCCAGGCCGGGCACCTGGTGTGTAACCAATGCCGCCAGAAGTTGAGCTGCT
  GCCCGACGTGCAGGGGCGCCCTGACGCCCAGCATCAGGAACCTGGCTATGGAGAAGGTGG
  CCTCGGCAGTCCTGTTTCCCTGTAAGTATGCCACCACGGGCTGTTCCCTGACCCTGCACC
  ATACGGAGAAACCAGAACATGAAGACATATGTGAATACCGTCCCTACTCCTGCCCATGTC
  CTGGTGCTTCCTGCAAGTGGCAGGGGTCCCTGGAAGCTGTGATGTCCCATCTCATGCACG
  CCCACAAGAGCATTACCACCCTTCAGGGAGAAGACATCGTCTTTCTAGCTACAGACATTA
  ACTTGCCAGGGGCTGTCGACTGGGTGATGATGCAGTCATGTTTTGGCCATCACTTCATGC
  TGGTGCTGGAGAAACAAGAGAAGTACGAAGGCCACCAGCAGTTTTTTGCCATCGTCCTGC
  TCATTGGCACCCGCAAGCAAGCCGAGAACTTTGCCTACAGACTGGAGTTGAATGGGAACC
  GGCGGAGATTGACCTGGGAGGCCACGCCCCGTTCGATTCATGACGGTGTGGCTGCGGCCA
  TCATGAACAGCGACTGCCTTGTTTTCGACACAGCCATAGCACATCTTTTTGCAGATAATG
  GGAACCTTGGAATCAATGTTACTATTTCTACATGTTGTCCATGATGTGACTTTCGTAAAC
  CTTCAAAATTATTTGGGCATAGTGCTCTATGTTTAATAAAGGTTTTTATAGATGTTTTAT
  TCCATATGTCTTCACAAGTCAGGACCCACAATTACCCGTGTTTTGTTTGAACAGCAGTGT
  CCCATCTGGCTTCGACCCAACAAAGTTCATTAACCTGGGATGAATGGGGTTGGCCTGTTG
  GTGATTTGGATGCTGTTCTGTGATCTAAAACAACTCTTATTGAATTGTATTTACTCCCTA
  AACAACACTTGACAGGCTGTTGCACAGGGCTTCTATAAGATCAGTGTGTTAGGAATGGGA
  GGCCCCTTCCTGCCTGCCTTCCCATATTGGTCCCTTGACATTGACAAAAGCACAGTGACT
  GTCAGCAGATTCCTTTACTTTTGTTTGTGGGAGGTAGGAATTGTTTTAATGCATTTTAAA
  CAGTGTTTCTGAAATTGGATGGCTGGCTAATAGACACTGAATCACCCGGAGTGCTTATCT
  TAAAATTGCAGATTTAGGGAGCCTGCCAATTTAACAGTCTCATCAGGTGATTCTTTTCAA
  CAGTAATGTTTGAGAATTACTGGGTTAAATTGTGGGAAAGGGTCCAGATTTTAAAGGTGC
  TTTAAGGTTGCCCTCTGCCGATACTGTTTGTCTTTCTACTGTTTCATCCCCTAACTTCCC
  CCAACCCTCAAATTAAAACTAGAACTATAGATCCACATGAACGCACGCCTGAGATTTGGC
  CACTCACCTATGTTTTGGGTGGATTGCCTAGGAAAGCAAGTCATATGGCCATTGATAGTT
  CTCATGTAATTAGTTTTGCTCACCACTAGTACAGATGACCCGTTTACACGTGGCTTCCCT
  CGGAAGCCCTCCTCAACAGTAGCTGGTGTGAAAGACTAAATCAGTAGAGTTGGAAAAGCT
  TTATAACCGGTGTGTCATATGCTTGCTATTTAAAGCTGTGTGTTGGTTTTGTTTTTCTGC
  CACATTCACTAGTTTTTTAATAAATATTTTCCAAAAATGAAAAAAAAAAAAAAAAAAAAA
  AAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_005067
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005067.5, NP_005058.3
• RefSeq Size: 2632 bp
• RefSeq ORF: 975 bp
• Locus ID: 6478
• UniProt ID: O43255
• Cytogenetics: 3q25.1
• Domains: Sina
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in regulating cellular response to hypoxia. [provided by RefSeq, Jul 2008]