AP1M1 (NM_032493) Human Untagged Clone

CAT#: SC323715

AP1M1 (untagged)-Human adaptor-related protein complex 1, mu 1 subunit (AP1M1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AP1M1
• Synonyms: AP47; CLAPM2; CLTNM; MU-1A; mu1A
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_032493.2 CCCACGCGTCCGGTGAGCTGTCGCGGGCGGCGCCCGGCCTTGCTCAACGCCCAGCAGTCC
  CCACCGTCGCTGCCGCCGCCACCGCCCTCGGCCGCTGCCGAGGCCTCCTGCAGCCATCAT
  GTCCGCCAGCGCCGTCTACGTGCTGGACCTGAAGGGCAAGGTGCTCATCTGCCGGAACTA
  CCGTGGCGACGTGGACATGTCAGAGGTGGAGCACTTCATGCCCATCCTGATGGAGAAGGA
  GGAGGAGGGGATGCTGTCGCCCATCCTGGCCCACGGGGGGGTCCGTTTCATGTGGATCAA
  ACACAACAACCTGTATCTGGTTGCCACATCCAAGAAGAACGCGTGCGTGTCGCTGGTCTT
  TTCTTTCCTCTATAAGGTGGTGCAGGTGTTTTCCGAGTACTTCAAGGAGCTGGAGGAGGA
  GAGCATCCGGGACAACTTTGTTATCATCTACGAGCTGCTGGACGAGCTCATGGACTTCGG
  CTACCCCCAGACCACCGACAGCAAGATCCTGCAGGAGTACATCACTCAGGAAGGCCACAA
  GCTGGAAACAGGGGCCCCGCGGCCACCAGCCACCGTCACCAACGCGGTGTCCTGGCGGTC
  CGAAGGCATCAAGTATCGGAAGAATGAGGTGTTCTTGGACGTCATCGAGTCTGTCAACCT
  CTTGGTCAGCGCCAACGGCAATGTCCTGCGCAGCGAGATCGTGGGCTCCATCAAGATGCG
  AGTCTTCCTCTCGGGCATGCCCGAGCTGCGCCTGGGCCTCAACGACAAGGTCCTCTTTGA
  CAACACGGGCCGCGGCAAAAGCAAATCCGTGGAGCTGGAGGATGTGAAGTTCCACCAGTG
  TGTGCGGCTATCACGCTTCGAGAATGACCGCACCATCTCCTTCATCCCACCCGACGGCGA
  GTTCGAGCTCATGTCCTACCGTCTCAACACCCACGTCAAGCCTTTGATATGGATCGAGTC
  GGTGATCGAGAAGCACTCCCACAGCCGCATCGAGTACATGATCAAGGCCAAAAGCCAGTT
  CAAGCGGCGGTCAACAGCCAACAACGTGGAGATCCACATTCCCGTGCCCAATGATGCCGA
  CTCACCCAAGTTCAAGACGACGGTGGGGAGCGTTAAGTGGGTCCCCGAGAACAGCGAGAT
  TGTGTGGTCCATCAAGTCCTTCCCGGGCGGCAAGGAGTACCTGATGCGGGCCCACTTCGG
  CCTGCCTAGTGTGGAGGCCGAAGACAAGGAGGGCAAGCCCCCGATCAGTGTCAAGTTCGA
  GATCCCTTACTTCACTACCTCCGGCATCCAGGTGCGCTACCTGAAGATCATTGAGAAGAG
  TGGGTACCAGGCCCTGCCCTGGGTGCGTTATATCACGCAGAATGGAGATTACCAGCTCCG
  GACCCAGTGAGGGGCTGTCGCAGCCAACACCCCGGCCTCGGGGCTCCTGGTGGCAGCACC
  AGGGGACACACCTGCCAAACCCACCAGATGGAGGGGCCCTCCCTGGTCTCTGGCCACCCT
  CCCAGCCTCTGCCCAGGGACCCCTGCCTTCCCCAGGCCATCTGCTCTGCCGTCGACACTC
  GTCTCAGAAGCCCCTTTCCCAGAAGAGGCTGGTCTTCAAGAAGTCTCGTTTCTTTGCCCC
  TGAAGTCAGTTTCAGGGGAAGGATGTGAAATTTTTCCGTGTAGAGGTTACAGCCTTTTAT
  GCTGTTGAGCTCCCAGGTACCAAAAAGCTTGGCCAACGCTTGCCAGCCAGCCAGCTGCAG
  GTGGCATCTGCCACGAAGGAAGCGCCAGCCTCGCCAGGCCAGCAGGGGCGTCGTTTTGTT
  GCCATTTTGTTGAACGTTATGGGTTTATGGGTGTTCCTGGAACTTGTCTTTGTGCATTCG
  TTGCTGTTTGTGTTACCCTCACTGTCCCCATGTCCCACCCACGTCCTACGGCACTCAGGA
  AGCACTTGGTGAGGACGAGCCCTCACCCTTCTTGTCTTCCTTCCCAGCAGCGCCCGCAGC
  GGGCCATTTACACGTCGAGGCTGGCACCTGGCGCGCTCGGGGGCCACTGTAGCGTCTGCC
  TGCTCCCTGGACTCGCAGGCCTCGCCTGTGGCGCCTTCCCAGGGCCAGCCTGGGTCACGA
  GATGCTGTCACTCAGCCAGATCAGTATTGACCCACCAGGGGAGGTGGGGTTTGGTGAGAG
  ACGCCAGCCTCAGACTTTTTCCCACTGAGGGTCCAGAGAGCGGGGCCACGTGTCACCCAC
  GTCTGCGCTTGGTCACCCGTCCTCCCCACCCTGTGTGTGTTTATGTCATAGTTACATTAA
  ATTCCATTCATTGAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_032493
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032493.2, NP_115882.1
• RefSeq Size: 2297 bp
• RefSeq ORF: 1272 bp
• Locus ID: 8907
• UniProt ID: Q9BXS5
• Cytogenetics: 19p13.11
• Domains: Adap_comp_sub
• Protein Pathways: Lysosome
• Gene Summary: The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks a coding exon in the middle region, as compared to variant 1. The reading frame is not affected and the resulting isoform (2) lacks an internal segment, as compared to isoform 1.