ROBO2 (NM_001128929) Human Untagged Clone

SKU SC323277

ROBO2 (untagged)-Human roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: ROBO2
• Synonyms: SAX3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC323277 representing NM_001128929.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGCCAGAAGACATGAACGTGTCACTAGAAGGATGTGGACATGGGCTCCGGGACTGTTGATGATGACT; GTGGTGTTTTGGGGTCATCAGGGGAATGGACAAGGCCAAGGATCGCGTCTTCGCCAGGAGGACTTTCCC; CCGCGGATTGTGGAGCATCCTTCCGATGTCATCGTCTCTAAGGGCGAGCCCACGACTCTGAACTGCAAG; GCGGAGGGCCGGCCAACGCCCACCATTGAGTGGTACAAAGATGGGGAGCGAGTGGAGACTGACAAGGAC; GATCCCCGGTCCCACAGGATGCTTCTGCCCAGCGGATCCTTATTCTTCTTGCGCATCGTGCACGGGCGC; AGGAGTAAACCTGATGAAGGAAGCTACGTTTGTGTTGCGAGGAACTATCTTGGTGAAGCAGTGAGTCGA; AATGCGTCTCTGGAAGTGGCATTGTTACGAGATGACTTCCGACAAAACCCCACAGATGTTGTAGTGGCA; GCTGGAGAGCCTGCAATCCTGGAGTGCCAGCCTCCCCGGGGACACCCAGAACCCACCATCTACTGGAAA; AAAGACAAAGTTCGAATTGATGACAAGGAAGAAAGAATAAGTATCCGTGGTGGAAAACTGATGATCTCC; AATACCAGGAAAAGTGATGCAGGGATGTATACTTGTGTTGGTACCAATATGGTGGGAGAAAGGGACAGT; GACCCAGCAGAGCTGACTGTCTTTGAACGACCCACATTTCTCAGGAGGCCAATTAACCAGGTGGTACTG; GAGGAAGAAGCTGTAGAATTTCGTTGTCAAGTCCAAGGAGATCCTCAACCAACTGTGAGGTGGAAAAAG; GATGATGCAGACTTGCCAAGAGGAAGGTATGACATCAAAGACGATTACACACTAAGAATTAAAAAGACC; ATGAGTACAGATGAAGGCACCTATATGTGTATTGCTGAGAATCGGGTTGGAAAAATGGAAGCCTCTGCT; ACACTCACCGTCCGAGCTCCCCCACAGTTTGTGGTTCGGCCAAGAGATCAGATTGTTGCTCAAGGTCGA; ACAGTGACATTTCCCTGTGAAACTAAAGGAAACCCACAGCCAGCTGTTTTTTGGCAGAAAGAAGGCAGC; CAGAACCTACTTTTCCCAAACCAACCCCAGCAGCCCAACAGTAGATGCTCAGTGTCACCAACTGGAGAC; CTCACAATCACCAACATTCAACGTTCCGACGCGGGTTACTACATCTGCCAGGCTTTAACTGTGGCAGGA; AGCATTTTAGCAAAAGCTCAACTGGAGGTTACTGATGTTTTGACAGATAGACCTCCACCTATAATTCTA; CAAGGCCCAGCCAACCAAACGCTGGCAGTGGATGGTACAGCGTTACTGAAATGTAAAGCCACTGGTGAT; CCTCTTCCTGTAATTAGCTGGTTAAAGGAGGGATTTACTTTTCCGGGTAGAGATCCAAGAGCAACAATT; CAAGAGCAAGGCACACTGCAGATTAAGAATTTACGGATTTCTGATACTGGCACTTATACTTGTGTGGCT; ACAAGTTCAAGTGGAGAGACTTCCTGGAGTGCAGTGCTGGATGTGACAGAGTCTGGAGCAACAATCAGT; AAAAACTATGATTTAAGTGACCTGCCAGGGCCACCATCCAAACCGCAGGTCACTGATGTTACTAAGAAC; AGTGTCACCTTGTCCTGGCAGCCAGGTACCCCTGGAACCCTTCCAGCAAGTGCATATATCATTGAGGCT; TTCAGCCAATCAGTGAGCAACAGCTGGCAGACCGTGGCAAACCATGTAAAGACCACCCTCTATACTGTA; AGAGGACTGCGGCCCAATACAATCTACTTATTCATGGTCAGAGCGATCAACCCCCAAGGTCTCAGTGAC; CCAAGTCCCATGTCAGATCCTGTGCGCACACAAGATATCAGCCCACCAGCACAAGGAGTGGACCACAGG; CAAGTGCAGAAAGAGCTAGGAGATGTCCTTGTCCGTCTTCATAATCCAGTTGTGCTGACTCCCACCACG; GTTCAGGTCACATGGACGGTTGATCGCCAACCCCAGTTTATCCAAGGCTACCGAGTGATGTATCGTCAG; ACTTCAGGTCTGCAGGCGACATCTTCGTGGCAGAATTTAGATGCCAAAGTCCCGACTGAACGAAGTGCT; GTCTTAGTCAACCTGAAAAAGGGGGTGACTTATGAAATTAAAGTACGGCCATATTTTAATGAGTTCCAA; GGAATGGATAGTGAATCTAAAACGGTTCGTACTACTGAAGAAGCCCCAAGTGCCCCACCACAGTCTGTC; ACTGTACTGACAGTTGGAAGCTACAATAGCACAAGTATTAGTGTTTCCTGGGATCCTCCTCCTCCAGAT; CACCAGAATGGAATTATCCAAGAATACAAGATCTGGTGTCTAGGAAATGAAACGCGATTCCATATCAAC; AAAACTGTGGATGCAGCCATTCGGTCCGTAATAATTGGTGGATTATTCCCAGGTATTCAATACCGGGTA; GAGGTTGCAGCTAGTACCAGTGCAGGGGTTGGAGTAAAGAGTGAGCCACAGCCAATAATAATCGGGAGA; CGCAATGAAGTTGTCATTACTGAAAACAATAACAGCATAACTGAGCAAATCACTGATGTGGTGAAGCAA; CCAGCCTTTATAGCTGGTATTGGTGGTGCCTGCTGGGTAATTCTGATGGGTTTTAGCATATGGTTGTAT; TGGCGAAGAAAGAAGAGGAAGGGACTCAGTAATTATGCTGTTACGTTTCAAAGAGGAGATGGAGGACTA; ATGAGCAATGGAAGCCGTCCAGGTCTTCTCAATGCTGGTGATCCCAGCTATCCATGGCTTGCTGATTCT; TGGCCAGCCACGAGCTTGCCAGTAAATAATAGCAACAGTGGCCCAAATGAGATTGGAAATTTTGGCCGT; GGAGATGTGCTGCCACCAGTTCCAGGCCAAGGGGATAAAACAGCAACGATGCTCTCAGATGGAGCCATT; TATAGTAGCATTGACTTCACTACCAAAACCAGTTACAACAGTTCCAGCCAAATAACACAGGCTACCCCA; TATGCCACGACACAGATCTTGCATTCCAACAGCATACATGAATTGGCTGTCGATCTGCCTGATCCACAA; TGGAAAAGCTCAATTCAGCAAAAAACAGATCTGATGGGATTTGGTTATTCTCTACCTGATCAGAACAAA; GGTAACAATGGTGGGAAAGGTGGAAAAAAGAAGAAAAATAAAAACTCTTCTAAACCACAGAAAAACAAT; GGATCCACTTGGGCCAATGTCCCTCTACCTCCCCCCCCAGTCCAGCCCCTTCCTGGCACGGAGCTGGAA; CACTATGCAGTGGAACAACAAGAAAATGGCTATGACAGTGATAGCTGGTGCCCACCATTGCCAGTACAA; ACTTACTTACACCAAGGTCTGGAAGATGAACTGGAAGAAGATGATGATAGGGTCCCAACACCTCCTGTT; CGAGGCGTGGCTTCTTCTCCTGCTATCTCCTTTGGACAGCAGTCCACTGCAACTCTTACTCCATCCCCA; CGGGAAGAGATGCAACCCATGCTGCAGGCTCACCTGGATGAGTTGACAAGAGCCTATCAGTTTGATATA; GCAAAACAAACATGGCACATTCAAAGCAATAATCAACCTCCACAGCCTCCAGTTCCACCGTTAGGTTAT; GTGTCTGGAGCCTTGATTTCTGATTTGGAAACGGATGTTGCAGATGATGATGCCGACGACGAAGAGGAA; GCTTTAGAAATCCCCAGGCCCCTGAGAGCACTGGACCAGACTCCTGGATCCAGCATGGACAATCTAGAC; AGCTCTGTGACAGGAAAAGCCTTTACCTCCTCTCAAAGACCTCGACCTACCAGCCCATTTTCTACTGAC; AGTAACACCAGTGCAGCCCTGAGTCAAAGTCAGAGGCCTCGGCCCACTAAAAAACACAAGGGAGGGCGG; ATGGACCAACAACCAGCATTGCCTCATCGAAGGGAAGGAATGACAGATGAGGAGGCCTTGGTGCCCTAT; AGCAAGCCCAGTTTCCCATCTCCAGGTGGCCACAGCTCATCAGGAACAGCTTCTTCTAAGGGATCCACT; GGACCTAGGAAAACCGAGGTGTTGAGAGCAGGCCACCAGCGCAATGCCAGCGACCTTCTTGACATAGGA; TATATGGGCTCCAACAGTCAAGGACAGTTTACAGGTGAATTATAG; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001128929
• Insert Size: 4185 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001128929.3
• RefSeq Size: 8393 bp
• RefSeq ORF: 4185 bp
• Locus ID: 6092
• UniProt ID: Q9HCK4
• Cytogenetics: 3p12.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Axon guidance
• MW: 153.1 kDa
• Summary: The protein encoded by this gene belongs to the ROBO family, part of the immunoglobulin superfamily of proteins that are highly conserved from fly to human. The encoded protein is a transmembrane receptor for the slit homolog 2 protein and functions in axon guidance and cell migration. Mutations in this gene are associated with vesicoureteral reflux, characterized by the backward flow of urine from the bladder into the ureters or the kidney. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]; Transcript Variant: This variant (1) represents the use of an alternate promoter and has multiple differences compared to variant 4. These differences result in a distinct 5' UTR and the use of an alternate start codon compared to variant 4. The encoded protein (isoform ROBO2a; PMID 16829019) has a distinct N-terminus and is shorter than isoform 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.