Metabotropic Glutamate Receptor 8 (GRM8) (NM_001127323) Human Untagged Clone

SKU SC323233

GRM8 (untagged)-Human glutamate receptor, metabotropic 8 (GRM8), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: Metabotropic Glutamate Receptor 8
• Synonyms: GLUR8; GPRC1H; mGlu8; MGLUR8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC323233 representing NM_001127323.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGTATGCGAGGGAAAGCGATCAGCCTCTTGCCCTTGTTTCTTCCTCTTGACCGCCAAGTTCTACTGG; ATCCTCACAATGATGCAAAGAACTCACAGCCAGGAGTATGCCCATTCCATACGGGTGGATGGGGACATT; ATTTTGGGGGGTCTCTTCCCTGTCCACGCAAAGGGAGAGAGAGGGGTGCCTTGTGGGGAGCTGAAGAAG; GAAAAGGGGATTCACAGACTGGAGGCCATGCTTTATGCAATTGACCAGATTAACAAGGACCCTGATCTC; CTTTCCAACATCACTCTGGGTGTCCGCATCCTCGACACGTGCTCTAGGGACACCTATGCTTTGGAGCAG; TCTCTAACATTCGTGCAGGCATTAATAGAGAAAGATGCTTCGGATGTGAAGTGTGCTAATGGAGATCCA; CCCATTTTCACCAAGCCCGACAAGATTTCTGGCGTCATAGGTGCTGCAGCAAGCTCCGTGTCCATCATG; GTTGCTAACATTTTAAGACTTTTTAAGATACCTCAAATCAGCTATGCATCCACAGCCCCAGAGCTAAGT; GATAACACCAGGTATGACTTTTTCTCTCGAGTGGTTCCGCCTGACTCCTACCAAGCCCAAGCCATGGTG; GACATCGTGACAGCACTGGGATGGAATTATGTTTCGACACTGGCTTCTGAGGGGAACTATGGTGAGAGC; GGTGTGGAGGCCTTCACCCAGATCTCGAGGGAGATTGGTGGTGTTTGCATTGCTCAGTCACAGAAAATC; CCACGTGAACCAAGACCTGGAGAATTTGAAAAAATTATCAAACGCCTGCTAGAAACACCTAATGCTCGA; GCAGTGATTATGTTTGCCAATGAGGATGACATCAGGAGGATATTGGAAGCAGCAAAAAAACTAAACCAA; AGTGGGCATTTTCTCTGGATTGGCTCAGATAGTTGGGGATCCAAAATAGCACCTGTCTATCAGCAAGAG; GAGATTGCAGAAGGGGCTGTGACAATTTTGCCCAAACGAGCATCAATTGATGGATTTGATCGATACTTT; AGAAGCCGAACTCTTGCCAATAATCGAAGAAATGTGTGGTTTGCAGAATTCTGGGAGGAGAATTTTGGC; TGCAAGTTAGGATCACATGGGAAAAGGAACAGTCATATAAAGAAATGCACAGGGCTGGAGCGAATTGCT; CGGGATTCATCTTATGAACAGGAAGGAAAGGTCCAATTTGTAATTGATGCTGTATATTCCATGGCTTAC; GCCCTGCACAATATGCACAAAGATCTCTGCCCTGGATACATTGGCCTTTGTCCACGAATGAGTACCATT; GATGGGAAAGAGCTACTTGGTTATATTCGGGCTGTAAATTTTAATGGCAGTGCTGGCACTCCTGTCACT; TTTAATGAAAACGGAGATGCTCCTGGACGTTATGATATCTTCCAGTATCAAATAACCAACAAAAGCACA; GAGTACAAAGTCATCGGCCACTGGACCAATCAGCTTCATCTAAAAGTGGAAGACATGCAGTGGGCTCAT; AGAGAACATACTCACCCGGCGTCTGTCTGCAGCCTGCCGTGTAAGCCAGGGGAGAGGAAGAAAACGGTG; AAAGGGGTCCCTTGCTGCTGGCACTGTGAACGCTGTGAAGGTTACAACTACCAGGTGGATGAGCTGTCC; TGTGAACTTTGCCCTCTGGATCAGAGACCCAACATGAACCGCACAGGCTGCCAGCTTATCCCCATCATC; AAATTGGAGTGGCATTCTCCCTGGGCTGTGGTGCCTGTGTTTGTTGCAATATTGGGAATCATCGCCACC; ACCTTTGTGATCGTGACCTTTGTCCGCTATAATGACACACCTATCGTGAGGGCTTCAGGACGCGAACTT; AGTTACGTGCTCCTAACGGGGATTTTTCTCTGTTATTCAATCACGTTTTTAATGATTGCAGCACCAGAT; ACAATCATATGCTCCTTCCGACGGGTCTTCCTAGGACTTGGCATGTGTTTCAGCTATGCAGCCCTTCTG; ACCAAAACAAACCGTATCCACCGAATATTTGAGCAGGGGAAGAAATCTGTCACAGCGCCCAAGTTCATT; AGTCCAGCATCTCAGCTGGTGATCACCTTCAGCCTCATCTCCGTCCAGCTCCTTGGAGTGTTTGTCTGG; TTTGTTGTGGATCCCCCCCACATCATCATTGACTATGGAGAGCAGCGGACACTAGATCCAGAGAAGGCC; AGGGGAGTGCTCAAGTGTGACATTTCTGATCTCTCACTCATTTGTTCACTTGGATACAGTATCCTCTTG; ATGGTCACTTGTACTGTTTATGCCATTAAAACGAGAGGTGTCCCAGAGACTTTCAATGAAGCCAAACCT; ATTGGATTTACCATGTATACCACCTGCATCATTTGGTTAGCTTTCATCCCCATCTTTTTTGGTACAGCC; CAGTCAGCAGAAAAGATGTACATCCAGACAACAACACTTACTGTCTCCATGAGTTTAAGTGCTTCAGTA; TCTCTGGGCATGCTCTATATGCCCAAGGTTTATATTATAATTTTTCATCCAGAACAGAATGTTCAAAAA; CGCAAGAGGAGCTTCAAGGCTGTGGTGACAGCTGCCACCATGCAAAGCAAACTGATCCAAAAAGGAAAT; GACAGACCAAATGGCGAGGTGAAAAGTGAACTCTGTGAGAGTCTTGAAACCAACAGTAAGTCATCTGTA; GAGTTTCCGATGGTCAAGAGCGGGAGCACTTCCTAA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001127323
• Insert Size: 2727 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001127323.1
• RefSeq Size: 3873 bp
• RefSeq ORF: 2727 bp
• Locus ID: 2918
• UniProt ID: O00222
• Cytogenetics: 7q31.33
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 101.6 kDa
• Summary: L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) uses an alternate donor splice site at the penultimate exon compared to transcript variant 1, resulting in an isoform (b) of the same length, but with 16 different amino acids at the C-terminus compared to isoform a. This variant was designated as HmGluR8b by Malherbe et al, 1999 (PMID:10216218).