CHST8 (NM_001127895) Human Untagged Clone

SKU SC323052

CHST8 (untagged)-Human carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8 (CHST8), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: CHST8
• Synonyms: GalNAc4ST; GALNAC4ST1; PSS3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC323052 representing NM_001127895.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGACCCTGCGACCTGGAACAATGCGGCTGGCCTGCATGTTCTCTTCCATCCTGCTGTTCGGAGCTGCA; GGCCTCCTCCTCTTCATCAGCCTGCAGGACCCTACGGAGCTCGCCCCCCAGCAGGTGCCAGGAATAAAG; TTCAACATCAGGCCAAGGCAGCCCCACCACGACCTCCCACCAGGCGGCTCCCAGGATGGTGACTTGAAG; GAACCCACAGAGAGGGTCACTCGGGACTTATCCAGTGGGGCCCCGAGGGGCCGCAACCTGCCAGCGCCT; GACCAGCCTCAACCCCCGCTGCAGAGGGGAACCCGTCTGCGGCTCCGCCAGCGCCGTCGCCGTCTGCTC; ATCAAGAAAATGCCAGCTGCGGCGACCATCCCGGCCAACAGCTCGGACGCGCCCTTCATCCGGCCGGGA; CCCGGGACGCTGGATGGCCGCTGGGTCAGCCTGCACCGGAGCCAGCAGGAGCGCAAGCGGGTGATGCAG; GAGGCCTGCGCCAAGTACCGGGCGAGCAGCAGCCGCCGGGCCGTCACGCCCCGCCACGTGTCCCGTATC; TTCGTGGAGGACCGCCACCGCGTGCTCTACTGCGAGGTGCCCAAGGCCGGCTGCTCCAATTGGAAGCGG; GTGCTCATGGTGCTGGCCGGCCTGGCCTCGTCCACTGCCGACATCCAGCACAACACCGTCCACTATGGC; AGCGCTCTCAAGCGCCTGGACACCTTCGACCGCCAGGGTATCTTGCACCGTCTCAGCACCTACACCAAG; ATGCTCTTTGTCCGCGAGCCCTTCGAGAGGCTGGTGTCCGCCTTCCGCGACAAGTTTGAGCACCCCAAC; AGCTACTATCACCCGGTCTTCGGCAAGGCCATCCTGGCCCGGTACCGCGCCAATGCCTCTCGGGAGGCC; CTGCGGACCGGCTCTGGGGTGCGTTTTCCCGAGTTCGTCCAGTACCTGCTGGACGTGCACCGGCCCGTG; GGGATGGACATTCACTGGGACCATGTCAGCCGGCTCTGCAGCCCCTGCCTCATCGACTACGATTTCGTA; GGCAAGTTCGAGAGCATGGAGGACGATGCCAACTTCTTCCTGAGCCTCATCCGCGCGCCGCGGAACCTG; ACCTTCCCCCGGTTCAAGGACCGGCACTCGCAGGAGGCGCGGACCACAGCGAGGATCGCCCACCAGTAC; TTCGCCCAACTCTCGGCCCTGCAAAGGCAGCGCACCTACGACTTCTACTACATGGATTACCTGATGTTC; AACTATTCCAAGCCCTTTGCAGATCTGTACTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001127895
• Insert Size: 1275 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001127895.1
• RefSeq Size: 2340 bp
• RefSeq ORF: 1275 bp
• Locus ID: 64377
• UniProt ID: Q9H2A9
• Cytogenetics: 19q13.11
• Protein Families: Transmembrane
• MW: 48.8 kDa
• Summary: The protein encoded by this gene belongs to the sulfotransferase 2 family. It is predominantly expressed in the pituitary gland, and is localized to the golgi membrane. This protein catalyzes the transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. It is responsible for sulfation of GalNAc on luteinizing hormone (LH), which is required for production of the sex hormones. Mice lacking this enzyme, exhibit increased levels of circulating LH, and precocious sexual maturation of both male and female mice. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2011]; Transcript Variant: This variant (2) contains an additional 5' non-coding exon compared to variant 1. Variants 1-3 encode the same protein.