Perforin (PRF1) (NM_001083116) Human Untagged Clone

SKU SC322683

PRF1 (untagged)-Human perforin 1 (pore forming protein) (PRF1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: Perforin
• Synonyms: HPLH2; P1; PFP
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322683 GGCTGGTGCAAGGAGCCACAGTGGGCTGCCTGGGGGGCTGATGCCACCATTCCAGGAGCC; TCGGTGAAGAGAGGATATCCATCTGTGTAGCCGCTTCTCTATACGGGATTCCAGCTCCAT; GGCAGCCCGTCTGCTCCTCCTGGGCATCCTTCTCCTGCTGCTGCCCCTGCCCGTCCCTGC; CCCGTGCCACACAGCCGCACGCTCAGAGTGCAAGCGCAGCCACAAGTTCGTGCCTGGTGC; ATGGCTGGCCGGGGAGGGTGTGGACGTGACCAGCCTCCGCCGCTCGGGCTCCTTCCCAGT; GGACACACAAAGGTTCCTGCGGCCCGACGGCACCTGCACCCTCTGTGAAAATGCCCTACA; GGAGGGCACCCTCCAGCGCCTGCCTCTGGCGCTCACCAACTGGCGGGCCCAGGGCTCTGG; CTGCCAGCGCCATGTAACCAGGGCCAAAGTCAGCTCCACTGAAGCTGTGGCCCGGGATGC; GGCTCGTAGCATCCGCAACGACTGGAAGGTCGGGCTGGACGTGACTCCTAAGCCCACCAG; CAATGTGCATGTGTCTGTGGCCGGCTCACACTCACAGGCAGCCAACTTTGCAGCCCAGAA; GACCCACCAGGACCAGTACAGCTTCAGCACTGACACGGTGGAGTGCCGCTTCTACAGTTT; CCATGTGGTACACACTCCCCCGCTGCACCCTGACTTCAAGAGGGCCCTCGGGGACCTGCC; CCACCACTTCAACGCCTCCACCCAGCCCGCCTACCTCAGGCTTATCTCCAACTACGGCAC; CCACTTCATCCGGGCTGTGGAGCTGGGTGGCCGCATATCGGCCCTCACTGCCCTGCGCAC; CTGCGAGCTGGCCCTGGAAGGGCTCACGGACAACGAGGTGGAGGACTGCCTGACTGTCGA; GGCCCAGGTCAACATAGGCATCCACGGCAGCATCTCTGCCGAAGCCAAGGCCTGTGAGGA; GAAGAAGAAGAAGCACAAGATGACGGCCTCCTTCCACCAAACCTACCGGGAGCGCCATTC; GGAAGTGGTTGGCGGCCATCACACCTCCATTAACGACCTGCTGTTCGGGATCCAGGCCGG; GCCCGAGCAGTACTCAGCCTGGGTAAACTCGCTGCCCGGCAGCCCTGGCCTGGTGGACTA; CACCCTGGAACCCCTGCACGTGCTGCTGGACAGCCAGGACCCGCGGCGGGAGGCACTGAG; GAGGGCCCTGAGTCAGTACCTGACGGACAGGGCTCGCTGGAGGGACTGCAGCCGGCCGTG; CCCACCAGGGCGGCAGAAGAGCCCCCGAGACCCATGCCAGTGTGTGTGCCATGGCTCAGC; GGTCACCACCCAGGACTGCTGCCCTCGGCAGAGGGGCCTGGCCCAGCTGGAGGTGACCTT; CATCCAAGCATGGGGCCTGTGGGGGGACTGGTTCACTGCCACGGATGCCTATGTGAAGCT; CTTCTTTGGTGGCCAGGAGCTGAGGACGAGCACCGTGTGGGACAATAACAACCCCATCTG; GTCAGTGCGGCTGGATTTTGGGGATGTGCTCCTGGCCACAGGGGGGCCCCTGAGGTTGCA; GGTCTGGGATCAGGACTCTGGCAGGGACGATGACCTCCTTGGCACCTGTGATCAGGCTCC; CAAGTCTGGTTCCCATGAGGTGAGATGCAACCTGAATCATGGCCACCTAAAATTCCGCTA; TCATGCCAGGTGCTTGCCCCACCTGGGAGGAGGCACCTGCCTGGACTATGTCCCCCAAAT; GCTTCTGGGGGAGCCTCCAGGAAACCGGAGTGGGGCCGTGTGGTGAGAACAGTGAGCTTG; GAAAGGACCAGTATGCTTGGACTGAAGGGGTTCTCACAGTGGGAGCCAGGGCTGTCTTCG; TATTCCCATTAGACCAAGCTTATCCAACCCGAGGCCCGCATGCGGCCCAGGATGGCTTTG; AATGCGGCCCAACGCAAATTCGCAAACTTTCTTAAAACATTATGAGTTTCTTTTTGCTAT; TTTTTTTTTTTTTTTTAGCTCATCGGCTATCGTTAGTGCTAGTGGATTTTACATGTGGCC; CAACACAATTCTTCTTCCAACGTGGCCCAGAGAAGCCAAAAGATTGGATACGCATCAGAC; AGATGGAAAAGGGAGATTCAGACTGTTTTTCAGGGAGGTGGCTGGGTTTACACGCTAATC; CCGATTCACCCTGTCCAAACTGCCTAAGCCCTCCGCCATTCTCAAGCCCTGCAGTCACAG; CTACACAGATCACAGCTTCAGCCAGGAGCTGGGCAGAAGGCCAAGAGGCTGTTCCCACCA; GGCTGCTCAGGGCTGGTCTTTTAGGACCCTTCCCTTGAGCCCTCTATGGTGTGGCAAAGC; CTTCATTGCCTTAACTGGAGCCCCATCAGCTCCAGCTGCTCTGTCTTCTTTGCCCACAAT; GCTTTGCCCCTGAGACAAATGGAGGCCTGTCCTGACCTGTCTCACCATGTACATAGCTTG; ATAAAGGGCCAATAAATATGATGTTATGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001083116
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001083116.1, NP_001076585.1
• RefSeq Size: 2555 bp
• RefSeq ORF: 1668 bp
• Locus ID: 5551
• UniProt ID: P14222
• Cytogenetics: 10q22.1
• Protein Families: Druggable Genome
• Protein Pathways: Allograft rejection, Autoimmune thyroid disease, Graft-versus-host disease, Natural killer cell mediated cytotoxicity, Type I diabetes mellitus, Viral myocarditis
• Summary: This gene encodes a protein with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017]; Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.