DVL1 (NM_182779) Human Untagged Clone

CAT#: SC322649

DVL1 (untagged)-Human dishevelled, dsh homolog 1 (Drosophila) (DVL1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DVL1
• Synonyms: dishevelled, dsh homolog 1 (Drosophila); dishevelled 1; dishevelled 1 (homologous to Drosophila dsh); DVL; DVL, MGC54245; MGC54245; OTTHUMP00000003104
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322649 GCGGGTAGCGCCCCGCGTCCGAGCCTCGGGCCGGGCCCTGAGCGCGCGGGCTCCGCGCCG
  CCGCCGCGCCATGGCGGAGACCAAGATTATCTACCACATGGACGAGGAGGAGACGCCGTA
  CCTGGTCAAGCTGCCCGTGGCCCCCGAGCGCGTCACGCTGGCCGACTTCAAGAACGTGCT
  CAGCAACCGGCCCGTGCACGCCTACAAATTCTTCTTTAAGTCCATGGACCAGGACTTCGG
  GGTGGTGAAGGAGGAGATCTTTGATGACAATGCCAAGCTTCCCTGCTTCAACGGCCGCGT
  GGTCTCCTGGCTGGTCCTGGCTGAGGGTGCTCACTCGGATGCGGGGTCCCAGGGCACGGA
  CAGCCACACAGACCTGCCCCCGCCTCTTGAGCGGACAGGCGGCATCGGGGACTCCCGGCC
  CCCCTCCTTCCACCCGAATGTGGCCAGCAGCCGTGACGGGATGGACAACGAGACAGGCAC
  GGAGTCCATGGTCAGTCACCGGCGGGAGCGTGCCCGACGCCGGAACCGCGAGGAGGCCGC
  CCGGACCAATGGGCACCCAAGGGGAGACCGACGGCGGGATGTGGGGCTGCCCCCAGACAG
  CGCGTCCACCGCCCTCAGCAGCGAGCTTGAGTCCAGCAGCTTTGTGGACTCGGACGAGGA
  TGGCAGCACGAGCAGGCTCAGCAGCTCCACGGAGCAGAGCACCTCATCCAGACTCACCCG
  GAAGTACGCCAGCAGCTTGCTGAAGCACGGCTTCCTGCGGCACACGGTCAACAAGATCAC
  CTTCTCCGAGCAGTGCTACTACGTCTTCGGGGATCTCTGCAGCAATCTCGCCACCCTGAA
  CCTCAACAGTGGCTCCAGTGGGACTTCGGATCAGGACACGCTGGCCCCGCTGCCCCACCC
  GGCTGCCCCCTGGCCTCTGGGTCAGGGCTACCCCTACCAGTACCCGGGACCCCCACCCTG
  CTTCCCGCCTGCCTACCAGGACCCGGGCTTTAGCTATGGCAGCGGCAGCACCGGGAGTCA
  GCAGAGTGAAGGGAGCAAAAGCAGTGGGTCCACCCGGAGCAGCCGCCGGGCCCCGGGCCG
  TGAGAAGGAGCGTCGGGCGGCGGGAGCTGGGGGCAGTGGCAGTGAATCGGATCACACGGC
  ACCGAGTGGGGTGGGGAGCAGCTGGCGAGAGCGTCCGGCCGGCCAGCTCAGCCGTGGCAG
  CAGCCCACGCAGTCAGGCCTCGGCTACCGCCCCGGGGCTCCCCCCGCCCCACCCCACGAC
  CAAGGCCTATACAGTGGTGGGGGGGCCACCCGGGGGACCCCCTGTCCGGGAGCTGGCTGC
  CGTCCCCCCGGAATTGACAGGCAGCCGCCAGTCCTTCCAGAAGGCTATGGGGAACCCCTG
  CGAGTTCTTCGTGGACATCATGTGACTCGTGGCGCATGCCCCAGCCCTGCCTGAGGTGGG
  GAGCTGGCGGTCCTGCCGCATGCAGAGCTCGCGTGGGCTTGCCTTCGTGGGGGCCAGGAC
  GGGAGGCAGGGTGGGGGGCAGGCTGGACCACCACCATCTGCCCTGGCAGCCTGGCTGCTC
  CAGCTCCTGACAGCACCTGTGTCTGAGCAGCCGTGTTGGGGGCGCTCCCTCTCTGCCCCT
  CAGCGAGAGCCTCGGACCTCCCAACCCCTTGTGTCTGGTGGGGATCCCTCCTGGGATGAG
  GAAGACCCCCTCGGGCTCTCGGCTGACCCCCACCTCCTGCACAGCTGTGCCCAGGCCCCC
  AGGGTGGTCCACGCGGGGCAACCCCCTGCGGTGCACAGGCCCCCTGTCTGGAGTAGGGAT
  CTAATTTATTTATTTATTGCCTGGCCGGTGACTCGGGGGAGGAGGCGACCCTGTCATCTG
  TCCCACCTGCTGCTGCCCCTTGGAGCAGCCTGCACCTTCTCTCCTCCCATCCGGCAACAG
  TCTGAAAGTACGTGGAGGACGGGACCGGAAGACGAGAGAGGGCTGGACATCCTGCCCACC
  GTGTCCCAGCCAGGGCAGGGAGGGACCATGGCCCGCAGGGTCAAGGGGCCCCGATGTGCA
  CAGCTGCCACAGGGAGGGAGGTCTTGGGGAGATGGGCAGTCAGGTGGCCCGTCCTTGGTG
  AGTGCACACACTGCGCGCACACATCGCGGCCTTCCTGGCTTCTCTGGCCCCCACGTGTCT
  GTGCTGTAGATACTGTATCAAAGTCCCAGCGTTTAGATGGTTAACATAGAGCTGCTTCTG
  TGTAAATGCTGCTTATTTTAAACACTAAAAAGCGTTTAATTTTATGGGAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_182779
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182779.2, NP_877580.1
• RefSeq Size: 2286 bp
• RefSeq ORF: 1335 bp
• Locus ID: 1855
• Cytogenetics: 1p36.33
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Melanogenesis, Notch signaling pathway, Pathways in cancer, Wnt signaling pathway
• Gene Summary: DVL1, the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation, acting as a transducer molecule for developmental processes, including segmentation and neuroblast specification. DVL1 is a candidate gene for neuroblastomatous transformation. The Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1. The phenotypes of these diseases may be consistent with defects which might be expected from aberrant expression of a DVL gene during development. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3) lacks an internal nt segment including several exons in the CDS, as compared to variant 1. The resulting isoform (c) has identical N- and C-termini but lacks an internal aa segment, as compared to isoform a.