DVL1 (NM_182779) Human Tagged ORF Clone

SKU RG208406

DVL1 (tGFP-tagged) - Human dishevelled, dsh homolog 1 (Drosophila) (DVL1), transcript variant 3

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: DVL1
• Synonyms: dishevelled, dsh homolog 1 (Drosophila); dishevelled 1; dishevelled 1 (homologous to Drosophila dsh); DVL; DVL, MGC54245; MGC54245; OTTHUMP00000003104
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208406 representing NM_182779; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGAGACCAAGATTATCTACCACATGGACGAGGAGGAGACGCCGTACCTGGTCAAGCTGCCCGTGG; CCCCCGAGCGCGTCACGCTGGCCGACTTCAAGAACGTGCTCAGCAACCGGCCCGTGCACGCCTACAAATT; CTTCTTTAAGTCCATGGACCAGGACTTCGGGGTGGTGAAGGAGGAGATCTTTGATGACAATGCCAAGCTT; CCCTGCTTCAACGGCCGCGTGGTCTCCTGGCTGGTCCTGGCTGAGGGTGCTCACTCGGATGCGGGGTCCC; AGGGCACGGACAGCCACACAGACCTGCCCCCGCCTCTTGAGCGGACAGGCGGCATCGGGGACTCCCGGCC; CCCCTCCTTCCACCCGAATGTGGCCAGCAGCCGTGACGGGATGGACAACGAGACAGGCACGGAGTCCATG; GTCAGTCACCGGCGGGAGCGTGCCCGACGCCGGAACCGCGAGGAGGCCGCCCGGACCAATGGGCACCCAA; GGGGAGACCGACGGCGGGATGTGGGGCTGCCCCCAGACAGCGCGTCCACCGCCCTCAGCAGCGAGCTTGA; GTCCAGCAGCTTTGTGGACTCGGACGAGGATGGCAGCACGAGCAGGCTCAGCAGCTCCACGGAGCAGAGC; ACCTCATCCAGACTCACCCGGAAGTACGCCAGCAGCTTGCTGAAGCACGGCTTCCTGCGGCACACGGTCA; ACAAGATCACCTTCTCCGAGCAGTGCTACTACGTCTTCGGGGATCTCTGCAGCAATCTCGCCACCCTGAA; CCTCAACAGTGGCTCCAGTGGGACTTCGGATCAGGACACGCTGGCCCCGCTGCCCCACCCGGCTGCCCCC; TGGCCTCTGGGTCAGGGCTACCCCTACCAGTACCCGGGACCCCCACCCTGCTTCCCGCCTGCCTACCAGG; ACCCGGGCTTTAGCTATGGCAGCGGCAGCACCGGGAGTCAGCAGAGTGAAGGGAGCAAAAGCAGTGGGTC; CACCCGGAGCAGCCGCCGGGCCCCGGGCCGTGAGAAGGAGCGTCGGGCGGCGGGAGCTGGGGGCAGTGGC; AGTGAATCGGATCACACGGCACCGAGTGGGGTGGGGAGCAGCTGGCGAGAGCGTCCGGCCGGCCAGCTCA; GCCGTGGCAGCAGCCCACGCAGTCAGGCCTCGGCTACCGCCCCGGGGCTCCCCCCGCCCCACCCCACGAC; CAAGGCCTATACAGTGGTGGGGGGGCCACCCGGGGGACCCCCTGTCCGGGAGCTGGCTGCCGTCCCCCCG; GAATTGACAGGCAGCCGCCAGTCCTTCCAGAAGGCTATGGGGAACCCCTGCGAGTTCTTCGTGGACATCA; TG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208406 representing NM_182779; Red=Cloning site Green=Tags(s); ; MAETKIIYHMDEEETPYLVKLPVAPERVTLADFKNVLSNRPVHAYKFFFKSMDQDFGVVKEEIFDDNAKL; PCFNGRVVSWLVLAEGAHSDAGSQGTDSHTDLPPPLERTGGIGDSRPPSFHPNVASSRDGMDNETGTESM; VSHRRERARRRNREEAARTNGHPRGDRRRDVGLPPDSASTALSSELESSSFVDSDEDGSTSRLSSSTEQS; TSSRLTRKYASSLLKHGFLRHTVNKITFSEQCYYVFGDLCSNLATLNLNSGSSGTSDQDTLAPLPHPAAP; WPLGQGYPYQYPGPPPCFPPAYQDPGFSYGSGSTGSQQSEGSKSSGSTRSSRRAPGREKERRAAGAGGSG; SESDHTAPSGVGSSWRERPAGQLSRGSSPRSQASATAPGLPPPHPTTKAYTVVGGPPGGPPVRELAAVPP; ELTGSRQSFQKAMGNPCEFFVDIM; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182779
• ORF Size: 1332 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_182779.2, NP_877580.1
• RefSeq Size: 2286 bp
• RefSeq ORF: 1334 bp
• Locus ID: 1855
• Cytogenetics: 1p36.33
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Melanogenesis, Notch signaling pathway, Pathways in cancer, Wnt signaling pathway
• Summary: DVL1, the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation, acting as a transducer molecule for developmental processes, including segmentation and neuroblast specification. DVL1 is a candidate gene for neuroblastomatous transformation. The Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1. The phenotypes of these diseases may be consistent with defects which might be expected from aberrant expression of a DVL gene during development. [provided by RefSeq, Jul 2008]