TBX21 (NM_013351) Human Untagged Clone

CAT#: SC322597

TBX21 (untagged)-Human T-box 21 (TBX21)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBX21
• Synonyms: T-bet; T-PET; TBET; TBLYM
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_013351 edited
  GGACGCGTGGGCGAGGGCGTAGAAGCCAGGCGTCAGAGCCCGGGCTCCGGTGGGGTCCCC
  CACCCGGCCCTCGGGTCCCCCGCCCCCTGCTCCCTGCCCATCCCAGCCCACGCGACCCTC
  TCGCGCGCGGAGGGGCGGGTCCTCGACGGCTACGGGAAGGTGCCAGCCCGCCCCGGATGG
  GCATCGTGGAGCCGGGTTGCGGAGACATGCTGACGGGCACCGAGCCGATGCCGGGGAGCG
  ACGAGGGCCGGGCGCCTGGCGCCGACCCGCAGCACCGCTACTTCTACCCGGAGCCGGGCG
  CGCAGGACGCGGACGAGCGTCGCGGGGGCGGCAGCCTGGGGTCTCCCTACCCGGGGGGCG
  CCTTGGTGCCCGCCCCGCCGAGCCGCTTCCTTGGAGCCTACGCCTACCCGCCGCGACCCC
  AGGCGGCCGGCTTCCCCGGCGCGGGCGAGTCCTTCCCGCCGCCCGCGGACGCCGAGGGCT
  ACCAGCCGGGCGAGGGCTACGCCGCCCCGGACCCGCGCGCCGGGCTCTACCCGGGGCCGC
  GTGAGGACTACGCGCTACCCGCGGGACTGGAGGTGTCGGGGAAACTGAGGGTCGCGCTCA
  ACAACCACCTGTTGTGGTCCAAGTTTAATCAGCACCAGACAGAGATGATCATCACCAAGC
  AGGGACGGCGGATGTTCCCATTCCTGTCATTTACTGTGGCCGGGCTGGAGCCCACCAGCC
  ACTACAGGATGTTTGTGGACGTGGTCTTGGTGGACCAGCACCACTGGCGGTACCAGAGCG
  GCAAGTGGGTGCAGTGTGGAAAGGCCGAGGGCAGCATGCCAGGAAACCGCCTGTACGTCC
  ACCCGGACTCCCCCAACACAGGAGCGCACTGGATGCGCCAGGAAGTTTCATTTGGGAAAC
  TAAAGCTCACAAACAACAAGGGGGCGTCCAACAATGTGACCCAGATGATTGTGCTCCAGT
  CCCTCCATAAGTACCAGCCCCGGCTGCATATCGTTGAGGTGAACGACGGAGAGCCAGAGG
  CAGCCTGCAACGCTTCCAACACGCATATCTTTACTTTCCAAGAAACCCAGTTCATTGCCG
  TGACTGCCTACCAGAATGCCGAGATTACTCAGCTGAAAATTGATAATAACCCCTTTGCCA
  AAGGATTCCGGGAGAACTTTGAGTCCATGTACACATCTGTTGACACCAGCATCCCCTCCC
  CGCCTGGACCCAACTGTCAATTCCTTGGGGGAGATCACTACTCTCCTCTCCTACCCAACC
  AGTATCCTGTTCCCAGCCGCTTCTACCCCGACCTTCCTGGCCAGGCGAAGGATGTGGTTC
  CCCAGGCTTACTGGCTGGGGGCCCCCCGGGACCACAGCTATGAGGCTGAGTTTCGAGCAG
  TCAGCATGAAGCCTGCATTCTTGCCCTCTGCCCCTGGGCCCACCATGTCCTACTACCGAG
  GCCAGGAGGTCCTGGCACCTGGAGCTGGCTGGCCTGTGGCACCCCAGTACCCTCCCAAGA
  TGGGCCCGGCCAGCTGGTTCCGCCCTATGCGGACTCTGCCCATGGAACCCGGCCCTGGAG
  GCTCAGAGGGACGGGGACCAGAGGACCAGGGTCCCCCCTTGGTGTGGACTGAGATTGCCC
  CCATCCGGCCGGAATCCAGTGATTCAGGACTGGGCGAAGGAGACTCTAAGAGGAGGCGCG
  TGTCCCCCTATCCTTCCAGTGGTGACAGCTCCTCCCCTGCTGGGGCCCCTTCTCCTTTTG
  ATAAGGAAGCTGAAGGACAGTTTTATAACTATTTTCCCAACTGAGCAGATGACATGATGA
  AAGGAACAGAAACAGTGTTATTAGGTTGGAGGACACCGACTAATTTGGGAAACGGATGAA
  GGACTGAGAAGGCCCCCGCTCCCTCTGGCCCTTCTCTGTTTAGTAGTTGGTTGGGGAAGT
  GGGGCTCAAGAAGGATTTTGGGGTTCACCAGATGCTTCCTGGCCCACGATGAAACCTGAG
  AGGGGTGTCCCCTTGCCCCATCCTCTGCCCTAACTACAGTCGTTTACCTGGTGCTGCGTC
  TTGCTTTTGGTTTCCAGCTGGAGAAAAGAAGACAAGAAAGTCTTGGGCATGAAGGAGCTT
  TTTGCATCTAGTGGGTGGGAGGGGTCAGGTGTGGGACATGGGAGCAGGAGACTCCACTTT
  CTTCCTTTGTACAGTAACTTTCAACCTTTTCGTTGGCATGTGTGTTAATCCCTGATCCAA
  AAAGAACAAATACACGTATGTTATAACCATCAGCCCGCCAGGGTCAGGGAAAGGACTCAC
  CTGACTTTGGACAGCTGGCCTGGGCTCCCCCTGCTCAAACACAGTGGGGATCAGAGAAAA
  GGGGCTGGAAAGGGGGGAATGGCCCACATCTCAAGAAGCAAGATATTGTTTGTGGTGGTT
  GTGTGTGGGTGTGTGTTTTTTCTTTTTCTTTCTTTTTATTTTTTTTGAATGGGGGAGGCT
  ATTTATTGTACTGAGAGTGGTGTCTGGATATATTCCTTTTGTCTTCATCACTTTCTGAAA
  ATAAACATAAAACTGTTAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_013351
• Insert Size: 2600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013351.1, NP_037483.1
• RefSeq Size: 2589 bp
• RefSeq ORF: 1608 bp
• Locus ID: 30009
• UniProt ID: Q9UL17
• Cytogenetics: 17q21.32
• Domains: T-box
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells. [provided by RefSeq, Jul 2008]