OCLN (NM_002538) Human Untagged Clone

CAT#: SC322559

OCLN (untagged)-Human occludin (OCLN), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OCLN
• Synonyms: BLCPMG; PPP1R115; PTORCH1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322559 GTCCCCGGCTGAGCGCTGGCGGTCGGTGCGGCGTCAGGTGCGCCCGCCAGGTGAGCGCGC
  TCCCTGGCACCGTTGGCCCCCGGAGGGTCGGGCCCAGTTGCGGCGAGCGGATTGGTTTAT
  CTTGGAAGCTAAAGGGCATTGCTCATCCTGAAGATCAGCTGACCATTGACAATCAGCCAT
  GTCATCCAGGCCTCTTGAAAGTCCACCTCCTTACAGGCCTGATGAATTCAAACCGAATCA
  TTATGCACCAAGCAATGACATATATGGTGGAGAGATGCATGTTCGACCAATGCTCTCTCA
  GCCAGCCTACTCTTTTTACCCAGAAGATGAAATTCTTCACTTCTACAAATGGACCTCTCC
  TCCAGGAGTGATTCGGATCCTGTCTATGCTCATTATTGTGATGTGCATTGCCATCTTTGC
  CTGTGTGGCCTCCACGCTTGCCTGGGACAGAGGCTATGGAACTTCCCTTTTAGGAGGTAG
  TGTAGGCTACCCTTATGGAGGAAGTGGCTTTGGTAGCTACGGAAGTGGCTATGGCTATGG
  CTATGGTTATGGCTATGGCTACGGAGGCTATACAGACCCAAGAGCAGCAAAGGGCTTCAT
  GTTGGCCATGGCTGCCTTTTGTTTCATTGCCGCGTTGGTGATCTTTGTTACCAGTGTTAT
  AAGATCTGAAATGTCCAGAACAAGAAGATACTACTTAAGTGTGATAATAGTGAGTGCTAT
  CCTGGGCATCATGGTGTTTATTGCCACAATTGTCTATATAATGGGAGTGAACCCAACTGC
  TCAGTCTTCTGGATCTCTATATGGTTCACAAATATATGCCCTCTGCAACCAATTTTATAC
  ACCTGCAGCTACTGGACTCTACGTGGATCAGTATTCGTATCACTACTGTGTTGTGGATCC
  CCAGGAGGCCATTGCCATTGTACTGGGGTTCATGATTATTGTGGCTTTTGCTTTAATAAT
  TTTCTTTGCTGTGAAAACTCGAAGAAAGATGGACAGGTATGACAAGTCCAATATTTTGTG
  GGACAAGGAACACATTTATGATGAGCAGCCCCCCAATGTCGAGGAGTGGGTTAAAAATGT
  GTCTGCAGGCACACAGGACGTGCCTTCACCCCCATCTGACTATGTGGAAAGAGTTGACAG
  TCCCATGGCATACTCTTCCAATGGCAAAGTGAATGACAAGCGGTTTTATCCAGAGTCTTC
  CTATAAATCCACGCCGGTTCCTGAAGTGGTTCAGGAGCTTCCATTAACTTCGCCTGTGGA
  TGACTTCAGGCAGCCTCGTTACAGCAGCGGTGGTAACTTTGAGACACCTTCAAAAAGAGC
  ACCTGCAAAGGGAAGAGCAGGAAGGTCAAAGAGAACAGAGCAAGATCACTATGAGACAGA
  CTACACAACTGGCGGCGAGTCCTGTGATGAGCTGGAGGAGGACTGGATCAGGGAATATCC
  ACCTATCACTTCAGATCAACAAAGACAACTGTACAAGAGGAATTTTGACACTGGCCTACA
  GGAATACAAGAGCTTACAATCAGAACTTGATGAGATCAATAAAGAACTCTCCCGTTTGGA
  TAAAGAATTGGATGACTATAGAGAAGAAAGTGAAGAGTACATGGCTGCTGCTGATGAATA
  CAATAGACTGAAGCAAGTGAAGGGATCTGCAGATTACAAAAGTAAGAAGAATCATTGCAA
  GCAGTTAAAGAGCAAATTGTCACACATCAAGAAGATGGTTGGAGACTATGATAGACAGAA
  AACATAGAAGGCTGATGCCAAGTTGTTTGAGAAATTAAGTATCTGACATCTCTGCAATCT
  TCTCAGAAGGCAAATGACTTTGGACCATAACCCCGGAAGCCAAACCTCTGTGAGCATCAC
  AAAGTTTTGGTTGCTTTAACATCATCAGTATTGAAGCATTTTATAAATCGCTTTTGATAA
  TCAACTGGGCTGAACACTCCAATTAAGGATTTTATGCTTTAAACATTGGTTCTTGTATTA
  AGAATGAAATACTGTTTGAGGTTTTTAAGCCTTAAAGGAAGGTTCTGGTGTGAACTAAAC
  TTTCACACCCCAGACGATGTCTTCATACCTACATGTATTTGTTTGCATAGGTGATCTCAT
  TTAATCCTCTCAACCACCTTTCAGATAACTGTTATTTATAATCACTTTTTTCCACATAAG
  GAAACTGGGTTCCTGCAATGAAGTCTCTGAAGTGAAACTGCTTGTTTCCTAGCACACACT
  TTTGGTTAAGTCTGTTTTATGACTTCATTAATAATAAATTCCCTGGCCTTTCATATTTTA
  GCTACTATATATGTGATGATCTACCAGCCTCCCTATTTTTTTTCTGTTATATAAATGGTT
  AAAAGAGGTTTTTCTTAAATAATAAAGATCATGTAAAAGTAAAAAAAAAAAAAAAAAAAA
  AAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002538
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002538.2, NP_002529.1
• RefSeq Size: 2648 bp
• RefSeq ORF: 1569 bp
• Locus ID: 100506658
• UniProt ID: Q16625
• Cytogenetics: 5q13.2
• Domains: MARVEL
• Gene Summary: This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Both variants 1 and 2 encode isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.