DR5 (TNFRSF10B) (NM_003842) Human Untagged Clone

SKU SC322224

TNFRSF10B (untagged)-Human tumor necrosis factor receptor superfamily, member 10b (TNFRSF10B), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: DR5
• Synonyms: CD262; DR5; KILLER; KILLER/DR5; TRAIL-R2; TRAILR2; TRICK2; TRICK2A; TRICK2B; TRICKB; ZTNFR9
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003842 edited; GCCCCAAGTCAGCCTGGACACATAAATCAGCACGCGGCCGGAGAACCCCGCAATCTCTGC; GCCCACAAAATACACCGACGATGCCCGATCTACTTTAAGGGCTGAAACCCACGGGCCTGA; GAGACTATAAGAGCGTTCCCTACCGCCATGGAACAACGGGGACAGAACGCCCCGGCCGCT; TCGGGGGCCCGGAAAAGGCACGGCCCAGGACCCAGGGAGGCGCGGGGAGCCAGGCCTGGG; CTCCGGGTCCCCAAGACCCTTGTGCTCGTTGTCGCCGCGGTCCTGCTGTTGGTCTCAGCT; GAGTCTGCTCTGATCACCCAACAAGACCTAGCTCCCCAGCAGAGAGCGGCCCCACAACAA; AAGAGGTCCAGCCCCTCAGAGGGATTGTGTCCACCTGGACACCATATCTCAGAAGACGGT; AGAGATTGCATCTCCTGCAAATATGGACAGGACTATAGCACTCACTGGAATGACCTCCTT; TTCTGCTTGCGCTGCACCAGGTGTGATTCAGGTGAAGTGGAGCTAAGTCCCTGCACCACG; ACCAGAAACACAGTGTGTCAGTGCGAAGAAGGCACCTTCCGGGAAGAAGATTCTCCTGAG; ATGTGCCGGAAGTGCCGCACAGGGTGTCCCAGAGGGATGGTCAAGGTCGGTGATTGTACA; CCCTGGAGTGACATCGAATGTGTCCACAAAGAATCAGGTACAAAGCACAGTGGGGAAGCC; CCAGCTGTGGAGGAGACGGTGACCTCCAGCCCAGGGACTCCTGCCTCTCCCTGTTCTCTC; TCAGGCATCATCATAGGAGTCACAGTTGCAGCCGTAGTCTTGATTGTGGCTGTGTTTGTT; TGCAAGTCTTTACTGTGGAAGAAAGTCCTTCCTTACCTGAAAGGCATCTGCTCAGGTGGT; GGTGGGGACCCTGAGCGTGTGGACAGAAGCTCACAACGACCTGGGGCTGAGGACAATGTC; CTCAATGAGATCGTGAGTATCTTGCAGCCCACCCAGGTCCCTGAGCAGGAAATGGAAGTC; CAGGAGCCAGCAGAGCCAACAGGTGTCAACATGTTGTCCCCCGGGGAGTCAGAGCATCTG; CTGGAACCGGCAGAAGCTGAAAGGTCTCAGAGGAGGAGGCTGCTGGTTCCAGCAAATGAA; GGTGATCCCACTGAGACTCTGAGACAGTGCTTCGATGACTTTGCAGACTTGGTGCCCTTT; GACTCCTGGGAGCCGCTCATGAGGAAGTTGGGCCTCATGGACAATGAGATAAAGGTGGCT; AAAGCTGAGGCAGCGGGCCACAGGGACACCTTGTACACGATGCTGATAAAGTGGGTCAAC; AAAACCGGGCGAGATGCCTCTGTCCACACCCTGCTGGATGCCTTGGAGACGCTGGGAGAG; AGACTTGCCAAGCAGAAGATTGAGGACCACTTGTTGAGCTCTGGAAAGTTCATGTATCTA; GAAGGTAATGCAGACTCTGCCATGTCCTAAGTGTGATTCTCTTCAGGAAGTCAGACCTTC; CCTGGTTTACCTTTTTTCTGGAAAAAGCCCAACTGGACTCCAGTCAGTAGGAAAGTGCCA; CAATTGTCACATGACCGGTACTGGAAGAAACTCTCCCATCCAACATCACCCAGTGGATGG; AACATCCTGTAACTTTTCACTGCACTTGGCATTATTTTTATAAGCTGAATGTGATAATAA; GGACACTAAAAAAAAAAAAAAAAA;
• Restriction Sites: Please inquire
• ACCN: NM_003842
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_003842.3.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003842.3, NP_003833.3
• RefSeq Size: 4160 bp
• RefSeq ORF: 1323 bp
• Locus ID: 8795
• UniProt ID: O14763
• Cytogenetics: 8p21.3
• Domains: DEATH, TNFR
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Apoptosis, Cytokine-cytokine receptor interaction, Natural killer cell mediated cytotoxicity, p53 signaling pathway
• Summary: The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. Two transcript variants encoding different isoforms and one non-coding transcript have been found for this gene. [provided by RefSeq, Mar 2009]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.