Cytochrome p450 2J2 (CYP2J2) (NM_000775) Human Untagged Clone

CAT#: SC321730

CYP2J2 (untagged)-Human cytochrome P450, family 2, subfamily J, polypeptide 2 (CYP2J2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cytochrome p450 2J2
• Synonyms: CPJ2; CYPIIJ2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000775.2 CCACGCGTCCGCGCCGCCTGCTTGGACCGCAGAAGAGCAGGAGGACGTCTGAGCCATGCT
  CGCGGCGATGGGCTCTCTGGCGGCTGCCCTCTGGGCAGTGGTCCATCCTCGGACTCTCCT
  ACTGGGCACTGTCGCCTTTCTGCTCGCTGCTGACTTTCTCAAAAGACGGCGCCCAAAGAA
  CTACCCGCCGGGGCCCTGGCGCCTGCCCTTCCTTGGCAACTTCTTCCTTGTGGACTTCGA
  GCAGTCGCACCTGGAGGTTCAGCTGTTTGTGAAGAAATATGGGAACCTTTTTAGCTTGGA
  GCTTGGTGACATATCTGCAGTTCTTATTACTGGCTTGCCCTTAATCAAAGAAGCCCTTAT
  CCACATGGACCAAAACTTTGGGAACCGCCCCGTGACCCCTATGCGAGAACATATCTTTAA
  GAAAAATGGATTGATTATGTCAAGTGGCCAGGCATGGAAGGAGCAAAGAAGGTTCACTCT
  GACAGCACTAAGGAACTTTGGTTTAGGAAAGAAGAGCTTAGAGGAACGCATTCAGGAGGA
  GGCCCAACACCTCACTGAAGCAATAAAAGAGGAGAACGGACAGCCTTTTGACCCTCATTT
  CAAGATCAACAATGCAGTTTCCAATATCATTTGCTCCATCACCTTCGGAGAACGCTTTGA
  GTACCAGGATAGTTGGTTTCAGCAGCTGCTGAAGTTACTAGATGAAGTCACATACTTGGA
  GGCTTCAAAGACATGCCAGCTCTACAATGTCTTTCCATGGATAATGAAATTCCTGCCTGG
  ACCCCACCAAACTCTCTTCAGCAACTGGAAAAAACTGAAATTGTTTGTTTCTCATATGAT
  TGACAAACACAGAAAGGATTGGAATCCTGCAGAAACAAGAGACTTTATTGATGCTTACCT
  TAAAGAAATGTCAAAGCACACAGGCAATCCTACTTCAAGTTTCCATGAAGAAAACCTCAT
  CTGCAGCACCCTGGACCTCTTCTTTGCCGGAACCGAGACAACTTCCACAACTCTGCGATG
  GGCTCTGCTTTATATGGCCCTCTACCCAGAAATCCAAGAAAAAGTACAAGCTGAGATTGA
  CAGAGTGATTGGCCAGGGGCAGCAGCCGAGCACAGCCGCCCGGGAGTCCATGCCCTACAC
  CAATGCTGTCATCCATGAGGTGCAGAGAATGGGCAACATCATCCCCCTGAACGTTCCCAG
  GGAAGTGACAGTTGATACCACTTTGGCTGGGTACCACCTGCCCAAGGGTACCATGATCCT
  GACCAATTTGACGGCGCTGCACAGGGACCCCACAGAGTGGGCCACCCCTGACACATTCAA
  TCCGGACCATTTTCTGGAGAATGGACAGTTTAAGAAAAGGGAAGCCTTTATGCCTTTCTC
  AATAGGAAAGCGGGCATGCCTCGGAGAACAGTTGGCCAGGACTGAGCTGTTTATTTTCTT
  CACTTCCCTTATGCAAAAATTTACCTTCAGGCCCCCAAACAATGAGAAGCTGAGCCTGAA
  GTTTAGAATGGGTATCACCATTTCCCCAGTCAGTCACCGCCTCTGCGCTGTTCCTCAGGT
  GTAATATTGTTAAGAAAGAAAGGGGCAAGGAAAGTAAGAAGACATGGCACGTGTTCTGAA
  ACCACTGGTGTCTGCTCAGATGTGTTGGGACAAAATGAAAGTGACTTTCAAGAAAGATCA
  GAGGAATTTGACTCAGAGAAAACTAGATCCAAATCCCAGCTCTACTGTCTCGTCCGAATT
  AGCCTTGGGAAAATCATTTATATGCTAAATAATTTACCTTTTTATCTAGGAGATGAAAAG
  AGGATAATGTTTCCTTCCATAAAGAAAGTTCTTGTAAGAATCAAAAGAAATGGTGAGCTT
  TAAGTGGTTTGTAAACCATAAAACACATCATAAAAGTTCTATCTATAAAAAAAAAAAAAA
  AAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000775
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000775.2, NP_000766.2
• RefSeq Size: 1876 bp
• RefSeq ORF: 1509 bp
• Locus ID: 1573
• UniProt ID: P51589
• Cytogenetics: 1p32.1
• Domains: p450
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Arachidonic acid metabolism, Linoleic acid metabolism, Metabolic pathways
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is thought to be the predominant enzyme responsible for epoxidation of endogenous arachidonic acid in cardiac tissue. Multiple transcript variants have been found for this gene. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (1) is the protein-coding transcript.