Cytochrome p450 2J2 (CYP2J2) (NM_000775) Human Tagged ORF Clone

SKU RG207417

CYP2J2 (tGFP-tagged) - Human cytochrome P450, family 2, subfamily J, polypeptide 2 (CYP2J2)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Cytochrome p450 2J2
• Synonyms: CPJ2; CYPIIJ2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207417 representing NM_000775; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCTCGCGGCGATGGGCTCTCTGGCGGCTGCCCTCTGGGCAGTGGTCCATCCTCGGACTCTCCTACTGG; GCACTGTCGCCTTTCTGCTCGCTGCTGACTTTCTCAAAAGACGGCGCCCAAAGAACTACCCGCCGGGGCC; CTGGCGCCTGCCCTTCCTTGGCAACTTCTTCCTTGTGGACTTCGAGCAGTCGCACCTGGAGGTTCAGCTG; TTTGTGAAGAAATATGGGAACCTTTTTAGCTTGGAGCTTGGTGACATATCTGCAGTTCTTATTACTGGCT; TGCCCTTAATCAAAGAAGCCCTTATCCACATGGACCAAAACTTTGGGAACCGCCCCGTGACCCCTATGCG; AGAACATATCTTTAAGAAAAATGGATTGATTATGTCAAGTGGCCAGGCATGGAAGGAGCAAAGAAGGTTC; ACTCTGACAGCACTAAGGAACTTTGGTTTAGGAAAGAAGAGCTTAGAGGAACGCATTCAGGAGGAGGCCC; AACACCTCACTGAAGCAATAAAAGAGGAGAACGGACAGCCTTTTGACCCTCATTTCAAGATCAACAATGC; AGTTTCCAATATCATTTGCTCCATCACCTTCGGAGAACGCTTTGAGTACCAGGATAGTTGGTTTCAGCAG; CTGCTGAAGTTACTAGATGAAGTCACATACTTGGAGGCTTCAAAGACATGCCAGCTCTACAATGTCTTTC; CATGGATAATGAAATTCCTGCCTGGACCCCACCAAACTCTCTTCAGCAACTGGAAAAAACTGAAATTGTT; TGTTTCTCATATGATTGACAAACACAGAAAGGATTGGAATCCTGCAGAAACAAGAGACTTTATTGATGCT; TACCTTAAAGAAATGTCAAAGCACACAGGCAATCCTACTTCAAGTTTCCATGAAGAAAACCTCATCTGCA; GCACCCTGGACCTCTTCTTTGCCGGAACCGAGACAACTTCCACAACTCTGCGATGGGCTCTGCTTTATAT; GGCCCTCTACCCAGAAATCCAAGAAAAAGTACAAGCTGAGATTGACAGAGTGATTGGCCAGGGGCAGCAG; CCGAGCACAGCCGCCCGGGAGTCCATGCCCTACACCAATGCTGTCATCCATGAGGTGCAGAGAATGGGCA; ACATCATCCCCCTGAACGTTCCCAGGGAAGTGACAGTTGATACCACTTTGGCTGGGTACCACCTGCCCAA; GGGTACCATGATCCTGACCAATTTGACGGCGCTGCACAGGGACCCCACAGAGTGGGCCACCCCTGACACA; TTCAATCCGGACCATTTTCTGGAGAATGGACAGTTTAAGAAAAGGGAAGCCTTTATGCCTTTCTCAATAG; GAAAGCGGGCATGCCTCGGAGAACAGTTGGCCAGGACTGAGCTGTTTATTTTCTTCACTTCCCTTATGCA; AAAATTTACCTTCAGGCCCCCAAACAATGAGAAGCTGAGCCTGAAGTTTAGAATGGGTATCACCATTTCC; CCAGTCAGTCACCGCCTCTGCGCTGTTCCTCAGGTG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207417 representing NM_000775; Red=Cloning site Green=Tags(s); ; MLAAMGSLAAALWAVVHPRTLLLGTVAFLLAADFLKRRRPKNYPPGPWRLPFLGNFFLVDFEQSHLEVQL; FVKKYGNLFSLELGDISAVLITGLPLIKEALIHMDQNFGNRPVTPMREHIFKKNGLIMSSGQAWKEQRRF; TLTALRNFGLGKKSLEERIQEEAQHLTEAIKEENGQPFDPHFKINNAVSNIICSITFGERFEYQDSWFQQ; LLKLLDEVTYLEASKTCQLYNVFPWIMKFLPGPHQTLFSNWKKLKLFVSHMIDKHRKDWNPAETRDFIDA; YLKEMSKHTGNPTSSFHEENLICSTLDLFFAGTETTSTTLRWALLYMALYPEIQEKVQAEIDRVIGQGQQ; PSTAARESMPYTNAVIHEVQRMGNIIPLNVPREVTVDTTLAGYHLPKGTMILTNLTALHRDPTEWATPDT; FNPDHFLENGQFKKREAFMPFSIGKRACLGEQLARTELFIFFTSLMQKFTFRPPNNEKLSLKFRMGITIS; PVSHRLCAVPQV; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000775
• ORF Size: 1506 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000775.4
• RefSeq Size: 1876 bp
• RefSeq ORF: 1509 bp
• Locus ID: 1573
• UniProt ID: P51589
• Cytogenetics: 1p32.1
• Domains: p450
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Arachidonic acid metabolism, Linoleic acid metabolism, Metabolic pathways
• Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is thought to be the predominant enzyme responsible for epoxidation of endogenous arachidonic acid in cardiac tissue. Multiple transcript variants have been found for this gene. [provided by RefSeq, Jan 2016]