PDK1 (NM_002610) Human Untagged Clone

SKU SC321678

PDK1 (untagged)-Human pyruvate dehydrogenase kinase, isozyme 1 (PDK1), nuclear gene encoding mitochondrial protein

Product Data

• Type: Human Untagged Clone
• Target Symbol: PDK1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002610.3 GAAACCTGGCGTACTGGCTGTGGCTTCTCTAGCGGGACTCGGCATGAGGCTGGCGCGGCT; GCTTCGCGGAGCCGCCTTGGCCGGCCCGGGCCCGGGGCTGCGCGCCGCCGGCTTCAGCCG; CAGCTTCAGCTCGGACTCGGGCTCCAGCCCGGCGTCCGAGCGCGGCGTTCCGGGCCAGGT; GGACTTCTACGCGCGCTTCTCGCCGTCCCCGCTCTCCATGAAGCAGTTCCTGGACTTCGG; ATCAGTGAATGCTTGTGAAAAGACCTCATTTATGTTTCTGCGGCAAGAGTTGCCTGTCAG; ACTGGCAAATATAATGAAAGAAATAAGTCTCCTTCCAGATAATCTTCTCAGGACACCATC; CGTTCAATTGGTACAAAGCTGGTATATCCAGAGTCTTCAGGAGCTTCTTGATTTTAAGGA; CAAAAGTGCTGAGGATGCTAAAGCTATTTATGACTTTACAGATACTGTGATACGGATCAG; AAACCGACACAATGATGTCATTCCCACAATGGCCCAGGGTGTGATTGAATACAAGGAGAG; CTTTGGGGTGGATCCTGTCACCAGCCAGAATGTTCAGTACTTTTTGGATCGATTCTACAT; GAGTCGCATTTCAATTAGAATGTTACTCAATCAGCACTCTTTATTGTTTGGTGGAAAAGG; CAAAGGAAGTCCATCTCATCGAAAACACATTGGAAGCATAAATCCAAACTGCAATGTACT; TGAAGTTATTAAAGATGGCTATGAAAATGCTAGGCGTCTGTGTGATTTGTATTATATTAA; CTCTCCCGAACTAGAACTTGAAGAACTAAATGCAAAATCACCAGGACAGCCAATACAAGT; GGTTTATGTACCATCCCATCTCTATCACATGGTGTTTGAACTTTTCAAGAATGCAATGAG; AGCCACTATGGAACACCATGCCAACAGAGGTGTTTACCCCCCTATTCAAGTTCATGTCAC; GCTGGGTAATGAGGATTTGACTGTGAAGATGAGTGACCGAGGAGGTGGCGTTCCTTTGAG; GAAAATTGACAGACTTTTCAACTACATGTATTCAACTGCACCAAGACCTCGTGTTGAGAC; CTCCCGCGCAGTGCCTCTGGCTGGTTTTGGTTATGGATTGCCCATATCACGTCTTTACGC; ACAATACTTCCAAGGAGACCTGAAGCTGTATTCCCTAGAGGGTTACGGGACAGATGCAGT; TATCTACATTAAGGCTCTGTCAACAGACTCAATAGAAAGACTCCCAGTGTATAACAAAGC; TGCCTGGAAGCATTACAACACCAACCACGAGGCTGATGACTGGTGCGTCCCCAGCAGAGA; ACCCAAAGACATGACGACGTTCCGCAGTGCCTAGACACACTTGGGACATCGGAAAATCCA; AATGTGGCTTTTGTATTAAATTTGGAAGTGTGGCCCAGAGTTGCTCAGAATTGGAGCAGA; GCCTGAGACGTATCTGCAGATCCTGTCATCAGCTGGCAAGTCCAGGAGACTGTGTCATTT; AGAGACTGTGTTGTTAGTTATCCCTCAACATCTTCTAAGGTGGCAGGAAATAATATTGGA; AATAACATTTTAAAGTAAAAATTTTAAAGTTTAAAGAAGAGTTTTGCCACTTAAACAGGG; GAGCTTTGTCTGGAAAATACACTGAGTTGAAACACTTCATCCTTGGAAGGATTATATAAG; ATGAACAGTTGTGATAAATGTGTAGATTAGAGGGATGTGAATGGGCAGTTAGTCCAGTGC; CCTCATTTAAGAGGCCAAGATCCTGATTCAGAGGAGGCATCCTTTGCCCAGAGCTGCTTA; GCTAATCTGACCAAATGTTGGGAAAAATGTCTCACCTAACCCACTATTCCTTAATTATGG; ATTTTGTGAAAAACAATAGAACATGTTAATGAGTAATTTATATTAGTTCGATGTATTACA; ATTTTTTAGCTTTAAATTACAGTTTTCTTATAATGTTGAAATGTTTTAGAATCCTTTGAA; TCTAAGTATTTGTTTCCTAAATGAAACATTTGTACAACATTTGATGTTTTTACTTATGAA; ATATTCTCCTCCCCCAAGAAAATTTAAACTTTTTCTCTCTATTTAAAAGCTAAGAAATGT; TTTAAAGGAAAAATGAAATTATCTTCCTTTAGCTTATTTTTAAGGTAAAACAGCTTTTTA; CTCTGTTATTGTGGTAATGGACAGAATATTACATACAAAAATATTCTGGGAGAGCTTTTT; CCTAGTTGGTTTTAAATCATTGTGCCACCTGAAAGGTTTTTAGATTTTATAGGAGCTAAT; TTGTCCACCAGCATTAATGTAACACAGTGTAGTTATGAAAATATATTGAAGGACAGGAAG; TGGACACGAAGTGATTTTTGTAACCTGAGCAGTTAATGAATGTGCCAACATTTTCTAGGA; AGGGACAGCAAGAATATTCTGCTCTGTAGTTAAAATACTGGCTGGCTTTTGATGTCTTCA; TGCTTAATTGTGATCACTTTCTTGCACTGTGATGTTTTTACGTGAATATGTTGAAGTAGA; AGTCTACCATATTATTTTATAAAATGTTTTCTGTATGGCAATAAACTGAAAACATGGATC; AACCCTTCTTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002610
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002610.3, NP_002601.1
• RefSeq Size: 4576 bp
• RefSeq ORF: 1311 bp
• Locus ID: 5163
• UniProt ID: Q15118
• Cytogenetics: 2q31.1
• Domains: HATPase_c
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Fc epsilon RI signaling pathway, Neurotrophin signaling pathway, T cell receptor signaling pathway
• Summary: Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013]; Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.