KAP1 (TRIM28) (NM_005762) Human Untagged Clone

CAT#: SC321588

TRIM28 (untagged)-Human tripartite motif containing 28 (TRIM28)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KAP1
• Synonyms: KAP1; PPP1R157; RNF96; TF1B; TIF1B
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005762.2 GGCGCGCGGGCGAGCGGTTGTGCTTGTGCTTGTGGCGCGTGGTGCGGGTTTCGGCGGCGG
  CTGAGGAAGAAGCGCGGGCGGCGCCTTCGGGAGGCGAGCAGGCAGCAGTTGGCCGTGCCG
  TAGCAGCGTCCCGCGCGCGGCGGGCAGCGGCCCAGGAGGCGCGTGGCGGCGCTCGGCCTC
  GCGGCGGCGGCGGCGGCAGCGGCCCAGCAGTTGGCGGCGAGCGCGTCTGCGCCTGCGCGG
  CGGGCCCCGCGCCCCTCCTCCCCCCCTGGGCGCCCCCGGCGGCGTGTGAATGGCGGCCTC
  CGCGGCGGCAGCCTCGGCAGCAGCGGCCTCGGCCGCCTCTGGCAGCCCGGGCCCGGGCGA
  GGGCTCCGCTGGCGGCGAAAAGCGCTCCACCGCCCCTTCGGCCGCAGCCTCGGCCTCTGC
  CTCAGCCGCGGCGTCGTCGCCCGCGGGGGGCGGCGCCGAGGCGCTGGAGCTGCTGGAGCA
  CTGCGGCGTGTGCAGAGAGCGCCTGCGACCCGAGAGGGAGCCCCGCCTGCTGCCCTGTTT
  GCACTCGGCCTGTAGTGCCTGCTTAGGGCCCGCGGCCCCCGCCGCCGCCAACAGCTCGGG
  GGACGGCGGGGCGGCGGGCGACGGCACCGTGGTGGACTGTCCCGTGTGCAAGCAACAGTG
  CTTCTCCAAAGACATCGTGGAGAATTATTTCATGCGTGATAGTGGCAGCAAGGCTGCCAC
  CGACGCCCAGGATGCGAACCAGTGCTGCACTAGCTGTGAGGATAATGCCCCAGCCACCAG
  CTACTGTGTGGAGTGCTCGGAGCCTCTGTGTGAGACCTGTGTAGAGGCGCACCAGCGGGT
  GAAGTACACCAAGGACCATACTGTGCGCTCTACTGGGCCAGCCAAGTCTCGGGATGGTGA
  ACGTACTGTCTATTGCAACGTACACAAGCATGAACCCCTTGTGCTGTTTTGTGAGAGCTG
  TGATACTCTCACCTGCCGAGACTGCCAGCTCAATGCCCACAAGGACCACCAGTACCAGTT
  CTTAGAGGATGCAGTGAGGAACCAGCGCAAGCTCCTGGCCTCACTGGTGAAGCGCCTTGG
  GGACAAACATGCAACATTGCAGAAGAGCACCAAGGAGGTTCGCAGCTCAATCCGCCAGGT
  GTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATGGCCATCCTGCAGATCATGAA
  GGAGCTGAATAAGCGGGGCCGTGTGCTGGTCAATGATGCCCAGAAGGTGACTGAGGGGCA
  GCAGGAGCGCCTGGAGCGGCAGCACTGGACCATGACCAAGATCCAGAAGCACCAGGAGCA
  CATTCTGCGCTTTGCCTCTTGGGCTCTGGAGAGTGACAACAACACAGCCCTTTTGCTTTC
  TAAGAAGTTGATCTACTTCCAGCTGCACCGGGCCCTCAAGATGATTGTGGATCCCGTGGA
  GCCACATGGCGAGATGAAGTTTCAGTGGGACCTCAATGCCTGGACCAAGAGTGCCGAGGC
  CTTTGGCAAGATTGTGGCAGAGCGTCCTGGCACTAACTCAACAGGCCCTGCACCCATGGC
  CCCTCCAAGAGCCCCAGGGCCCCTGAGCAAGCAGGGCTCTGGCAGCAGCCAGCCCATGGA
  GGTGCAGGAAGGCTATGGCTTTGGGTCAGGAGATGATCCCTACTCAAGTGCAGAGCCCCA
  TGTGTCAGGTGTGAAACGGTCCCGCTCAGGTGAGGGCGAGGTGAGCGGCCTTATGCGCAA
  GGTGCCACGAGTGAGCCTTGAACGCCTGGACCTGGACCTCACAGCTGACAGCCAGCCACC
  CGTCTTCAAGGTCTTCCCAGGCAGTACCACTGAGGACTACAACCTTATTGTTATTGAACG
  TGGCGCTGCCGCTGCAGCTACCGGCCAGCCAGGGACTGCGCCTGCAGGAACCCCTGGTGC
  CCCACCCCTGGCTGGCATGGCCATTGTCAAGGAGGAGGAGACGGAGGCTGCCATTGGAGC
  CCCTCCTACTGCCACTGAGGGCCCTGAGACCAAACCTGTGCTTATGGCTCTTGCGGAGGG
  TCCTGGTGCTGAGGGTCCCCGCCTGGCCTCACCTAGTGGCAGCACCAGCTCAGGGCTGGA
  GGTGGTGGCTCCTGAGGGTACCTCAGCCCCAGGTGGTGGCCCGGGAACCCTGGATGACAG
  TGCCACCATTTGCCGTGTCTGCCAGAAGCCAGGCGATCTGGTTATGTGCAACCAGTGTGA
  GTTTTGTTTCCACCTGGACTGTCACCTGCCGGCCCTGCAGGATGTACCAGGGGAGGAGTG
  GAGCTGCTCACTCTGCCATGTGCTCCCTGACCTGAAGGAGGAGGATGGCAGCCTCAGCCT
  GGATGGTGCAGACAGCACTGGCGTGGTGGCCAAGCTCTCACCAGCCAACCAGCGGAAATG
  TGAGCGTGTACTGCTGGCCCTATTCTGTCACGAACCCTGCCGCCCCCTGCATCAGCTGGC
  TACCGACTCCACCTTCTCCCTGGACCAGCCCGGTGGCACCCTGGATCTGACCCTGATCCG
  TGCCCGCCTCCAGGAGAAGTTGTCACCTCCCTACAGCTCCCCACAGGAGTTTGCCCAGGA
  TGTGGGCCGCATGTTCAAGCAATTCAACAAGTTAACTGAGGACAAGGCAGACGTGCAGTC
  CATCATCGGCCTGCAGCGCTTCTTCGAGACGCGCATGAACGAGGCCTTCGGTGACACCAA
  GTTCTCTGCTGTGCTGGTGGAGCCCCCGCCGATGAGCCTGCCTGGTGCTGGCCTGAGTTC
  CCAGGAGCTGTCTGGTGGCCCTGGTGATGGCCCCTGAGGCTGGAGCCCCCATGGCCAGCC
  CAGCCTGGCTCTGTTCTCTGTCCTGTCACCCCATCCCCACTCCCCTGGTGGCCTGACTCC
  CACTCCCTGGTGGCCCCATCCCCCAGTTCCTCACGATATGGTTTTTACTTCTGTGGATTT
  AATAAAAACTTCACCAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_005762
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005762.2, NP_005753.1
• RefSeq Size: 2989 bp
• RefSeq ORF: 2508 bp
• Locus ID: 10155
• UniProt ID: Q13263
• Cytogenetics: 19q13.43
• Domains: zf-B_box, BROMO, RING, PHD, BBC
• Protein Families: Protein Kinase, Stem cell - Pluripotency, Transcription Factors
• Gene Summary: The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]