SIRT5 (NM_012241) Human Untagged Clone

CAT#: SC321579

SIRT5 (untagged)-Human sirtuin 5 (SIRT5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SIRT5
• Synonyms: SIR2L5
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012241.2 CCGGAGCGCGGTCGGGACACAGCGCCTCTAGGAGAAAGCCTGGAAGGCGCTCCGGGGGTA
  CCCAGAGCTCTTAGCGGGCCGGCAGCATGTGCGGGGCCCAAGTAAATGGAAATGTTTTCT
  AACATATAAAAACCTACAGAAGAAGAAAATAATTTTCTGGATCAAATTAGAAGTCTGTAT
  TATATTGATGTCTCCAGATTCAAATATATTAGAAAGCAGCCGTGGAGACAACCATCTTCA
  TTTTGGGAGAAATAACTAAAGCCCGCCTCAAGCATTAGAACTACAGACAAACCCTGATGC
  GACCTCTCCAGATTGTCCCAAGTCGATTGATTTCCCAGCTATATTGTGGCCTGAAGCCTC
  CAGCGTCCACACGAAACCAGATTTGCCTGAAAATGGCTCGGCCAAGTTCAAGTATGGCAG
  ATTTTCGAAAGTTTTTTGCAAAAGCAAAGCACATAGTCATCATCTCAGGAGCTGGTGTTA
  GTGCAGAAAGTGGTGTTCCGACCTTCAGAGGAGCTGGAGGTTATTGGAGAAAATGGCAAG
  CCCAGGACCTGGCGACTCCCCTGGCCTTTGCCCACAACCCGTCCCGGGTGTGGGAGTTCT
  ACCACTACCGGCGGGAGGTCATGGGGAGCAAGGAGCCCAACGCCGGGCACCGCGCCATAG
  CCGAGTGTGAGACCCGGCTGGGCAAGCAGGGCCGGCGAGTCGTGGTCATCACCCAGAACA
  TCGATGAGCTGCACCGCAAGGCTGGCACCAAGAACCTTCTGGAGATCCATGGTAGCTTAT
  TTAAAACTCGATGTACCTCTTGTGGAGTTGTGGCTGAGAATTACAAGAGTCCAATTTGTC
  CAGCTTTATCAGGAAAAGGTGCTCCAGAACCTGGAACTCAAGATGCCAGCATCCCAGTTG
  AGAAACTTCCCCGGTGTGAAGAGGCAGGCTGCGGGGGCTTGCTGCGACCTCATGTCGTGT
  GGTTTGGAGAAAACCTGGATCCTGCCATTCTGGAGGAGGTTGACAGAGAGCTCGCCCACT
  GTGATTTATGTCTAGTGGTGGGCACTTCCTCTGTGGTGTACCCAGCAGCCATGTTTGCCC
  CCCAGGTGGCTGCCAGGGGCGTGCCAGTGGCTGAATTTAACACGGAGACCACCCCAGCTA
  CGAACAGATTCAGGTTTCATTTCCAGGGACCCTGTGGAACGACTCTTCCTGAAGCCCTTG
  CCTGTCATGAAAATGAAACTGTTTCTTAAGTGTCCTGGGGAAGAAAGAAATTACAGTATA
  TCTAAGAACTAGGCCACACGCAGAGGAGAAATGGTCTTATGGGTGGTGAGCTGAGTACTG
  AACAATCTAAAAATAGCCTCTGATTCCCTCGCTGGAATCCAACCTGTTGATAAGTGATGG
  GGGTTTAGAAGTAGCAAAGAGCACCCACATTCAAAAGTCACAGAACTGGAAAGTTAATTC
  ATATTATTTGGTTTGAACTGAAACGTGAGGTATCTTTGATGTGTATGGTTGGTTATTGGG
  AGGGAAAAATTTTGTAAATTAGATTGTCTAAAAAAAATAGTTATTCTGATTATATTTTTG
  TTATCTGGGCAAAGTAGAAGTCAAGGGGTAAAAACCCTACTATTCTGATTTTTGCACAAG
  TTTTAGTGGAAAATAAAATCACACTCTACAGTAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_012241
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012241.2, NP_036373.1
• RefSeq Size: 1670 bp
• RefSeq ORF: 933 bp
• Locus ID: 23408
• UniProt ID: Q9NXA8
• Cytogenetics: 6p23
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2010]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.