PP2A-alpha (PPP2CA) (NM_002715) Human Untagged Clone

CAT#: SC321401

PPP2CA (untagged)-Human protein phosphatase 2, catalytic subunit, alpha isozyme (PPP2CA)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PP2A-alpha
• Synonyms: NEDLBA; PP2Ac; PP2CA; PP2Calpha; RP-C
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002715.2 CTCTGGAGCCTCAGCGAGCGGAGGAGGAGGCGCAGCGGCCGACGGCCGAGTACTGCGGTG
  AGAGCCAGCGGGCCAGCGCCAGCCTCAACAGCCGCCAGAAGTACACGAGGAACCGGCGGC
  GGCGTGTGCGTGTAGGCCCGTGTGCGGGCGGCGGCGCGGGAGCAGCGCGGAGCGGCAGCC
  GGCTGGGGCGGGTGGCATCATGGACGAGAAGGTGTTCACCAAGGAGCTGGACCAGTGGAT
  CGAGCAGCTGAACGAGTGCAAGCAGCTGTCCGAGTCCCAGGTCAAGAGCCTCTGCGAGAA
  GGCTAAAGAAATCCTGACAAAAGAATCCAACGTGCAAGAGGTTCGATGTCCAGTTACTGT
  CTGTGGAGATGTGCATGGGCAATTTCATGATCTCATGGAACTGTTTAGAATTGGTGGCAA
  ATCACCAGATACAAATTACTTGTTTATGGGAGATTATGTTGACAGAGGATATTATTCAGT
  TGAAACAGTTACACTGCTTGTAGCTCTTAAGGTTCGTTACCGTGAACGCATCACCATTCT
  TCGAGGGAATCATGAGAGCAGACAGATCACACAAGTTTATGGTTTCTATGATGAATGTTT
  AAGAAAATATGGAAATGCAAATGTTTGGAAATATTTTACAGATCTTTTTGACTATCTTCC
  TCTCACTGCCTTGGTGGATGGGCAGATCTTCTGTCTACATGGTGGTCTCTCGCCATCTAT
  AGATACACTGGATCATATCAGAGCACTTGATCGCCTACAAGAAGTTCCCCATGAGGGTCC
  AATGTGTGACTTGCTGTGGTCAGATCCAGATGACCGTGGTGGTTGGGGTATATCTCCTCG
  AGGAGCTGGTTACACCTTTGGGCAAGATATTTCTGAGACATTTAATCATGCCAATGGCCT
  CACGTTGGTGTCTAGAGCTCACCAGCTAGTGATGGAGGGATATAACTGGTGCCATGACCG
  GAATGTAGTAACGATTTTCAGTGCTCCAAACTATTGTTATCGTTGTGGTAACCAAGCTGC
  AATCATGGAACTTGACGATACTCTAAAATACTCTTTCTTGCAGTTTGACCCAGCACCTCG
  TAGAGGCGAGCCACATGTTACTCGTCGTACCCCAGACTACTTCCTGTAATGAAATTTTAA
  ACTTGTACAGTATTGCCATGAACCATATATCGACCTAATGGAAATGGGAAGAGCAACAGT
  AACTCCAAAGTGTCAGAAAATAGTTAACATTCAAAAAACTTGTTTTCACATGGACCAAAA
  GATGTGCCATATAAAAATACAAAGCCTCTTGTCATCAACAGCCGTGACCACTTTAGAATG
  AACCAGTTCATTGCATGCTGAAGCGACATTGTTGGTCAAGAAACCAGTTTCTGGCATAGC
  GCTATTTGTAGTTACTTTTGCTTTCTCTGAGAGACTGCAGATAATAAGATGTAAACATTA
  ACACCTCGTGAATACAATTTAACTTCCATTTAGCTATAGCTTTACTCAGCATGACTGTAG
  ATAAGGATAGCAGCAAACAATCATTGGAGCTTAATGAACATTTTTAAAAATAATTACCAA
  GGCCTCCCTTCTACTTGTGAGTTTTGAAATTGTTCTTTTTATTTTCAGGGATACCGTTTA
  ATTTAATTATATGATTTGTCTGCACTCAGTTTATTCCCTACTCAAATCTCAGCCCCATGT
  TGTTCTTTGTTATTGTCAGAACCTGGTGAGTTGTTTTGAACAGAACTGTTTTTTCCCCTT
  CCTGTAAGACGATGTGACTGCACAAGAGCACTGCAGTGTTTTTCATAATAAACTTGTGAA
  CTAAGAACTGAGAAGGTCAAATTTTAATTGTATCAATGGGCAAGACTGGTGCTGTTTATT
  AAAAAAGTTAAATCAATTGAGTAAATTTTAGAATTTGTAGACTTGTAGGTAAAATAAAAA
  TCAAGGGCACTACATAACCTCTCTGGTAACTCCTTGACATTCTTCAGATTAACTTCAGGA
  TTTATTTGTATTTCACATATTACAATTTGTCACATTGTTGGTGTGCACTTTGTGGGTTCT
  TCCTGCATATTAACTTGTTTGTAAGAAAGGAAATCTGTGCTGCTTCAGTAAGACTTAATT
  GTAAAACCATATAACTTGAGATTTAAGTCTTTGGGTTTTGTTTTAATAAAACAGCATGTT
  TTCAGGTAGAGCTTAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002715
• Insert Size: 930 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002715.2, NP_002706.1
• RefSeq Size: 2643 bp
• RefSeq ORF: 930 bp
• Locus ID: 5515
• UniProt ID: P67775
• Cytogenetics: 5q31.1
• Domains: Metallophos, PP2Ac
• Protein Families: Druggable Genome, Phosphatase, Transcription Factors
• Protein Pathways: Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway
• Gene Summary: This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.