NOLC1 (NM_004741) Human Untagged Clone

SKU SC321368

NOLC1 (untagged)-Human nucleolar and coiled-body phosphoprotein 1 (NOLC1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: NOLC1
• Synonyms: NOPP130; NOPP140; NS5ATP13; P130; Srp40
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004741.1 CTGGAGGATGGCGGACGCCGGCATTCGCCGCGTGGTTCCCAGCGACCTGTATCCCCTCGT; GCTCGGCTTCCTGCGCGATAACCAACTCTCAGAGGTGGCCAATAAGTTCGCCAAAGCGAC; AGGAGCTACACAGCAGGATGCCAATGCCTCTTCCCTCTTAGACATCTATAGCTTCTGGCT; CAACAGGTCTGCCAAGGTCCCAGAGCGAAAGTTACAGGCAAATGGACCAGTGGCTAAGAA; AGCTAAGAAGAAGGCCTCATCCAGTGACAGTGAGGACAGCAGCGAGGAGGAGGAGGAAGT; TCAAGGGCCTCCAGCAAAGAAGGCTGCTGTACCTGCCAAGCGAGTCGGTCTGCCTCCTGG; GAAGGCTGCAGCCAAAGCATCAGAGAGTAGCAGCAGTGAAGAGTCCAGTGATGATGATGA; TGAGGAGGACCAAAAGAAACAGCCTGTCCAGAAGGGAGTTAAGCCCCAAGCCAAGGCAGC; CAAAGCTCCTCCTAAGAAGGCCAAGAGCTCTGATTCTGATTCTGACTCAAGCTCCGAGGA; TGAGCCACCAAAGAACCAGAAGCCAAAGATAACACCTGTGACAGTTAAAGCTCAGACTAA; AGCCCCTCCCAAACCAGCTCGAGCAGCACCTAAAATAGCCAATGGTAAAGCAGCCAGTAG; CAGCAGTAGCAGCAGCAGCAGCAGTAGCAGTGATGACTCAGAGGAGGAGAAGGCAGCAGC; CACCCCCAAGAAGACTGTACCTAAAAAGCAAGTTGTGGCCAAGGCCCCAGTGAAAGCAGC; TACCACCCCTACCCGGAAGAGTTCTAGCAGTGAGGATTCCTCCAGTGACGAGGAAGAGGA; GCAAAAAAAACCCATGAAAAATAAACCAGGTCCCTACAGTTCAGTCCCCCCGCCTTCTGC; TCCCCCACCAAAGAAGTCTCTGGGAACCCAGCCTCCCAAGAAGGCTGTGGAGAAGCAGCA; GCCTGTGGAAAGCAGTGAAGACAGCAGTGATGAGTCTGATTCAAGTTCTGAAGAAGAGAA; GAAACCCCCAACTAAGGCAGTAGTCTCTAAAGCAACCACTAAACCACCTCCAGCAAAGAA; AGCAGCAGAGAGCTCTTCAGACAGCTCAGACTCTGACAGCTCTGAGGATGATGAAGCTCC; TTCTAAGCCAGCTGGTACCACCAAGAATTCTTCAAATAAGCCAGCTGTCACCACCAAGTC; ACCTGCAGTGAAGCCAGCTGCAGCCCCCAAGCAACCTGTGGGCGGTGGCCAGAAGCTTCT; GACGAGAAAGGCTGACAGCAGCTCCAGTGAGGAAGAGAGCAGCTCCAGTGAGGAGGAGAA; GACAAAGAAGATGGTGGCCACCACTAAGCCCAAGGCGACTGCCAAAGCAGCTCTATCTCT; GCCTGCCAAGCAGGCTCCTCAGGGTAGTAGGGACAGCAGCTCTGATTCAGACAGCTCCAG; CAGTGAGGAGGAGGAAGAGAAGACATCTAAGTCTGCAGTTAAGAAGAAGCCACAGAAGGT; AGCAGGAGGTGCAGCCCCTTCCAAGCCAGCCTCTGCAAAGAAAGGAAAGGCTGAGAGCAG; CAACAGTTCTTCTTCTGATGACTCCAGTGAGGAAGAGGAAGAGAAGCTCAAGGGCAAGGG; CTCTCCAAGACCACAAGCCCCCAAGGCCAATGGCACCTCTGCACTGACTGCCCAGAATGG; AAAAGCAGCTAAGAACAGTGAGGAGGAGGAAGAAGAAAAGAAAAAGGCGGCAGTGGTAGT; TTCCAAATCAGGTTCATTAAAGAAGCGGAAGCAGAATGAGGCTGCCAAGGAGGCAGAGAC; TCCTCAGGCCAAGAAGATAAAGCTTCAGACCCCTAACACATTTCCAAAAAGGAAGAAAGG; AGAAAAAAGGGCATCATCCCCATTCCGAAGGGTCAGGGAGGAGGAAATTGAGGTGGATTC; ACGAGTTGCGGACAACTCCTTTGATGCCAAGCGAGGTGCAGCCGGAGACTGGGGAGAGCG; AGCCAATCAGGTTTTGAAGTTCACCAAAGGCAAGTCCTTTCGGCATGAGAAAACCAAGAA; GAAGCGGGGCAGCTACCGGGGAGGCTCAATCTCTGTCCAGGTCAATTCTATTAAGTTTGA; CAGCGAGTGACCTGAGGCCATCTTCGGTGAAGCAAGGGTGATGATCGGAGACTACTTACT; TTCTCCAGTGGACCTGGGAACCCTCAGGTCTCTAGGTGAGGGTCTTGATGAGGACAGAAG; TTTAGAGTAGGTCCTAAGACTTTACAGTGTAACATCCTCTCTGGTCCTTTTCTGTGTTCC; TAGTTTTGTACAGACTTGTTTTTGAGTGTTGAGTAGCAGGGACAAAATAAGGGAATGTTA; TTTTTTAAGAAAATTCATTTTCATTGTTGTCTCCTTCCTTTTCTGTGAAAGTCCTCATAC; TGAGAAATTTGTATATTTTATATTAAATCACTTACTATTGAAAAAAAAAAAAAAAAAAAA; AAAA
• Restriction Sites: Please inquire
• ACCN: NM_004741
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004741.1, NP_004732.1
• RefSeq Size: 2502 bp
• RefSeq ORF: 2100 bp
• Locus ID: 9221
• UniProt ID: Q14978
• Cytogenetics: 10q24.32
• Domains: SRP40_C
• Protein Families: Stem cell - Pluripotency
• Summary: Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (PubMed:10567578, PubMed:26399832). Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832). Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus (PubMed:9016786). It has intrinsic GTPase and ATPase activities (PubMed:9016786).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.