CD43 (SPN) (NM_003123) Human Untagged Clone

CAT#: SC320913

SPN (untagged)-Human sialophorin (SPN), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD43
• Synonyms: CD43; GALGP; GPL115; LSN
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003123.3 CTTCCCTGCCTCCCTCAGGTCCCAGCTCTTGCTCCTGCCTGTTTGCCTGGAAATGGCCAC
  GCTTCTCCTTCTCCTTGGGGTGCTGGTGGTAAGCCCAGACGCTCTGGGGAGCACAACAGC
  AGTGCAGACACCCACCTCCGGAGAGCCTTTGGTCTCTACTAGCGAGCCCCTGAGCTCAAA
  GATGTACACCACTTCAATAACAAGTGACCCTAAGGCCGACAGCACTGGGGACCAGACCTC
  AGCCCTACCTCCCTCAACTTCCATCAATGAGGGATCCCCTCTTTGGACTTCCATTGGTGC
  CAGCACTGGTTCCCCTTTACCTGAGCCAACAACCTACCAGGAAGTTTCCATCAAGATGTC
  ATCAGTGCCCCAGGAAACCCCTCATGCAACCAGTCATCCTGCTGTTCCCATAACAGCAAA
  CTCTCTAGGATCCCACACCGTGACAGGTGGAACCATAACAACGAACTCTCCAGAAACCTC
  CAGTAGGACCAGTGGAGCCCCTGTTACCACGGCAGCTAGCTCTCTGGAGACCTCCAGAGG
  CACCTCTGGACCCCCTCTTACCATGGCAACTGTCTCTCTGGAGACTTCCAAAGGCACCTC
  TGGACCCCCTGTTACCATGGCAACTGACTCTCTGGAGACCTCCACTGGGACCACTGGACC
  CCCTGTTACCATGACAACTGGCTCTCTGGAGCCCTCCAGCGGGGCCAGTGGACCCCAGGT
  CTCTAGCGTAAAACTATCTACAATGATGTCTCCAACGACCTCCACCAACGCAAGCACTGT
  GCCCTTCCGGAACCCAGATGAGAACTCACGAGGCATGCTGCCAGTGGCTGTGCTTGTGGC
  CCTGCTGGCGGTCATAGTCCTCGTGGCTCTGCTCCTGCTGTGGCGCCGGCGGCAGAAGCG
  GCGGACTGGGGCCCTCGTGCTGAGCAGAGGCGGCAAGCGTAACGGGGTGGTGGACGCCTG
  GGCTGGGCCAGCCCAGGTCCCTGAGGAGGGGGCCGTGACAGTGACCGTGGGAGGGTCCGG
  GGGCGACAAGGGCTCTGGGTTCCCCGATGGGGAGGGGTCTAGCCGTCGGCCCACGCTCAC
  CACTTTCTTTGGCAGACGGAAGTCTCGCCAGGGCTCCCTGGCGATGGAGGAGCTGAAGTC
  TGGGTCAGGCCCCAGCCTCAAAGGGGAGGAGGAGCCACTGGTGGCCAGTGAGGATGGGGC
  TGTGGACGCCCCAGCTCCTGATGAGCCCGAAGGGGGAGACGGGGCTGCCCCTTAAGTGTC
  GGTGAATAGTGAGGCTGGAGGCCGGAATCTCAGCCAGCCTCCAGCACCTTCCCTCTCACC
  ATCCCACTGCCCCCTCGCTCCCATGTTTCCACCCGGCACCCTGATCCTCACCCGAATCTC
  CTTTTTTTTTTTCTTTTGAGACAGAGTTTCGCTTTGTCGCCCAGGCTGGAGTGCAATGCA
  CGATCTCAGTTCACTGCAACCTCTGCCTCCTAAGTTCAGGCGATTCTCCTGCCTCAGCTT
  CCCGAGTAACTGAGATTACAGGCACCCACCACCATGCCCAGCTGCTTTTTTGTATTTTTG
  GTAGAGATGGGGTTTCACCATGTTGGCTAGGCTGGTCTCAAACTCCTGACCTCAGGTGAT
  CTACCTGCCTCAGCCTCCCAAAGTGCTGAGATTACAGACATGAGCCTCCGCGCCTTGCCT
  CCTCACCCACCTCTTCACTCTGAATCCTCATGAGGCTTCTCAGCCCTGGATTTCCTGCTG
  CCATCCTCACCCAGCACCCACAACTAGCGCCTGGGCAGGGCAGGGCTGGCACCTCTCAAC
  GTCTGTGGACTGAATGAATAAACCCTCCTCATCCACCCCTAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_003123
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003123.3, NP_003114.1
• RefSeq Size: 6871 bp
• RefSeq ORF: 1203 bp
• Locus ID: 6693
• UniProt ID: P16150
• Cytogenetics: 16p11.2
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• Gene Summary: This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation, and has one potential N-glycosylation site, while the carboxy-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome. [provided by RefSeq, Sep 2017]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.