GRB10 (NM_001001555) Human Untagged Clone

SKU SC320797

GRB10 (untagged)-Human growth factor receptor-bound protein 10 (GRB10), transcript variant 4

Product Data

• Type: Human Untagged Clone
• Target Symbol: GRB10
• Synonyms: Grb-10; GRB-IR; IRBP; MEG1; RSS
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001001555.1 GAGCCGCGCGCGGCAGCTTTGGCGCTGACCACAATGCTGAGCAGGAAGCAGCAGCTGCAG; GCCCAGTGACTGGTAGCTCAGTGACCAGCAGCCCAGTGACCGGCAGCCAGGTCCTCACCT; GGGTCCTCTCAGTGAAGCCAGGGTGGCCGCCCCAGCAGACAGTGCTACAGAGCCAACTCC; TGACAGGACAAGGTGGAGCAGACACCTCGCAGTCAACAAGACCCGGCAGGACCAGGACTC; CCCGCACAGTCTGACCGACTTGCGAATCACCAGGAGGATGATGTGGACCTGGAAGCCCTG; GTGAACGATATGAATGCATCCCTGGAGAGCCTGTACTCGGCCTGCAGCATGCAGTCAGAC; ACGGTGCCCCTCCTGCAGAATGGCCAGCATGCCCGCAGCCAGCCTCGGGCTTCAGGCCCT; CCTCGGTCCATCCAGCCACAGGTGTCCCCAAGGCAGAGGGTGCAGCGCTCCCAGCCTGTG; CACATCCTCGCTGTCAGGCGCCTTCAGGAGGAAGACCAGCAGTTTAGAACCTCATCTCTG; CCGGCCATCCCCAATCCTTTTCCTGAACTCTGTGGCCCTGGGAGCCCCCCTGTGCTCACG; CCGGGTTCTTTACCTCCGAGCCAGGCCGCCGCAAAGCAGGATGTTAAAGTCTTTAGTGAA; GATGGGACAAGCAAAGTGGTGGAGATTCTAGCAGACATGACAGCCAGAGACCTGTGCCAA; TTGCTGGTTTACAAAAGTCACTGTGTGGATGACAACAGCTGGACACTAGTGGAGCACCAC; CCGCACCTAGGATTAGAGAGGTGCTTGGAAGACCATGAGCTGGTGGTCCAGGTGGAGAGT; ACCATGGCCAGTGAGAGTAAATTTCTATTCAGGAAGAATTACGCAAAATACGAGTTCTTT; AAAAATCCCATGAATTTCTTCCCAGAACAGATGGTTACTTGGTGCCAGCAGTCAAATGGC; AGTCAAACCCAGCTTTTGCAGAATTTTCTGAACTCCAGTAGTTGTCCTGAAATTCAAGGG; TTTTTGCATGTGAAAGAGCTGGGAAAGAAATCATGGAAAAAGCTGTATGTGTGTTTGCGG; AGATCTGGCCTTTATTGCTCCACCAAGGGAACTTCAAAGGAACCCAGACACCTGCAGCTG; CTGGCCGACCTGGAGGACAGCAACATCTTCTCCCTGATCGCTGGCAGGAAGCAGTACAAC; GCCCCTACAGACCACGGGCTCTGCATAAAGCCAAACAAAGTCAGGAATGAAACTAAAGAG; CTGAGGTTGCTCTGTGCAGAGGACGAGCAAACCAGGACGTGCTGGATGACAGCGTTCAGA; CTCCTCAAGTATGGAATGCTCCTTTACCAGAATTACCGAATCCCTCAGCAGAGGAAGGCC; TTGCTGTCCCCGTTCTCGACGCCAGTGCGCAGTGTCTCCGAGAACTCCCTCGTGGCAATG; GATTTTTCTGGGCAAACAGGACGCGTGATAGAGAATCCGGCGGAGGCCCAGAGCGCAGCC; CTGGAGGAGGGCCACGCCTGGAGGAAGCGAAGCACACGGATGAACATCCTAGGTAGCCAA; AGTCCCCTCCACCCTTCTACCCTAAGTACAGTGATTCACAGGACACAGCACTGGTTTCAC; GGGAGGATCTCCAGGGAGGAATCCCACAGGATCATTAAACAGCAAGGGCTCGTGGATGGG; CTTTTTCTCCTCCGTGACAGCCAGAGTAATCCAAAGGCATTTGTACTCACACTGTGTCAT; CACCAGAAAATTAAAAATTTCCAGATCTTACCTTGCGAGGACGACGGGCAGACGTTCTTC; AGCCTAGATGACGGGAACACCAAATTCTCTGACCTGATCCAGCTGGTTGACTTTTACCAG; CTGAACAAAGGAGTCCTGCCTTGCAAACTCAAGCACCACTGCATCCGAGTGGCCTTATGA; CCGCAGATGTCCTCTCGGCTGAAGACTGGAGGAAGTGAACACTGGAGTGAAGAAGCGGTC; TGTGCGTTGGTGAAGAACACACATCGATTCTGCACCTGGGGACCCAGAGCGAGATGGGTT; TGTTCGGTGCCAGCCGACCAAGATTGACTAGTTTGTTGGACTTAAACGACGATTTGCTGC; TGTGAACCCAGCAGGGTCGCCTCCCTCTGCGTCGGCCAAATTGGGGAGGGCATGGAAGAT; CCAGCGGAAAGTTGAAAATAAACTGGAATGATCATCTTGGCTTGGGCCGCTTAGGAACAA; GAACCGGAGAGAAGTGATTGGAAATGAACTCTTGCCCTGGAATAATCTTGACAATTAAAA; CTGATATGTTTAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001001555
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001001555.1, NP_001001555.1
• RefSeq Size: 5043 bp
• RefSeq ORF: 1611 bp
• Locus ID: 2887
• UniProt ID: Q13322
• Cytogenetics: 7p12.1
• Protein Families: Druggable Genome
• Summary: The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2010]; Transcript Variant: This variant (4) differs in the 5' UTR and the 5' coding region, compared to variant 1. The resulting isoform (c) has a shorter N-terminus when compared to isoform a. Isoform c is encoded by transcript variants 3 and 4.