HDGF (NM_004494) Human Untagged Clone

SKU SC320791

HDGF (untagged)-Human hepatoma-derived growth factor (HDGF), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: HDGF
• Synonyms: HMG1L2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004494 edited; ACGAGGGCGCGGGCGGAGACGCCGTGGCCGCGCCGGAGCTCGGGCCGGGGGCCACCATCG; AGGCGGGGGCCGCGCGAGGGCCGGAGCGGAGCGGCGCCGCCACCGCCGCACGCGCAAACT; TGGGCTCGCGCTTCCCGGCCCGGCGCGGAGCCCGGGGCGCCCGGAGCCCCGCCATGTCGC; GATCCAACCGGCAGAAGGAGTACAAATGCGGGGACCTGGTGTTCGCCAAGATGAAGGGCT; ACCCACACTGGCCGGCCCGGATTGACGAGATGCCTGAGGCTGCCGTGAAATCAACAGCCA; ACAAATACCAAGTCTTTTTTTTCGGGACCCACGAGACGGCATTCCTGGGCCCCAAAGACC; TCTTCCCTTACGAGGAATCCAAGGAGAAGTTTGGCAAGCCCAACAAGAGGAAAGGGTTCA; GCGAGGGGCTGTGGGAGATCGAAAACAACCCTACTGTCAAGGCTTCCGGCTATCAGTCCT; CCCAGAAAAAGAGCTGTGTGGAAGAGCCTGAACCAGAGCCCGAAGCTGCAGAGGGTGACG; GTGATAAGAAGGGGAATGCAGAGGGCAGCAGCGACGAGGAAGGGAAGCTGGTCATTGATG; AGCCAGCCAAGGAGAAGAACGAGAAAGGAGCGTTGAAGAGGAGAGCAGGGGACTTGCTGG; AGGACTCTCCTAAACGTCCCAAGGAGGCAGAAAACCCTGAAGGAGAGGAGAAGGAGGCAG; CCACCTTGGAGGTTGAGAGGCCCCTTCCTATGGAGGTGGAAAAGAATAGCACCCCCTCTG; AGCCCGGCTCTGGCCGGGGGCCTCCCCAAGAGGAAGAAGAAGAGGAGGATGAAGAGGAAG; AGGCTACCAAGGAAGATGCTGAGGCCCCAGGCATCAGAGATCATGAGAGCCTGTAGCCAC; CAATGTTTCAAGAGGAGCCCCCACCCTGTTCCTGCTGCTGTCTGGGTGCTACTGGGGAAA; CTGGCCATGGCCTGCAAACTGGGAACCCCTTTCCCACCCCAACCTGCTCTCCTCTTCTAC; TCACTTTTCCCACTCCAAGCCCAGCCCATGGAGATTGACCTGGATGGGGCAGGCCACCTG; GCTCTCACCTCTAGGTCCCCATACTCCTATGATCTGAGTCAGAGCCATGTCTTCTCCCTG; GAATGAGTTGAGGCCACTGTGTTCCTTCCGCTTGGGAGGGGCAATCCTCAAATGCGGGGT; GGGGGCAGCACAGGAGGGCGGCCTCCTTCTGAGCTCCTGTCCCCTGCTACACCTATTATC; CCAGCTGCCTAGATTCAGGGAAAGTGGGACAGCTTGTAGGGGAGGGGCTCCTTTCCATAA; ATCCTTGATGATTGACAACACCCATTTTTCCTTTTGCCGACCCCAAGAGTTTTGGGAGTT; GTAGTTAATCATCAAGAGAATTTGGGGCTTCCAAGTTGTTCGGGCCAAGGACCTGAGACC; TGAAGGGTTGACTTTACCCATTTGGGTGGGAGTGTTGAGCATCTGTCCCCCTTTAGATCT; CTGAAGCCACAAATAGGATGCTTGGGAAGACTCCTAGCTGTCCTTTTTCCTCTCCACACA; GTGCTCAAGGCCAGCTTATAGTCATATATATCACCCAGACATAAAGGAAAAGACACATTT; TTTAGGAAATGTTTTTAATAAAAGAAAATTACAAAAAAAAATTTTAAAGACCCCTAACCC; TTTGTGTGCTCTCCATTCTGCTCCTTCCCCATCGTTGCCCCCATTTCTGAGGTGCACTGG; GAGGCTCCCCTTCTATTTGGGGCTTGATGACTTTCTTTTTGTAGCTGGGGCTTTGATGTT; CCTTCCAGTGTCATTTCTCATCCACATACCCTGACCTGGCCCCCTCAGTGTTGTCACCAG; ATCTGATTTGTAACCCACTGAGAGGACAGAGAGAAATAAGTGCCCTCTCCCACCCTCTTC; CTACTGGTCTCTCTATGCCTCTCTACAGTCTCGTCTCTTTTACCCTGGCCCCTCTCCCTT; GGGCTCTGATGAAAAATTGCTGACTGTAGCTTTGGAAGTTTAGCTCTGAGAACCGTAGAT; GATTTCAGTTCTAGGAAAATAAAACCCGTTGATTACTAAAAAAAAAAAAAAAAAAA;
• Restriction Sites: Please inquire
• ACCN: NM_004494
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004494.1, NP_004485.1
• RefSeq Size: 2376 bp
• RefSeq ORF: 723 bp
• Locus ID: 3068
• UniProt ID: P51858
• Cytogenetics: 1q23.1
• Domains: PWWP
• Summary: This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. This gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2016]; Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence and uses an alternate in-frame splice junction compared to variant 4. The resulting isoform (a) has a shorter and distinct N-terminus and lacks an alternate internal segment compared to isoform d.