BAP1 (NM_004656) Human Untagged Clone

CAT#: SC320669

BAP1 (untagged)-Human BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) (BAP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BAP1
• Synonyms: hucep-6; HUCEP-13; UCHL2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004656.2 GCCCGTTGTCTGTGTGTGGGACTGAGGGGCCCCGGGGGCGGTGGGGGCTCCCGGTGGGGG
  CAGCGGTGGGGAGGGAGGGCCTGGACATGGCGCTGAGGGGCCGCCCCGCGGGAAGATGAA
  TAAGGGCTGGCTGGAGCTGGAGAGCGACCCAGGCCTCTTCACCCTGCTCGTGGAAGATTT
  CGGTGTCAAGGGGGTGCAAGTGGAGGAGATCTACGACCTTCAGAGCAAATGTCAGGGCCC
  TGTATATGGATTTATCTTCCTGTTCAAATGGATCGAAGAGCGCCGGTCCCGGCGAAAGGT
  CTCTACCTTGGTGGATGATACGTCCGTGATTGATGATGATATTGTGAATAACATGTTCTT
  TGCCCACCAGCTGATACCCAACTCTTGTGCAACTCATGCCTTGCTGAGCGTGCTCCTGAA
  CTGCAGCAGCGTGGACCTGGGACCCACCCTGAGTCGCATGAAGGACTTCACCAAGGGTTT
  TAGCCCTGAGAGCAAAGGATATGCGATTGGCAATGCCCCGGAGTTGGCCAAGGCCCATAA
  TAGCCATGCCAGGCCCGAGCCACGCCACCTCCCTGAGAAGCAGAATGGCCTTAGTGCAGT
  GCGGACCATGGAGGCGTTCCACTTTGTCAGCTATGTGCCTATCACAGGCCGGCTCTTTGA
  GCTGGATGGGCTGAAGGTCTACCCCATTGACCATGGGCCCTGGGGGGAGGACGAGGAGTG
  GACAGACAAGGCCCGGCGGGTCATCATGGAGCGTATCGGCCTCGCCACTGCAGGGGAGCC
  CTACCACGACATCCGCTTCAACCTGATGGCAGTGGTGCCCGACCGCAGGATCAAGTATGA
  GGCCAGGCTGCATGTGCTGAAGGTGAACCGTCAGACAGTACTAGAGGCTCTGCAGCAGCT
  GATAAGAGTAACACAGCCAGAGCTGATTCAGACCCACAAGTCTCAAGAGTCACAGCTGCC
  TGAGGAGTCCAAGTCAGCCAGCAACAAGTCCCCGCTGGTGCTGGAAGCAAACAGGGCCCC
  TGCAGCCTCTGAGGGCAACCACACAGATGGTGCAGAGGAGGCGGCTGGTTCATGCGCACA
  AGCCCCATCCCACAGCCCTCCCAACAAACCCAAGCTAGTGGTGAAGCCTCCAGGCAGCAG
  CCTCAATGGGGTTCACCCCAACCCCACTCCCATTGTCCAGCGGCTGCCGGCCTTTCTAGA
  CAATCACAATTATGCCAAGTCCCCCATGCAGGAGGAAGAAGACCTGGCGGCAGGTGTGGG
  CCGCAGCCGAGTTCCAGTCCGCCCACCCCAGCAGTACTCAGATGATGAGGATGACTATGA
  GGATGACGAGGAGGATGACGTGCAGAACACCAACTCTGCCCTTAGGTATAAGGGGAAGGG
  AACAGGGAAGCCAGGGGCATTGAGCGGTTCTGCTGATGGGCAACTGTCAGTGCTGCAGCC
  CAACACCATCAACGTCTTGGCTGAGAAGCTCAAAGAGTCCCAGAAGGACCTCTCAATTCC
  TCTGTCCATCAAGACTAGCAGCGGGGCTGGGAGTCCGGCTGTGGCAGTGCCCACACACTC
  GCAGCCCTCACCCACCCCCAGCAATGAGAGTACAGACACGGCCTCTGAGATCGGCAGTGC
  TTTCAACTCGCCACTGCGCTCGCCTATCCGCTCAGCCAACCCGACGCGGCCCTCCAGCCC
  TGTCACCTCCCACATCTCCAAGGTGCTTTTTGGAGAGGATGACAGCCTGCTGCGTGTTGA
  CTGCATACGCTACAACCGTGCTGTCCGTGATCTGGGTCCTGTCATCAGCACAGGCCTGCT
  GCACCTGGCTGAGGATGGGGTGCTGAGTCCCCTGGCGCTGACAGAGGGTGGGAAGGGTTC
  CTCGCCCTCCATCAGACCAATCCAAGGCAGCCAGGGGTCCAGCAGCCCAGTGGAGAAGGA
  GGTCGTGGAAGCCACGGACAGCAGAGAGAAGACGGGGATGGTGAGGCCTGGCGAGCCCTT
  GAGTGGGGAGAAATACTCACCCAAGGAGCTGCTGGCACTGCTGAAGTGTGTGGAGGCTGA
  GATTGCAAACTATGAGGCGTGCCTCAAGGAGGAGGTAGAGAAGAGGAAGAAGTTCAAGAT
  TGATGACCAGAGAAGGACCCACAACTACGATGAGTTCATCTGCACCTTTATCTCCATGCT
  GGCTCAGGAAGGCATGCTGGCCAACCTAGTGGAGCAGAACATCTCCGTGCGGCGGCGCCA
  AGGGGTCAGCATCGGCCGGCTCCACAAGCAGCGGAAGCCTGACCGGCGGAAACGCTCTCG
  CCCCTACAAGGCCAAGCGCCAGTGAGGACTGCTGGCCCTGACTCTGCAGCCCACTCTTGC
  CGTGTGGCCCTCACCAGGGTCCTTCCCTGCCCCACTTCCCCTTTTCCCAGTATTACTGAA
  TAGTCCCAGCTGGAGAGTCCAGGCCCTGGGAATGGGAGGAACCAGGCCACATTCCTTCCA
  TCGTGCCCTGAGGCCTGACACGGCAGATCAGCCCCATAGTGCTCAGGAGGCAGCATCTGG
  AGTTGGGGCACAGCGAGGTACTGCAGCTTCCTCCACAGCCGGCTGTGGAGCAGCAGGACC
  TGGCCCTTCTGCCTGGGCAGCAGAATATATATTTTACCTATCAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004656
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004656.2, NP_004647.1
• RefSeq Size: 3599 bp
• RefSeq ORF: 2190 bp
• Locus ID: 8314
• UniProt ID: Q92560
• Cytogenetics: 3p21.1
• Domains: Peptidase_C12
• Protein Families: Druggable Genome, Protease
• Gene Summary: This gene belongs to the ubiquitin C-terminal hydrolase subfamily of deubiquitinating enzymes that are involved in the removal of ubiquitin from proteins. The encoded enzyme binds to the breast cancer type 1 susceptibility protein (BRCA1) via the RING finger domain of the latter and acts as a tumor suppressor. In addition, the enzyme may be involved in regulation of transcription, regulation of cell cycle and growth, response to DNA damage and chromatin dynamics. Germline mutations in this gene may be associated with tumor predisposition syndrome (TPDS), which involves increased risk of cancers including malignant mesothelioma, uveal melanoma and cutaneous melanoma. [provided by RefSeq, May 2013]