14-3-3 epsilon (YWHAE) (NM_006761) Human Untagged Clone

CAT#: SC320619

YWHAE (untagged)-Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (YWHAE), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: 14-3-3 epsilon
• Synonyms: 14-3-3E; HEL2; KCIP-1; MDCR; MDS
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006761.3 CGGAGCGAGAGGCTGAGAGAGTCGGAGACACTATCCGCTTCCATCCGTCGCGCAGACCCT
  GCCGGAGCCGCTGCCGCTATGGATGATCGAGAGGATCTGGTGTACCAGGCGAAGCTGGCC
  GAGCAGGCTGAGCGATACGACGAAATGGTGGAGTCAATGAAGAAAGTAGCAGGGATGGAT
  GTGGAGCTGACAGTTGAAGAAAGAAACCTCCTATCTGTTGCATATAAGAATGTGATTGGA
  GCTAGAAGAGCCTCCTGGAGAATAATCAGCAGCATTGAACAGAAAGAAGAAAACAAGGGA
  GGAGAAGACAAGCTAAAAATGATTCGGGAATATCGGCAAATGGTTGAGACTGAGCTAAAG
  TTAATCTGTTGTGACATTCTGGATGTACTGGACAAACACCTCATTCCAGCAGCTAACACT
  GGCGAGTCCAAGGTTTTCTATTATAAAATGAAAGGGGACTACCACAGGTATCTGGCAGAA
  TTTGCCACAGGAAACGACAGGAAGGAGGCTGCGGAGAACAGCCTAGTGGCTTATAAAGCT
  GCTAGTGATATTGCAATGACAGAACTTCCACCAACGCATCCTATTCGCTTAGGTCTTGCT
  CTCAATTTTTCCGTATTCTACTACGAAATTCTTAATTCCCCTGACCGTGCCTGCAGGTTG
  GCAAAAGCAGCTTTTGATGATGCAATTGCAGAACTGGATACGCTGAGTGAAGAAAGCTAT
  AAGGACTCTACACTTATCATGCAGTTGTTACGTGATAATCTGACACTATGGACTTCAGAC
  ATGCAGGGTGACGGTGAAGAGCAGAATAAAGAAGCGCTGCAGGACGTGGAAGACGAAAAT
  CAGTGAGACATAAGCCAACAAGAGAAACCATCTCTGACCACCCCCTCCTCCCCATCCCAC
  CCTTTGGAAACTCCCCATTGTCACTGAGAACCACCAAATCTGACTTTTACATTTGGTCTC
  AGAATTTAGGTTCCTGCCCTGTTGGTTTTTTTTTTTTTTTTTTTAAACAGTTTTCAAAAG
  TTCTTAAAGGCAAGAGTGAATTTCTGTGGATTTTACTGGTCCCAGCTTTTAGGTTCTTTA
  AGACACTAACAGGACTACATAGAGGCTTTTTCAGCATTACTGTGTCGTCTCCGTGCCAGA
  TGTGGCAAGATCACCATTAGCAAATGGAAATTACATTTGAAAGCCATTAGACTTACAGGT
  GATGCAAGCATCTAAGAGAGAGGTTAATCACACTATAGAGGCATAAGTGGTATCAGTTTT
  CATTTTTCTAATTGTTTAAACTGTGTTTTATACCAGTGTTTGCAAGTAATTGGGTGTTAG
  CTTGAGATGGTTAAAGGTGGTTTGGGGAGGGACTTCGTTGTAATGGTTTTGCTGTAAAAA
  ATGTTTCCAACTCCGCTGAAATGTTGCTGAAAAGCATGGTGCTGGTAACAGTTCAACAAT
  CCGTGGCTGCTCATTCTTGCCTACTTTACTCTCCCACTGAAGCAGGTTAGCGTTGAAGGT
  GGTATGGAAAAGCCTGCATGCCTGTTCAATTCTTTTGTTTCTTCTCCTTCCCCCTCCCCC
  TACCTCCTTCCCCTCACTCCTCCCCTCCTTCGCTCGCTCAACCTCTTTTGTTCAGTATGT
  GTAACTTGAAGCTAATTTGTACTACTGGATATCTGACTGGAGCCACAGATACAGAATCTG
  TATTGTTCTTACTGAAACACAGCATGGAATTAACATTAAACTTAAATAAAACAAACCTAA
  ATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_006761
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006761.3, NP_006752.1
• RefSeq Size: 1810 bp
• RefSeq ORF: 768 bp
• Locus ID: 7531
• UniProt ID: P62258
• Cytogenetics: 17p13.3
• Domains: 14-3-3
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis
• Gene Summary: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]
  Transcript Variant: This variant (1) represents the protein-coding transcript.