14-3-3 epsilon (YWHAE) (NM_006761) Human Untagged Clone

SKU SC112695

YWHAE (untagged)-Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (YWHAE), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: 14-3-3 epsilon
• Synonyms: 14-3-3E; HEL2; KCIP-1; MDCR; MDS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112695 sequence for NM_006761 edited (data generated by NextGen Sequencing); ATGGATGATCGAGAGGATCTGGTGTACCAGGCGAAGCTGGCCGAGCAGGCTGAGCGATAC; GACGAAATGGTGGAGTCAATGAAGAAAGTAGCAGGGATGGATGTGGAGCTGACAGTTGAA; GAAAGAAACCTCCTATCTGTTGCATATAAGAATGTGATTGGAGCTAGAAGAGCCTCCTGG; AGAATAATCAGCAGCATTGAACAGAAAGAAGAAAACAAGGGAGGAGAAGACAAGCTAAAA; ATGATTCGGGAATATCGGCAAATGGTTGAGACTGAGCTAAAGTTAATCTGTTGTGACATT; CTGGATGTACTGGACAAACACCTCATTCCAGCAGCTAACACTGGCGAGTCCAAGGTTTTC; TATTATAAAATGAAAGGGGACTACCACAGGTATCTGGCAGAATTTGCCACAGGAAACGAC; AGGAAGGAGGCTGCGGAGAACAGCCTAGTGGCTTATAAAGCTGCTAGTGATATTGCAATG; ACAGAACTTCCACCAACGCATCCTATTCGCTTAGGTCTTGCTCTCAATTTTTCCGTATTC; TACTACGAAATTCTTAATTCCCCTGACCGTGCCTGCAGGTTGGCAAAAGCAGCTTTTGAT; GATGCAATTGCAGAACTGGATACGCTGAGTGAAGAAAGCTATAAGGACTCTACACTTATC; ATGCAGTTGTTACGTGATAATCTGACACTATGGACTTCAGACATGCAGGGTGACGGTGAA; GAGCAGAATAAAGAAGCGCTGCAGGACGTGGAAGACGAAAATCAGTGA; ; Clone variation with respect to NM_006761.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006761 unedited; GGCGAATTTTGTAATACGACTCACTATTAGGGCGGCCGCGATTCGGCACGAGGTCGGAGA; CACTATCCGCTTCCATCCGTCGCGCAGACCCTGCCGGAGCCGCTGCCGCTATGGATGATC; GAGAGGATCTGGTGTACCAGGCGAAGCTGGCCGAGCAGGCTGAGCGATACGACGAAATGG; TGGAGTCAATGAAGAAAGTAGCAGGGATGGATGTGGAGCTGACAGTTGAAGAAAGAAACC; TCCTATCTGTTGCATATAAGAATGTGATTGGAGCTAGAAGAGCCTCCTGGAGAATAATCA; GCAGCATTGAACAGAAAGAAGAAAACAAGGGAGGAGAAGACAAGCTAAAAATGATTCGGG; AATATCGGCAAATGGTTGAGACTGAGCTAAAGTTAATCTGTTGTGACATTCTGGATGTAC; TGGACAAACACCTCATTCCAGCAGCTAACACTGGCGAGTCCAAGGTTTTCTATTATAAAA; TGAAAGGGGACTACCACAGGTATCTGGCAGAATTTGCCACAGGAAACGACAGGAAGGAGG; CTGCGGAGAACAGCCTAGTGGCTTATAAAGCTGCTAGTGATATTGCAATGACAGAACTTC; CACCAACGCATCCTATTCGCTTAGGTCTTGCTCTCAATTNTTCCGTATTCTACTACGAAA; TTCTTAATTCCCCTGACCGTGCCTGCAGGTTGGCAAAAGCAGCTTTTGATGATGNCATTG; CAGAACTGGATACGCTGAGTGAAGAAAGCTATTAGGACTCTACACTTATCATGCAGTTGN; TACGTGATAATCTGACACTATGGACTTCAGACTGCAGGGTGACGGTGAAGAGCANAATAA; AGAGCGCTGCAGGACGTGGAAGACGAAAATCAGTGAGACATAAGCCCACAGAGAAACATC; TCTGACCCCCCCTTCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006761 unedited; GCGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTAATTTAGGTTT; GTTTTATTTAAGTTTAATGTTAATTCCATGCTGGGTTTCAGTAAGAACAATACAGATTCT; GTATCTGTGGCTCCAGTCAGATATCCAGTAGTACAAATTAGCTTCAAGTTACACATACTG; AACAAAAGAGGTTGAGCGAGCGAAGGAGGGGAGGAGTGAGGGGAAGGAGGTAGGGGGAGG; GGGNAANGCCCCNNCCCNNNNNNNTTGNNCNNNCNTGCNNNCTTTTCCNTNCCNCCTTCN; NCGCTTCCCTGCTTTNTTGNCCNNGCCCCGTGGGTCTGCTTGCGCGGCCCCGGNTTGTTC; GTCTGTGTCCNCTNCCTTGCTTTCTGTCCCCCTTGCTTCGGCGGTGCTTTCGTGTTTTTC; GCCGGNCCGTTGCCTCGTCGCCCTTTCTCGNGCTTCCTTGCCCCGTTGGCTTCTTTCGCC; CCTTGTCTTGCCTGGCGGCCTGGCCCCCCGGCCTCCCCTTTCTGNTCCTTGCCTCCTGCT; TCCCCTTTGCCTCTTTCGGTCGTTCCCCCGTTTCTCCCTGGGGCTTCTTNTGTGGTCTTT; TGGCTCGCGCCCGTTCTCTCCCTTCGCTGTTGTTCCCTTTGCTCCTTTGTCCCGGGTTTG; ATCGCTCTCTGTTTNCCCCGCTCTGTTTTTTCTGCCTTCGCCTCTTGCCTCTTTCGCTGT; GCCCCGCNTGTCTTCCGTGGTCCCTCTTGCTTCTGCGCTCTCGTCTCTTTGTCTCTCCCC; TTGTGTCTCTCCCGCCGCNTTTCTTTCTTCCCCTTCTCCTCTTTTTTTGTCCTCTCCTTT; CTTTGGTCTCCGGCTGGCTGCTTCTCCCGCTGGTGCCCTGCNTTCGCCCGTACTGGTTAC; TCTTGGTTCAGGTCTCCCCTCTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_006761
• Insert Size: 1770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006761.3, NP_006752.1
• RefSeq Size: 1810 bp
• RefSeq ORF: 768 bp
• Locus ID: 7531
• UniProt ID: P62258
• Cytogenetics: 17p13.3
• Domains: 14-3-3
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis
• Summary: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]; Transcript Variant: This variant (1) represents the protein-coding transcript.