NFX1 (NM_002504) Human Untagged Clone

SKU SC320406

NFX1 (untagged)-Human nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: NFX1
• Synonyms: NFX2; TEG-42; Tex42
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002504.3 GCTCGATCTAGGTTCTGCGGCACGGGATGGCGGAGGCGCCTCCTGTCTCAGGTACTTTTA; AATTCAATACAGATGCTGCTGAATTCATTCCTCAGGAGAAAAAAAATTCTGGTCTAAATT; GTGGGACTCAAAGGAGACTAGACTCTAATAGGATTGGTAGAAGAAATTACAGTTCACCAC; CTCCCTGTCACCTTTCCAGGCAGGTCCCTTATGATGAAATCTCTGCTGTTCATCAGCATA; GTTATCATCCGTCAGGAAGCAAACCTAAGAGTCAGCAGACGTCTTTCCAGTCCTCTCCTT; GTAATAAATCGCCCAAGAGCCATGGCCTTCAGAATCAACCTTGGCAGAAATTGAGGAATG; AGAAGCACCATATCAGAGTCAAGAAAGCACAGAGTCTTGCTGAGCAGACCTCAGATACAG; CTGGATTAGAGAGCTCGACCAGATCAGAGAGTGGGACAGACCTCAGAGAGCATAGTCCTT; CTGAGAGTGAGAAGGAAGTTGTGGGTGCAGATCCCAGGGGAGCAAAACCCAAAAAAGCAA; CACAGTTTGTATACAGCTATGGTAGAGGACCAAAAGTCAAGGGGAAACTCAAATGTGAAT; GGAGTAACCGAACAACTCCAAAACCGGAGGATGCTGGACCCGAAAGTACCAAACCTGTGG; GGGTTTTCCACCCTGACTCTTCAGAGGCATCCTCTAGAAAAGGAGTATTGGATGGGTATG; GAGCCAGACGAAATGAGCAGAGAAGATACCCACAGAAAAGGCCTCCCTGGGAAGTGGAGG; GGGCCAGGCCACGACCAGGCAGAAATCCACCAAAACAGGAGGGCCACCGACATACAAACG; CAGGACACAGAAACAACATGGGCCCCATTCCAAAGGATGACCTCAATGAAAGACCAGCAA; AATCTACCTGTGACAGTGAGAACTTGGCAGTCATCAACAAGTCTTCCAGGAGGGTTGACC; AAGAGAAATGCACTGTACGGAGGCAGGATCCTCAAGTAGTATCTCCTTTCTCCCGAGGCA; AACAGAACCATGTGCTAAAGAATGTGGAAACGCACACAGGTTCTCTAATTGAACAACTAA; CAACAGAAAAATACGAGTGCATGGTGTGCTGTGAATTGGTTCGTGTCACGGCCCCAGTGT; GGAGTTGTCAGAGCTGTTACCATGTGTTTCATTTGAACTGCATAAAGAAATGGGCAAGGT; CTCCAGCATCTCAAGCAGATGGCCAGAGTGGTTGGAGGTGCCCTGCCTGTCAGAATGTTT; CTGCACATGTTCCTAATACCTACACTTGTTTCTGTGGCAAGGTAAAGAATCCTGAGTGGA; GCAGAAATGAAATTCCACATAGCTGTGGTGAGGTTTGTAGAAAGAAACAGCCTGGCCAGG; ACTGCCCACATTCCTGTAACCTTCTCTGCCATCCAGGACCCTGCCCACCCTGCCCTGCCT; TTATGACAAAAACATGTGAATGTGGACGAACCAGGCACACAGTTCGCTGTGGTCAGGCTG; TCTCAGTCCACTGTTCTAACCCATGTGAGAATATTTTGAACTGTGGTCAGCACCAGTGTG; CTGAGCTGTGCCATGGGGGTCAGTGCCAGCCTTGCCAGATCATTTTGAACCAGGTATGCT; ATTGCGGCAGCACCTCCCGAGATGTGTTATGTGGAACCGATGTAGGAAAGTCTGATGGAT; TTGGGGATTTCAGCTGTTTAAAGATATGTGGCAAGGACTTGAAATGCGGTAACCATACAT; GTTCGCAAGTGTGCCACCCTCAGCCCTGCCAGCAATGCCCACGGCTCCCCCAGCTGGTGC; GCTGTTGCCCCTGTGGCCAAACTCCTCTCAGCCAATTGCTAGAACTTGGAAGTAGTAGTC; GGAAAACATGCATGGACCCTGTGCCTTCATGTGGAAAAGTGTGCGGCAAGCCTCTGCCTT; GTGGTTCCTTAGATTTCATTCATACCTGTGAAAAGCTCTGCCATGAAGGAGACTGTGGAC; CATGCTCTCGCACATCAGTTATTTCCTGCAGATGCTCTTTCAGAACAAAGGAGCTTCCAT; GTACCAGTCTCAAAAGTGAAGATGCTACATTTATGTGTGACAAGCGGTGTAACAAGAAAC; GGTTGTGTGGACGGCATAAATGTAATGAGATATGCTGTGTGGATAAGGAGCACAAGTGTC; CTTTGATTTGTGGGAGGAAACTCCGTTGTGGCCTTCATAGGTGTGAAGAACCTTGTCATC; GTGGAAACTGCCAGACATGCTGGCAAGCCAGTTTTGATGAATTAACCTGCCATTGTGGTG; CATCAGTGATTTACCCTCCAGTTCCCTGTGGTACTAGGCCCCCTGAATGTACCCAAACCT; GCGCTAGAGTCCATGAGTGTGACCATCCAGTATATCATTCTTGTCATAGTGAGGAGAAGT; GTCCCCCTTGCACTTTCCTAACTCAGAAGTGGTGCATGGGCAAGCATGAGTTTCGGAGCA; ACATCCCCTGTCACCTGGTTGATATCTCTTGCGGATTACCCTGCAGTGCCACGCTACCAT; GTGGGATGCACAAATGTCAGAGACTCTGTCACAAAGGGGAGTGTCTTGTGGATGAGCCCT; GCAAGCAGCCCTGCACCACCCCCAGAGCTGACTGTGGTCACCCGTGTATGGCACCCTGCC; ATACCAGCTCACCCTGCCCTGTGACTGCTTGTAAAGCTAAGGTAGAGCTACAGTGTGAAT; GTGGACGAAGAAAAGAGATGGTGATTTGCTCTGAAGCATCTAGTACTTATCAAAGAATAG; CTGCAATCTCCATGGCCTCTAAGATAACAGACATGCAGCTTGGAGGTTCAGTGGAGATCA; GCAAGTTAATTACCAAAAAGGAAGTTCATCAAGCCAGGCTGGAGTGTGATGAGGAGTGTT; CAGCCTTGGAAAGGAAAAAGAGATTAGCAGAGGCATTTCATATCAGTGAGGATTCTGATC; CTTTCAATATACGTTCTTCAGGGTCAAAATTCAGTGATAGTTTGAAAGAAGATGCCAGGA; AGGACTTAAAGTTTGTCAGTGACGTTGAGAAGGAAATGGAAACCCTCGTGGAGGCCGTGA; ATAAGGGAAAGAATAGTAAGAAAAGCCACAGCTTCCCTCCCATGAACAGAGACCACCGCC; GGATCATCCATGACTTGGCCCAAGTTTATGGCCTGGAGAGCGTGAGCTATGACAGTGAAC; CGAAGCGCAATGTGGTGGTCACTGCCATCAGGGGGAAGTCCGTTTGTCCTCCTACCACGC; TGACAGGTGTGCTTGAAAGGGAAATGCAGGCACGGCCTCCACCACCGATTCCTCATCACA; GACATCAGTCAGACAAGAATCCTGGGAGCAGTAATTTACAGAAAATAACCAAGGAGCCAA; TAATTGACTATTTTGACGTCCAGGACTAAGAAGATCATGATGCACTTAGATAAAAGAATG; ATTAGGTATAGTGGAGACTTATTTGCCAGCAGATAAATCATGCCCGTTCCCCTCTGCCTG; GCAGAATCACAGTCTCACATACTGTCTTGTACTGACACATCCAAAGCATGAGTGTGTCAG; AAATCCCTTGTCTATTCCTGTCTGTATAAAGTGTTTCATTATGACCAGATCTCTGATTGT; ATGGTCACTAGGTATGCAATCACGCATTCAAAGAGGCTCTTTACACCATCACTGTGATTG; CTCTGAGAGTTGAGGGACTATTGGGCTTTATTTGGACAAACCAAACTTTTAGCCTGAAAC; CAACTTTATGCCACTAAGTCATAGCCTCAGTTGTCCCAGTTATTTGTCCTCCTGAAAATG; CCTGAAACATCAGACAGACATTGCTTGCTTTACCCAAACTGATCAAAATCTTTAGGAGCA; CAAATGAATTTTTTAGTCTGAAATACCAAATAATGAATTGGTATACCATATCCGGAATCA; CACATGTTATCTTAAACCCAGCCATCATACCTAAGTCTTTTGCCAAAACCTCTCATAGGT; ATATCTAGCTGAACTTATTTTGGCATTTTCAATGTGATCAGTTCTAGACCTAGAAGGGGG; TCAGGCTGCTTTACAGAATTCTATTTCCTTAAGTCCCTGGCACTTCTCATACCACATCAC; TGAACCTGTTCAGTAACAATCAGTTTGGCCGTCCCCCATGATGGTAGGAAATATAGAGAG; CAAGTTCTTCTGCCAGGGTCACACTGTGGTCTCTGAACTGACCAGTATATCCCTAACTCC; TCTTTGATAGAGAAAGAGTCTCAAATGGACAACTGTCCTGTGTTGCTTTCCCTAGGCCTT; CAGCAGCCTATTGGCTCTCCCTGCCTCTGAGCTCTGGACTCTGTTTGAATATTCCAAGTA; GTATATGGACAGTCCAGGGCTTATGCCCAGCAGCCCACTGGAGGCATTCTTCAGGCTCCT; TTAAGGCAGGTGCATTGATAGTTCCATTAGTGTGACCCTTGCATTGGCACCCCTCCAGCC; TGGAGGCCAGGCTTCCAGCAACTTCCTTCTGCCCTAGAGCAAGCCATGAGCCCCAGAGCA; GTAGCAGGAGACTTGAGAAGTAGAGTGACAAAAACAAGCACTTAATTAAATTATAAAATT; TAACTTTAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002504
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002504.3, NP_002495.2
• RefSeq Size: 4629 bp
• RefSeq ORF: 3363 bp
• Locus ID: 4799
• UniProt ID: Q12986
• Cytogenetics: 9p13.3
• Domains: R3H, zf-NF-X1
• Protein Families: Druggable Genome, Transcription Factors
• Summary: MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. The protein encoded by this gene is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma. Three alternative splice variants, each of which encodes a different isoform, have been identified. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) encodes isoform 1.