CAF1 (CHAF1A) (NM_005483) Human Untagged Clone

CAT#: SC319958

CHAF1A (untagged)-Human chromatin assembly factor 1, subunit A (p150) (CHAF1A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CAF1
• Synonyms: CAF-1; CAF1; CAF1B; CAF1P150; P150
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005483.2 GGCGGCAGCAGCGGCGCGGGCGGGAGGGCGAAGAGCAGCGGCCGCCTGAGGGGAGCCCGC
  GCCTCCGCCGCCTGAGAGGAGGTCGAGCTGCCGCCGGGGCGATGCTGGAGGAGCTGGAGT
  GCGGGGCGCCCGGCGCCAGGGGAGCCGCCACAGCCATGGATTGCAAAGATAGACCAGCTT
  TTCCAGTTAAGAAGTTAATACAAGCCCGTCTGCCGTTTAAGCGCCTGAATCTTGTCCCAA
  AGGGGAAAGCCGATGACATGTCAGACGATCAGGGTACTTCTGTGCAAAGTAAAAGCCCCG
  ATTTAGAGGCCTCTTTGGACACCTTGGAAAACAACTGTCATGTGGGTTCTGACATAGACT
  TTAGACCGAAACTTGTCAACGGGAAGGGTCCCTTAGATAACTTTTTAAGAAATAGAATCG
  AAACCAGTATTGGCCAGAGCACAGTCATCATTGATTTGACAGAGGACTCGAATGAGCAGC
  CAGACAGTCTTGTGGACCACAATAAACTAAATTCTGAAGCCTCTCCCTCCAGGGAGGCAA
  TAAATGGCCAGCGAGAAGACACTGGGGATCAGCAGGGGTTGTTGAAGGCCATTCAGAACG
  ACAAGTTGGCATTTCCTGGAGAGACCCTTTCAGACATTCCTTGCAAAACAGAGGAGGAGG
  GTGTTGGCTGTGGAGGTGCAGGGAGGAGAGGCGACTCCCAGGAATGTTCGCCACGGAGCT
  GCCCGGAGCTGACGAGTGGCCCGAGAATGTGCCCCAGAAAGGAGCAGGACAGTTGGAGTG
  AAGCTGGGGGCATCCTGTTCAAAGGGAAGGTGCCTATGGTGGTCTTGCAGGACATCTTGG
  CTGTGAGACCACCGCAAATCAAGTCCCTTCCAGCCACACCCCAAGGCAAGAACATGACCC
  CTGAGAGTGAGGTGCTGGAATCTTTCCCCGAAGAAGACTCTGTACTCAGCCATTCGTCCC
  TGAGCTCTCCCTCTTCCACCAGCTCGCCCGAGGGGCCGCCTGCTCCCCCAAAGCAGCACA
  GCAGTACCAGTCCCTTCCCCACCTCCACGCCCCTCCGCAGAATAACTAAGAAATTCGTCA
  AAGGCTCTACAGAGAAGAACAAGCTCAGACTGCAAAGAGATCAGGAGCGTCTGGGCAAGC
  AGCTCAAGTTACGTGCAGAAAGGGAAGAAAAGGAGAAGCTGAAAGAGGAGGCCAAGCGGG
  CCAAGGAGGAGGCCAAGAAGAAGAAGGAGGAAGAGAAGGAGCTTAAGGAAAAGGAGAGGC
  GGGAGAAGCGGGAGAAGGATGAGAAGGAGAAGGCGGAGAAGCAGCGGCTCAAGGAGGAGC
  GGCGCAAGGAGAGACAGGAAGCCCTGGAGGCTAAACTTGAGGAAAAAAGGAAAAAGGAAG
  AAGAGAAACGGTTAAGAGAAGAAGAGAAGCGCATTAAAGCAGAGAAGGCCGAAATCACGA
  GGTTCTTCCAGAAACCAAAGACTCCACAGGCCCCCAAGACCCTGGCCGGCTCCTGTGGGA
  AGTTTGCCCCCTTTGAAATTAAAGAGCACATGGTCCTGGCCCCTCGGCGTCGGACCGCTT
  TCCATCCAGACCTCTGCAGTCAGCTGGACCAGCTCCTCCAGCAGCAGAGCGGCGAGTTCT
  CCTTCTTGAAAGACCTCAAAGGCCGGCAGCCCCTGAGGTCCGGACCCACGCACGTTTCCA
  CCCGGAATGCAGATATTTTTAACAGTGATGTCGTCATCGTGGAGCGTGGGAAGGGCGACG
  GTGTTCCCGAGAGGAGGAAGTTTGGCAGGATGAAGCTCCTGCAGTTCTGTGAGAACCACC
  GGCCTGCCTACTGGGGTACCTGGAATAAGAAGACGGCACTCATCCGCGCGCGAGACCCCT
  GGGCCCAGGACACGAAGCTCCTGGACTATGAGGTGGACAGTGATGAGGAGTGGGAAGAAG
  AGGAGCCTGGGGAGTCCCTGTCCCACAGTGAGGGGGATGATGATGACGACATGGGAGAGG
  ATGAAGATGAGGACGATGGTTTCTTTGTGCCCCATGGGTACCTGTCTGAGGACGAAGGTG
  TGACAGAGGAGTGTGCCGACCCTGAGAACCATAAGGTCCGCCAGAAACTGAAGGCCAAGG
  AGTGGGACGAGTTCCTGGCTAAGGGGAAGCGCTTTCGCGTCCTGCAACCTGTGAAGATCG
  GCTGCGTGTGGGCGGCTGACAGAGACTGCGCAGGCGATGACCTGAAGGTACTGCAGCAGT
  TCGCAGCCTGCTTCCTGGAGACCCTGCCGGCCCAGGAGGAGCAGACGCCCAAGGCCTCCA
  AGCGGGAGAGGAGAGACGAGCAGATCCTGGCCCAGCTGCTGCCGCTCCTGCACGGCAATG
  TGAACGGGAGCAAGGTCATCATCCGGGAGTTCCAGGAGCACTGCCGCCGGGGACTGCTCA
  GCAACCACACCGGCAGCCCGCGGAGCCCCTCCACCACCTACCTGCACACCCCCACCCCCA
  GCGAGGATGCCGCCATCCCCTCTAAGTCCCGGCTCAAGCGGCTCATTTCCGAGAACTCAG
  TGTATGAGAAGCGGCCTGACTTCAGGATGTGCTGGTACGTGCACCCGCAGGTGCTACAGA
  GCTTCCAGCAGGAGCACCTGCCCGTGCCGTGCCAGTGGAGCTATGTGACATCGGTGCCCT
  CGGCCCCCAAAGAGGACAGTGGCAGCGTCCCCTCCACGGGGCCCAGCCAGGGCACTCCCA
  TCTCGCTGAAGAGGAAGTCAGCGGGCAGCATGTGCATCACCCAATTCATGAAGAAGCGCA
  GGCACGACGGCCAGATTGGTGCTGAAGACATGGACGGCTTCCAGGCAGACACGGAGGAGG
  AGGAAGAGGAGGAGGGCGACTGTATGATCGTGGATGTCCCGGATGCTGCGGAGGTCCAAG
  CCCCGTGTGGAGCCGCTTCCGGAGCTGGGGGTGGTGTGGGGGTGGACACCGGCAAGGCCA
  CCCTGACCGCGAGCCCACTGGGTGCATCCTGAGAGCAGGGGTGACGTATGTAGAATGCTT
  AGGGTGTCCTCCCCACAGAGCAGATACTTGAACCGACTCAATTCCTGTGTAAAGAGCACT
  TTGTCCTGCTTCACGGACCTCCCCAAAGTGTGCAGAGTTCTATATAGGATGCTGGATTAG
  TTCCTTTGATATTTGTAAAAATTCCCCCAAGAGCCGCATATGAATCTGCCCTTTAATAAA
  GCATTATTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_005483
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005483.2, NP_005474.2
• RefSeq Size: 3344 bp
• RefSeq ORF: 2871 bp
• Locus ID: 10036
• UniProt ID: Q13111
• Cytogenetics: 19p13.3
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Gene Summary: Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).[supplied by OMIM, Mar 2008]