PLD4 (NM_138790) Human Untagged Clone

SKU
SC319634
PLD4 (untagged)-Human phospholipase D family, member 4 (PLD4)
$472.00
In Stock*
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PLD4
Synonyms C14orf175
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_138790.2 GGAGGTTGGTGTGGAGCACAGGCAGCACCGAGCCTGCCCCGTGAGCTGAGGGCCTGCAGT
CTGCGGCTGGAATCAGGATAGACACCAAGGCAGGACCCCCAGAGATGCTGAAGCCTCTTT
GGAAAGCAGCAGTGGCCCCCACATGGCCATGCTCCATGCCGCCCCGCCGCCCGTGGGACA
GAGAGGCTGGCACGTTGCAGGTCCTGGGAGCGCTGGCTGTGCTGTGGCTGGGCTCCGTGG
CTCTTATCTGCCTCCTGTGGCAAGTGCCCCGTCCTCCCACCTGGGGCCAGGTGCAGCCCA
AGGACGTGCCCAGGTCCTGGGAGCATGGCTCCAGCCCAGCTTGGGAGCCCCTGGAAGCAG
AGGCCAGGCAGCAGAGGGACTCCTGCCAGCTTGTCCTTGTGGAAAGCATCCCCCAGGACC
TGCCATCTGCAGCCGGCAGCCCCTCTGCCCAGCCTCTGGGCCAGGCCTGGCTGCAGCTGC
TGGACACTGCCCAGGAGAGCGTCCACGTGGCTTCATACTACTGGTCCCTCACAGGGCCTG
ACATCGGGGTCAACGACTCGTCTTCCCAGCTGGGAGAGGCTCTTCTGCAGAAGCTGCAGC
AGCTGCTGGGCAGGAACATTTCCCTGGCTGTGGCCACCAGCAGCCCGACACTGGCCAGGA
CATCCACCGACCTGCAGGTTCTGGCTGCCCGAGGTGCCCATGTACGACAGGTGCCCATGG
GGCGGCTCACCAGGGGTGTTTTGCACTCCAAATTCTGGGTTGTGGATGGACGGCACATAT
ACATGGGCAGTGCCAACATGGACTGGCGGTCTCTGACGCAGGTGAAGGAGCTTGGCGCTG
TCATCTATAACTGCAGCCACCTGGCCCAAGACCTGGAGAAGACCTTCCAGACCTACTGGG
TACTGGGGGTGCCCAAGGCTGTCCTCCCCAAAACCTGGCCTCAGAACTTCTCATCTCACT
TCAACCGTTTCCAGCCCTTCCACGGCCTCTTTGATGGGGTGCCCACCACTGCCTACTTCT
CAGCGTCGCCACCAGCACTCTGTCCCCAGGGCCGCACCCGGGACCTGGAGGCGCTGCTGG
CGGTGATGGGGAGCGCCCAGGAGTTCATCTATGCCTCCGTGATGGAGTATTTCCCCACCA
CGCGCTTCAGCCACCCCCCGAGGTACTGGCCGGTGCTGGACAACGCGCTGCGGGCGGCAG
CCTTCGGCAAGGGCGTGCGCGTGCGCCTGCTGGTCGGCTGCGGACTCAACACGGACCCCA
CCATGTTCCCCTACCTGCGGTCCCTGCAGGCGCTCAGCAACCCCGCGGCCAACGTCTCTG
TGGACGTGAAAGTCTTCATCGTGCCGGTGGGGAACCATTCCAACATCCCATTCAGCAGGG
TGAACCACAGCAAGTTCATGGTCACGGAGAAGGCAGCCTACATAGGCACCTCCAACTGGT
CGGAGGATTACTTCAGCAGCACGGCGGGGGTGGGCTTGGTGGTCACCCAGAGCCCTGGCG
CGCAGCCCGCGGGGGCCACGGTGCAGGAGCAGCTGCGGCAGCTCTTTGAGCGGGACTGGA
GTTCGCGCTACGCCGTCGGCCTGGACGGACAGGCTCCGGGCCAGGACTGCGTTTGGCAGG
GCTGAGGGGGGCCTCTTTTTCTCTCGGCGACCCCGCCCCGCACGCGCCCTCCCCTCTGAC
CCCGGCCTGGGCTTCAGCCGCTTCCTCCCGCAAGCAGCCCGGGTCCGCACTGCGCCAGGA
GCCGCCTGCGACCGCCCGGGCGTCGCAAACCGCCCGCCTGCTCTCTGATTTCCGAGTCCA
GCCCCCCCTGAGCCCCACCTCCTCCAGGGAGCCCTCCAGGAAGCCCCTTCCCTGACTCCT
GGCCCACAGGCCAGGCCTAAAAAAAACTCGTGGCTTCAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAA
Restriction Sites Please inquire
ACCN NM_138790
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138790.2, NP_620145.2
RefSeq Size 1964 bp
RefSeq ORF 1521 bp
Locus ID 122618
UniProt ID Q96BZ4
Cytogenetics 14q32.33
Domains PLDc
Protein Families Transmembrane
Summary 5'->3' DNA exonuclease which digests single-stranded DNA (ssDNA). Regulates inflammatory cytokine responses via the degradation of nucleic acids, by reducing the concentration of ssDNA able to stimulate TLR9, a nucleotide-sensing receptor. Involved in phagocytosis of activated microglia.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate splice site in the 5' coding region that results in use of a downstream start codon compared to variant 1. It encodes isoform 2, which is shorter than isoform 1.
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.