SIRT7 (NM_016538) Human Untagged Clone

CAT#: SC319613

SIRT7 (untagged)-Human sirtuin 7 (SIRT7)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SIRT7
• Synonyms: SIR2L7
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_016538.1 GTCTCCAGGGGAGCGATGGCAGCCGGGGGTCTGAGCCGCTCCGAGCGCAAAGCGGCGGAG
  CGGGTCCGGAGGTTGCGGGAGGAGCAGCAGAGGGAGCGCCTCCGCCAGGTGTCGCGCATC
  CTGAGGAAGGCGGCGGCGGAGCGCAGCGCCGAGGAGGGCCGGCTGCTGGCCGAGAGCGCG
  GACCTGGTAACGGAGCTGCAGGGCCGGAGCCGGCGGCGCGAGGGCCTGAAGCGGCGGCAG
  GAGGAGGTGTGCGACGACCCGGAGGAGCTGCGGGGGAAGGTCCGGGAGCTGGCCAGCGCC
  GTCCGGAACGCCAAATACTTGGTCGTCTACACAGGCGCGGGAATCAGCACGGCAGCGTCT
  ATCCCAGACTACCGGGGCCCTAATGGAGTGTGGACACTGCTTCAGAAAGGGAGAAGCGTT
  AGTGCTGCCGACCTGAGCGAGGCCGAGCCAACCCTCACCCACATGAGCATCACCCGTCTG
  CATGAGCAGAAGCTGGTGCAGCATGTGGTGTCTCAGAACTGTGACGGGCTCCACCTGAGG
  AGTGGGCTGCCGCGCACGGCCATCTCCGAGCTCCACGGGAACATGTACATTGAAGTCTGT
  ACCTCCTGCGTTCCCAACAGGGAGTACGTGCGGGTGTTCGATGTGACGGAGCGCACTGCC
  CTCCACAGACACCAGACAGGCCGGACCTGCCACAAGTGTGGGACCCAGCTGCGGGACACC
  ATTGTGCACTTTGGGGAGAGGGGGACGTTGGGGCAGCCTCTGAACTGGGAAGCGGCGACC
  GAGGCTGCCAGCAGAGCAGACACCATCCTGTGTCTAGGGTCCAGCCTGAAGGTTCTAAAG
  AAGTACCCACGCCTCTGGTGCATGACCAAGCCCCCTAGCCGGCGGCCGAAGCTTTACATC
  GTGAACCTGCAGTGGACCCCGAAGGATGACTGGGCTGCCCTGAAGCTACATGGGAAGTGT
  GATGACGTCATGCGGCTCCTCATGGCCGAGCTGGGCTTGGAGATCCCCGCCTATAGCAGG
  TGGCAGGATCCCATTTTCTCACTGGCGACTCCCCTGCGTGCTGGTGAAGAAGGCAGCCAC
  AGTCGGAAGTCGCTGTGCAGAAGCAGAGAGGAGGCCCCGCCTGGGGACCGGGGTGCACCG
  CTTAGCTCGGCCCCCATCCTAGGGGGCTGGTTTGGCAGGGGCTGCACAAAACGCACAAAA
  AGGAAGAAAGTGACGTAATCACGTGCTCGATGAAGAACAGTTGGCACTTTGCAGATGGCC
  AGTGTCACGGTGAAGGCTGGGTTGCCCCCACGGGTCTAGGGAGAACGAACTCTTTGGGGA
  TGACATTTTCACCGTGACATTTTTAGCCATTTGTCCTTGAGGAAGCCCCTTGCACTGCTG
  CGGTTGTACCCTGATACGGCCTGGCCATCGAGGACACCTGCCCATCCGGCCTCTGTGTCA
  AGAGGTGGCAGCCGCACCTTTCTGTGAGAACGGAACTCGGGTTATTTCAGCCCCGGCCTG
  CAGAGTGGAAGCGCCCAGCGGCCTTTCCTCGCTCACCAGGCCAGTCTCAGGGCCTCACCG
  TATTTCTACTACTACTTAATGAAAAAGTGTGAACTTTATAGAATCCTCTCTGTACTGGAT
  GTGCGGCAGAGGGGTGGCTCCGAGCCTCGGCTCTATGCAGACCTTTTTATTTCTATTAAA
  CGTTTCTGCACTGGCAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_016538
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016538.1, NP_057622.1
• RefSeq Size: 1718 bp
• RefSeq ORF: 1203 bp
• Locus ID: 51547
• UniProt ID: Q9NRC8
• Cytogenetics: 17q25.3
• Domains: SIR2
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq, Jul 2008]