E2F4 (NM_001950) Human Untagged Clone

CAT#: SC319576

E2F4 (untagged)-Human E2F transcription factor 4, p107/p130-binding (E2F4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: E2F4
• Synonyms: E2F-4
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001950.3 GCGGCGGGCGGGCGCGATGGCGGAGGCCGGGCCACAGGCGCCGCCGCCCCCGGGCACTCC
  AAGCCGGCACGAAAAGAGCCTGGGACTGCTCACCACCAAGTTCGTGTCCCTTCTGCAGGA
  GGCCAAGGACGGCGTGCTTGACCTCAAGCTGGCAGCTGACACCCTAGCTGTACGCCAGAA
  GCGGCGGATTTACGACATTACCAATGTTTTGGAAGGTATCGGGCTAATCGAGAAAAAGTC
  CAAGAACAGCATCCAGTGGAAGGGTGTGGGGCCTGGCTGCAATACCCGGGAGATTGCTGA
  CAAACTGATTGAGCTCAAGGCAGAGATCGAGGAGCTGCAGCAGCGGGAGCAAGAACTAGA
  CCAGCACAAGGTGTGGGTGCAGCAGAGCATCCGGAACGTCACAGAGGACGTGCAGAACAG
  CTGTTTGGCCTACGTCACTCATGAGGACATCTGCAGATGCTTTGCTGGAGATACCCTCTT
  GGCCATCCGGGCCCCATCAGGCACCAGCCTGGAGGTGCCCATCCCAGAGGGTCTCAATGG
  GCAGAAGAAGTACCAGATTCACCTGAAGAGTGTGAGTGGTCCCATTGAGGTTCTGCTGGT
  GAACAAGGAGGCATGGAGCTCACCCCCTGTGGCTGTGCCTGTGCCACCACCTGAAGATTT
  GCTCCAGAGCCCATCTGCTGTTTCTACACCTCCACCTCTGCCCAAGCCTGCCCTAGCCCA
  GTCCCAGGAAGCCTCACGTCCAAATAGTCCTCAGCTCACTCCCACTGCTGTCCCTGGCAG
  TGCAGAAGTCCAGGGAATGGCTGGCCCAGCAGCTGAGATCACAGTGAGTGGCGGCCCTGG
  GACTGATAGCAAGGACAGTGGTGAGCTCAGTTCACTCCCACTGGGCCCAACAACACTGGA
  CACCCGGCCACTGCAGTCTTCTGCCCTGCTGGACAGCAGCAGCAGCAGCAGCAGCAGCAG
  CAGCAGCAGCAGCAACAGTAACAGCAGCAGTTCGTCCGGACCCAACCCTTCTACCTCCTT
  TGAGCCCATCAAGGCAGACCCCACAGGTGTTTTGGAACTCCCCAAAGAGCTGTCAGAAAT
  CTTTGATCCCACACGAGAGTGCATGAGCTCGGAGCTGCTGGAGGAGTTGATGTCCTCAGA
  AGTGTTTGCCCCTCTGCTTCGTCTTTCTCCACCCCCGGGAGACCACGATTATATCTACAA
  CCTGGACGAGAGTGAAGGTGTCTGTGACCTCTTTGATGTGCCTGTTCTCAACCTCTGACT
  GACAGGGACATGCCCTGTGTGGCTGGGACCCAGACTGTCTGACCTGGGGGTTGCCTGGGG
  ACCTCTCCCACCCGACCCCTACAGAGCTTGAGAGCCACAGACGCCTGGCTTCTCCGGCCT
  CCCCTCACCGCACAGTTCTGGCCACAGCTCCCGCTCCTTCCTGGCACTTCTGTGCTCGCA
  GAGCAGGGGAACAGGACTCAGCCCCCATCACCGTGGAGCCAAAGTGTTTGCTTCTCCCTT
  TCTGCGGCCTTCGCCAGCCCAGGCTCGGCTGCCACCCAGTGGCACAGAACCGAGGAGCTG
  CCATTACCCCCCATAGGGGGCAGTGTCTTGTTCCTGCCAGCCTCAGTGTCTTGCTTCTGC
  CAGCTCCTTCCCCTAGGAGGGAAGGGTGGGGTGGAACTGGGCACATGCCAGCACCACTTC
  TAGCTTCCTTCGCTATCCCCCACCCCCTGACCCTCCAGCTCCTCCTGGCCCTCTCACGTG
  CCCACTTCTGCTGGGCCTTTAGCCCTAGAACCTGCAGGTGGTGGGGGCGGCTACCAAGAA
  GGAACAGAGGTCTCTGGGGAGGAGTCTGGGTGGTCCAGCCCTGATGATTGGCCCCACCTC
  CTGCTGCCCCATAACCCTCTCTTCATTTCGGCTTTTTCATTTACCCTCATTTAGAGCCAT
  TTGCAGAGATTTAGAAAGATTTACAGTAACGAATGGATTCCTATATAAAGATTATTTTTA
  TACTTTTTGCAGCAAAAGGAAATTGTAATATTTGTACAGTGTTCAAGTGAATAAAAACCA
  TGCCTAAGGCTAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001950
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001950.3, NP_001941.2
• RefSeq Size: 2100 bp
• RefSeq ORF: 1242 bp
• Locus ID: 1874
• UniProt ID: Q16254
• Cytogenetics: 16q22.1
• Domains: E2F_TDP
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cell cycle, TGF-beta signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. [provided by RefSeq, Jul 2008]