YTHDF2 (NM_016258) Human Untagged Clone

CAT#: SC319366

YTHDF2 (untagged)-Human YTH domain family, member 2 (YTHDF2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: YTHDF2
• Synonyms: CAHL; DF2; HGRG8; NY-REN-2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_016258.2 GGCCGGCCTCTCCCTTCCCGGGGTTCTTCGCGCCGGGCCCCTTCCGCGTGGGTGAGTGAA
  TGTGAGAGTCAGCGCTCGCGCCGCGCGCGCCGCCCGCCTCCGCTGTTCGGCGCTCTGCTT
  TAGGCGGTGGGGGGCGGGCGCGCGCGTAAAAGCATAGAGACGGGCATTGAGCTCTTGGGC
  TAGAGCGTCGCCGAGTCGGAGCCGGAGCCTGAGCCGCGCGCTGTGTCTCCGCTGCGTCCG
  CCGAGGCCCCCGAGTGTCAGGGACAAAAGCCTCCGCCTGCTCCCGCAGCCGGGGCTCATC
  TGCCGCCGCCGCCGCGCTGAGGAGAGTTCGCCGCCGTCGCCGCCCGTGAGGATCTGAGAG
  CCATGTCGGCCAGCAGCCTCTTGGAGCAGAGACCAAAAGGTCAAGGAAACAAAGTACAAA
  ATGGATCTGTACATCAAAAGGATGGATTAAACGATGATGATTTTGAACCTTACTTGAGTC
  CACAGGCAAGGCCCAATAATGCATATACTGCCATGTCAGATTCCTACTTACCCAGTTACT
  ACAGTCCCTCCATTGGCTTCTCCTATTCTTTGGGTGAAGCTGCTTGGTCTACGGGGGGTG
  ACACAGCCATGCCCTACTTAACTTCTTATGGACAGCTGAGCAACGGAGAGCCCCACTTCC
  TACCAGATGCAATGTTTGGGCAACCAGGAGCCCTAGGTAGCACTCCATTTCTTGGTCAGC
  ATGGTTTTAATTTCTTTCCCAGTGGGATTGACTTCTCAGCATGGGGAAATAACAGTTCTC
  AGGGACAGTCTACTCAGAGCTCTGGATATAGTAGCAATTATGCTTATGCACCTAGCTCCT
  TAGGTGGAGCCATGATTGATGGACAGTCAGCTTTTGCCAATGAGACCCTCAATAAGGCTC
  CTGGCATGAATACTATAGACCAAGGGATGGCAGCACTGAAGTTGGGTAGCACAGAAGTTG
  CAAGCAATGTTCCAAAAGTTGTAGGTTCTGCTGTTGGTAGCGGGTCCATTACTAGTAACA
  TCGTGGCTTCCAATAGTTTGCCTCCAGCCACCATTGCTCCTCCAAAACCAGCATCTTGGG
  CTGATATTGCTAGCAAGCCTGCAAAACAGCAACCTAAACTGAAGACCAAGAATGGCATTG
  CAGGGTCAAGTCTTCCGCCACCCCCGATAAAGCATAACATGGATATTGGAACTTGGGATA
  ACAAGGGTCCCGTTGCAAAAGCCCCCTCACAGGCTTTGGTTCAGAATATAGGTCAGCCAA
  CCCAGGGGTCTCCTCAGCCTGTAGGTCAGCAGGCTAACAATAGCCCACCAGTGGCTCAGG
  CATCAGTAGGGCAACAGACACAGCCATTGCCTCCACCTCCACCACAGCCTGCCCAGCTTT
  CAGTCCAGCAACAGGCAGCTCAGCCAACCCGCTGGGTAGCACCTCGGAACCGTGGCAGTG
  GGTTCGGTCATAATGGGGTGGATGGTAATGGAGTAGGACAGTCTCAGGCTGGTTCTGGAT
  CTACTCCTTCAGAACCCCACCCAGTGTTGGAGAAGCTTCGGTCCATTAATAACTATAACC
  CCAAAGATTTTGACTGGAATCTGAAACATGGCCGGGTTTTCATCATTAAGAGCTACTCTG
  AGGACGATATTCACCGTTCCATTAAGTATAATATTTGGTGCAGCACAGAGCATGGTAACA
  AGAGACTGGATGCTGCTTATCGTTCCATGAACGGGAAAGGCCCCGTTTACTTACTTTTCA
  GTGTCAACGGCAGTGGACACTTCTGTGGCGTGGCAGAAATGAAATCTGCTGTGGACTACA
  ACACATGTGCAGGTGTGTGGTCCCAGGACAAATGGAAGGGTCGTTTTGATGTCAGGTGGA
  TTTTTGTGAAGGACGTTCCCAATAGCCAACTGCGACACATTCGCCTAGAGAACAACGAGA
  ATAAACCAGTGACCAACTCTAGGGACACTCAGGAAGTGCCTCTGGAAAAGGCTAAGCAGG
  TGTTGAAAATTATAGCCAGCTACAAGCACACCACTTCCATTTTTGATGACTTCTCACACT
  ATGAGAAACGCCAAGAGGAAGAAGAAAGTGTTAAAAAGGAACGTCAAGGTCGTGGGAAAT
  AAAAGGCAGTTCTACACAGACTGCAGCAACGGTTGCATCTGCATATCCTAAGAGGAAAAA
  ATGACCTTCAAGAGAATTAGGACTTTTTTCTTAATTTCACTGACTTCAGAGACGATTGCA
  GACTTGCAGTTTAAGTATTGGAATTTCACAAAAGACATAGGACTTAACTGGAAAATGAAA
  AAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_016258
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016258.2, NP_057342.2
• RefSeq Size: 3073 bp
• RefSeq ORF: 1740 bp
• Locus ID: 51441
• UniProt ID: Q9Y5A9
• Cytogenetics: 1p35.3
• Domains: YTH
• Gene Summary: This gene encodes a member of the YTH (YT521-B homology) superfamily containing YTH domain. The YTH domain is typical for the eukaryotes and is particularly abundant in plants. The YTH domain is usually located in the middle of the protein sequence and may function in binding to RNA. In addition to a YTH domain, this protein has a proline rich region which may be involved in signal transduction. An Alu-rich domain has been identified in one of the introns of this gene, which is thought to be associated with human longevity. In addition, reciprocal translocations between this gene and the Runx1 (AML1) gene on chromosome 21 has been observed in patients with acute myeloid leukemia. This gene was initially mapped to chromosome 14, which was later turned out to be a pseudogene. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Oct 2012]
  Transcript Variant: This variant (1) encodes the longer isoform (1).