DDX54 (NM_001111322) Human Untagged Clone

SKU SC318998

DDX54 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 54 (DDX54), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: DDX54
• Synonyms: DP97
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC318998 representing NM_001111322.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGCGGCCGACAAGGGCCCGGCGGCTGGACCTCGGTCGCGAGCTGCCATGGCCCAGTGGAGGAAGAAG; AAAGGGCTCCGGAAGCGCCGAGGCGCGGCCTCCCAGGCCCGCGGCAGCGACTCGGAGGACGGCGAGTTT; GAGATCCAGGCGGAAGATGACGCCCGGGCCCGGAAGCTGGGACCTGGAAGACCCCTGCCCACCTTCCCC; ACCTCGGAATGCACCTCGGATGTGGAGCCGGACACCCGGGAGATGGTGCGTGCCCAGAACAAGAAGAAG; AAGAAGTCTGGAGGCTTCCAGTCCATGGGCCTGAGCTACCCGGTGTTCAAAGGCATCATGAAGAAGGGG; TACAAGGTGCCAACACCCATCCAGAGGAAGACCATCCCGGTGATCTTGGATGGCAAGGACGTGGTGGCC; ATGGCCCGGACGGGCAGTGGCAAGACAGCCTGCTTCCTCCTCCCAATGTTCGAGCGGCTCAAGACCCAC; AGTGCCCAGACCGGGGCCCGCGCCCTCATCCTCTCGCCGACCCGAGAGCTGGCCCTGCAGACCCTGAAG; TTCACTAAGGAGCTAGGCAAGTTCACTGGCCTCAAGACTGCCCTGATCCTGGGTGGAGACAGGATGGAA; GACCAGTTTGCAGCCCTGCACGAAAATCCCGACATAATTATTGCCACGCCCGGACGGTTGGTGCATGTG; GCTGTGGAAATGAGCCTGAAGCTGCAGAGTGTGGAATACGTGGTGTTCGATGAAGCTGACCGGCTTTTT; GAAATGGGTTTCGCAGAGCAGCTGCAGGAGATCATCGCCCGCCTCCCCGGGGGCCACCAGACGGTGCTG; TTCTCCGCCACGCTGCCCAAACTGCTGGTGGAATTTGCCCGGGCTGGCCTCACGGAGCCCGTGCTCATC; CGGCTTGACGTGGATACCAAGCTCAACGAGCAGCTGAAGACCTCCTTCTTCCTCGTGCGGGAGGACACC; AAGGCTGCCGTGCTGCTCCACCTGCTGCACAACGTGGTGCGGCCCCAGGACCAGACCGTGGTGTTTGTG; GCCACGAAGCACCACGCCGAGTACCTCACTGAGCTGCTGACGACCCAGCGGGTGAGCTGCGCCCACATC; TACAGTGCCCTAGACCCGACAGCCCGCAAGATCAATCTCGCCAAATTCACGCTTGGCAAGTGCTCCACT; CTCATTGTGACTGACCTGGCCGCCCGAGGCCTGGACATCCCGCTGCTGGACAATGTCATCAACTACAGC; TTCCCCGCCAAGGGCAAACTCTTCCTGCACCGCGTGGGCCGTGTGGCTCGGGCTGGCCGAAGTGGCACA; GCCTACTCCTTGGTGGCCCCTGATGAAATCCCCTACCTGCTGGATCTGCACCTGTTCCTGGGCCGCTCC; CTCACCCTCGCCCGACCCCTCAAGGAGCCCTCAGGTGTGGCCGGTGTGGATGGCATGCTGGGTCGGGTG; CCACAGAGTGTGGTGGACGAGGAGGACAGTGGTCTGCAGAGCACCCTGGAGGCATCGCTGGAGCTACGG; GGCCTGGCCCGCGTTGCTGATAACGCCCAGCAGCAGTATGTGCGCTCACGCCCGGCGCCCTCGCCTGAG; TCCATCAAGAGGGCCAAGGAGATGGACCTTGTGGGGCTGGGCCTGCACCCCCTCTTCAGCTCGCGTTTT; GAGGAGGAGGAGCTGCAGCGGCTGAGGCTGGTGGACAGCATAAAGAACTACCGCTCCCGGGCGACTATC; TTTGAGATCAACGCCTCCAGCCGAGACCTGTGCAGCCAGGTGATGCGCGCCAAGCGGCAGAAGGACCGC; AAGGCCATCGCCCGCTTCCAGCAGGGACAGCAGGGGCGGCAGGAGCAGCAGGAGGGCCCAGTGGGCCCA; GCCCCGAGCCGCCCAGCACTGCAGGAGAAGCAGCCTGAGAAGGAGGAGGAGGAGGAGGCGGGAGAGAGT; GTGGAGGACATTTTCTCAGAGGTCGTGGGCCGGAAGCGGCAGCGGTCAGGACCCAACAGGGGAGCCAAG; AGGCGGAGGGAGGAGGCCCGGCAGCGGGACCAGGAATTCTACATCCCCTACCGGCCCAAGGACTTTGAC; AGCGAGCGGGGCCTGAGCATCAGCGGGGAAGGGGGAGCCTTTGAGCAGCAGGCAGCTGGCGCTGTCCTG; GACTTGATGGGGGATGAAGCCCAGAACCTGACGAGGGGCCGGCAGCAGCTCAAGTGGGACCGTAAGAAG; AAGCGGTTTGTGGGACAGTCAGGACAGGAAGACAAGAAGAAGATTAAGACAGAGAGCGGCCGCTACATC; AGCAGCTCCTACAAGCGAGACCTCTATCAGAAGTGGAAACAGAAACAGAAAATTGATGATCGTGACTCG; GACGAAGAAGGGGCATCTGACCGGCGAGGCCCAGAGCGAAGAGGTGGGAAGCGAGACCGTGGCCAAGCA; GGTGCATCCCGGCCCCACGCCCCAGGCACCCCTGCAGGCCGAGTCCGCCCGGAACTCAAGACCAAGCAG; CAGATCCTGAAGCAGCGGCGCCGGGCCCAGAAGCTGCACTTCCTGCAGCGTGGTGGCCTCAAGCAGCTC; TCTGCCCGCAACCGCCGCCGCGTCCAGGAGCTGCAGCAGGGCGCCTTCGGCCGGGGTGCCCGCTCCAAG; AAGGGCAAGATGCGGAAGAGGATGTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001111322
• Insert Size: 2649 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001111322.1
• RefSeq Size: 4404 bp
• RefSeq ORF: 2649 bp
• Locus ID: 79039
• UniProt ID: Q8TDD1
• Cytogenetics: 12q24.13
• Protein Families: Druggable Genome, Transcription Factors
• MW: 98.7 kDa
• Summary: This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The nucleolar protein encoded by this gene interacts in a hormone-dependent manner with nuclear receptors, and represses their transcriptional activity. Alternative splice variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).