HIPK2 (NM_022740) Human Untagged Clone

CAT#: SC318547

HIPK2 (untagged)-Human homeodomain interacting protein kinase 2 (HIPK2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HIPK2
• Synonyms: PRO0593
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC318547 representing NM_022740.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCCCCGTGTACGAAGGTATGGCCTCACATGTGCAAGTTTTCTCCCCTCACACCCTTCAATCAAGT
  GCCTTCTGTAGTGTGAAGAAACTGAAAATAGAGCCGAGTTCCAACTGGGACATGACTGGGTACGGCTCC
  CACAGCAAAGTGTATAGCCAGAGCAAGAACATCCCCCTGTCGCAGCCAGCCACCACAACCGTCAGCACC
  TCCTTGCCGGTCCCAAACCCAAGCCTACCTTACGAGCAGACCATCGTCTTCCCAGGAAGCACCGGGCAC
  ATCGTGGTCACCTCAGCAAGCAGCACTTCTGTCACCGGGCAAGTCCTCGGCGGACCACACAACCTAATG
  CGTCGAAGCACTGTGAGCCTCCTTGATACCTACCAAAAATGTGGACTCAAGCGTAAGAGCGAGGAGATC
  GAGAACACAAGCAGCGTGCAGATCATCGAGGAGCATCCACCCATGATTCAGAATAATGCAAGCGGGGCC
  ACTGTCGCCACTGCCACCACGTCTACTGCCACCTCCAAAAACAGCGGCTCCAACAGCGAGGGCGACTAT
  CAGCTGGTGCAGCATGAGGTGCTGTGCTCCATGACCAACACCTACGAGGTCTTAGAGTTCTTGGGCCGA
  GGGACGTTTGGGCAAGTGGTCAAGTGCTGGAAACGGGGCACCAATGAGATCGTAGCCATCAAGATCCTG
  AAGAACCACCCATCCTATGCCCGACAAGGTCAGATTGAAGTGAGCATCCTGGCCCGGTTGAGCACGGAG
  AGTGCCGATGACTATAACTTCGTCCGGGCCTACGAATGCTTCCAGCACAAGAACCACACGTGCTTGGTC
  TTCGAGATGTTGGAGCAGAACCTCTATGACTTTCTGAAGCAAAACAAGTTTAGCCCCTTGCCCCTCAAA
  TACATTCGCCCAGTTCTCCAGCAGGTAGCCACAGCCCTGATGAAACTCAAAAGCCTAGGTCTTATCCAC
  GCTGACCTCAAACCAGAAAACATCATGCTGGTGGATCCATCTAGACAACCATACAGAGTCAAGGTCATC
  GACTTTGGTTCAGCCAGCCACGTCTCCAAGGCTGTGTGCTCCACCTACTTGCAGTCCAGATATTACAGG
  GCCCCTGAGATCATCCTTGGTTTACCATTTTGTGAGGCAATTGACATGTGGTCCCTGGGCTGTGTTATT
  GCAGAATTGTTCCTGGGTTGGCCGTTATATCCAGGAGCTTCGGAGTATGATCAGATTCGGTATATTTCA
  CAAACACAGGGTTTGCCTGCTGAATATTTATTAAGCGCCGGGACAAAGACAACTAGGTTTTTCAACCGT
  GACACGGACTCACCATATCCTTTGTGGAGACTGAAGACACCAGATGACCATGAAGCAGAGACAGGGATT
  AAGTCAAAAGAAGCAAGAAAGTACATTTTCAACTGTTTAGATGATATGGCCCAGGTGAACATGACGACA
  GATTTGGAAGGGAGCGACATGTTGGTAGAAAAGGCTGACCGGCGGGAGTTCATTGACCTGTTGAAGAAG
  ATGCTGACCATTGATGCTGACAAGAGAATCACTCCAATCGAAACCCTGAACCATCCCTTTGTCACCATG
  ACACACTTACTCGATTTTCCCCACAGCACACACGTCAAATCATGTTTCCAGAACATGGAGATCTGCAAG
  CGTCGGGTGAATATGTATGACACGGTGAACCAGAGCAAAACCCCTTTCATCACGCACGTGGCCCCCAGC
  ACGTCCACCAACCTGACCATGACCTTTAACAACCAGCTGACCACTGTCCACAACCAGGCTCCCTCCTCT
  ACCAGTGCCACTATTTCCTTAGCCAATCCCGAAGTCTCCATACTAAACTACCCATCTACACTCTACCAG
  CCCTCAGCGGCATCCATGGCTGCAGTGGCCCAGCGGAGCATGCCCCTGCAGACAGGAACAGCCCAGATT
  TGTGCCCGGCCTGACCCGTTCCAGCAAGCTCTCATCGTGTGTCCCCCCGGCTTCCAAGGCTTGCAGGCC
  TCTCCCTCTAAGCACGCTGGCTACTCGGTGCGAATGGAAAATGCAGTTCCCATCGTCACTCAAGCCCCA
  GGAGCTCAGCCTCTTCAGATCCAACCAGGTCTGCTTGCCCAGCAGGCTTGGCCAAGTGGGACCCAGCAG
  ATCCTGCTTCCCCCAGCATGGCAGCAACTGACTGGAGTGGCCACCCACACATCAGTGCAGCATGCCACC
  GTGATTCCCGAGACCATGGCAGGCACCCAGCAGCTGGCGGACTGGAGAAATACGCATGCTCACGGAAGC
  CATTATAATCCCATCATGCAGCAGCCTGCACTATTGACCGGTCATGTGACCCTTCCAGCAGCACAGCCC
  TTAAATGTGGGTGTGGCCCACGTGATGCGGCAGCAGCCAACCAGCACCACCTCCTCCCGGAAGAGTAAG
  CAGCACCAGTCATCTGTGAGAAATGTCTCCACCTGTGAGGTGTCCTCCTCTCAGGCCATCAGCTCCCCA
  CAGCGATCCAAGCGTGTCAAGGAGAACACACCTCCCCGCTGTGCCATGGTGCACAGTAGCCCGGCCTGC
  AGCACCTCGGTCACCTGTGGGTGGGGCGACGTGGCCTCCAGCACCACCCGGGAACGGCAGCGGCAGACA
  ATTGTCATTCCCGACACTCCCAGCCCCACGGTCAGCGTCATCACCATCAGCAGTGACACGGACGAGGAG
  GAGGAACAGAAACACGCCCCCACCAGCACTGTCTCCAAGCAAAGAAAAAACGTCATCAGCTGTGTCACA
  GTCCACGACTCCCCCTACTCCGACTCCTCCAGCAACACCAGCCCCTACTCCGTGCAGCAGCGTGCTGGG
  CACAACAATGCCAATGCCTTTGACACCAAGGGGAGCCTGGAGAATCACTGCACGGGGAACCCCCGAACC
  ATCATCGTGCCACCCCTGAAAACCCAGGCCAGCGAAGTATTGGTGGAGTGTGATAGCCTGGTGCCAGTC
  AACACCAGTCACCACTCGTCCTCCTACAAGTCCAAGTCCTCCAGCAACGTGACCTCCACCAGCGGTCAC
  TCTTCAGGGAGCTCATCTGGAGCCATCACCTACCGGCAGCAGCGGCCGGGCCCCCACTTCCAGCAGCAG
  CAGCCACTCAATCTCAGCCAGGCTCAGCAGCACATCACCACGGACCGCACTGGGAGCCACCGAAGGCAG
  CAGGCCTACATCACTCCCACCATGGCCCAGGCTCCGTACTCCTTCCCGCACAACAGCCCCAGCCACGGC
  ACTGTGCACCCGCATCTGGCTGCAGCCGCTGCCGCTGCCCACCTCCCCACCCAGCCCCACCTCTACACC
  TACACTGCGCCGGCGGCCCTGGGCTCCACCGGCACCGTGGCCCACCTGGTGGCCTCGCAAGGCTCTGCG
  CGCCACACCGTGCAGCACACTGCCTACCCAGCCAGCATCGTCCACCAGGTCCCCGTGAGCATGGGCCCC
  CGGGTCCTGCCCTCGCCCACCATCCACCCGAGTCAGTATCCAGCCCAATTTGCCCACCAGACCTACATC
  AGCGCCTCGCCAGCCTCCACCGTCTACACTGGATACCCACTGAGCCCCGCCAAGGTCAACCAGTACCCT
  TACATATAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_022740
• Insert Size: 3597 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022740.4
• RefSeq Size: 15245 bp
• RefSeq ORF: 3597 bp
• Locus ID: 28996
• UniProt ID: Q9H2X6
• Cytogenetics: 7q34
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• MW: 131 kDa
• Gene Summary: This gene encodes a conserved serine/threonine kinase that is a member of the homeodomain-interacting protein kinase family. The encoded protein interacts with homeodomain transcription factors and many other transcription factors such as p53, and can function as both a corepressor and a coactivator depending on the transcription factor and its subcellular localization. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
  Transcript Variant: This variant (1) encodes the full-length isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.